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- PDB-7m2h: Structural Snapshots of Intermediates in the Gating of a K+ Channel -
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Open data
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Basic information
Entry | Database: PDB / ID: 7m2h | ||||||
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Title | Structural Snapshots of Intermediates in the Gating of a K+ Channel | ||||||
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![]() | TRANSPORT PROTEIN/IMMUNE SYSTEM / Ion channel / K+ channel / pH gated ion channel / TRANSPORT PROTEIN-IMMUNE SYSTEM complex | ||||||
Function / homology | ![]() delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Reddi, R. / Valiyaveetil, F.I. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structures of Gating Intermediates in a K + channell. Authors: Reddi, R. / Matulef, K. / Riederer, E. / Moenne-Loccoz, P. / Valiyaveetil, F.I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 220.4 KB | Display | ![]() |
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PDB format | ![]() | 171.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 4.8 MB | Display | ![]() |
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Full document | ![]() | 4.8 MB | Display | |
Data in XML | ![]() | 39.9 KB | Display | |
Data in CIF | ![]() | 54.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7m2iC ![]() 7m2jC ![]() 7rp0C ![]() 1k4cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein , 1 types, 2 molecules CF
#3: Protein | Mass: 13329.734 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Antibody , 2 types, 4 molecules ADBE
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Antibody | Mass: 23435.738 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 5 types, 145 molecules ![](data/chem/img/F09.gif)
![](data/chem/img/K.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/K.gif)
![](data/chem/img/DGA.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | #5: Chemical | ChemComp-K / #6: Chemical | #7: Chemical | #8: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.67 Å3/Da / Density % sol: 66.51 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.25 Details: 50 mM MES, pH 6.25, 28% PEG400, 50 mM magnesium acetate PH range: 6.0-6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 23, 2018 |
Radiation | Monochromator: Double crystal cryo-cooled Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.04 Å / Relative weight: 1 |
Reflection | Resolution: 2.64→48.801 Å / Num. obs: 54531 / % possible obs: 99.9 % / Redundancy: 6.9 % / CC1/2: 0.99 / Net I/σ(I): 9.1 |
Reflection shell | Resolution: 2.64→2.73 Å / Mean I/σ(I) obs: 1 / Num. unique obs: 4451 / CC1/2: 0.43 |
-Phasing
Phasing | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1K4C Resolution: 2.642→48.801 Å / SU ML: 0.41 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 31.07 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 106.63 Å2 / Biso mean: 38.3112 Å2 / Biso min: 6.67 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.642→48.801 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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