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Open data
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Basic information
Entry | Database: PDB / ID: 2dwe | ||||||
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Title | Crystal structure of KcsA-FAB-TBA complex in Rb+ | ||||||
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![]() | MEMBRANE PROTEIN / POTASSIUM CHANNEL / TETRABUTYLAMMONIUM / K+ / KcsA | ||||||
Function / homology | ![]() delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Yohannan, S. / Zhou, Y. | ||||||
![]() | ![]() Title: Crystallographic Study of the Tetrabutylammonium Block to the KcsA K(+) Channel Authors: Yohannan, S. / Hu, Y. / Zhou, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 121.9 KB | Display | ![]() |
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PDB format | ![]() | 92 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 715.2 KB | Display | ![]() |
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Full document | ![]() | 735.6 KB | Display | |
Data in XML | ![]() | 25 KB | Display | |
Data in CIF | ![]() | 34 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2dwdC ![]() 2hvjC ![]() 2hvkC ![]() 1k4cS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | biological assembly is a tetramer generated by: chain A, chain B (x,y,z (1_555),2-x,2-y,z (2_775),2-y,x,z (3_755),y,2-x,z (4_575)); chain C (x,y,z (1_555),2-x,2-y,z (2_775),2-y,x,z (3_755),y,2-x,z (4_575)) |
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Components
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 10978.736 Da / Num. of mol.: 1 / Fragment: residues 22-124 / Mutation: L90C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: hybridoma cell line / Source: (natural) ![]() ![]() |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: hybridoma cell line / Source: (natural) ![]() ![]() |
-Non-polymers , 5 types, 103 molecules ![](data/chem/img/RB.gif)
![](data/chem/img/L2C.gif)
![](data/chem/img/F09.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/L2C.gif)
![](data/chem/img/F09.gif)
![](data/chem/img/TBA.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | #5: Chemical | ChemComp-L2C / ( | #6: Chemical | ChemComp-F09 / | #7: Chemical | ChemComp-TBA / | #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.91 Å3/Da / Density % sol: 68.57 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 18-25% PEG 400, 50mM MG(AC)2, 50mM NaAc(pH 5) or Na cacodylate(pH 6) or HEPES(pH 7), pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 1, 2005 |
Radiation | Monochromator: Si-111 double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→26.84 Å / Num. all: 30997 / Num. obs: 30341 / % possible obs: 97.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Rmerge(I) obs: 0.06 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 4.2 % / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1K4C Resolution: 2.5→26.84 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.5→26.84 Å
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Refine LS restraints |
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