+Open data
-Basic information
Entry | Database: PDB / ID: 2hvj | ||||||
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Title | Crystal structure of KcsA-Fab-TBA complex in low K+ | ||||||
Components |
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Keywords | MEMBRANE PROTEIN / potassium channel / tetrabutylammonium / K+ / KcsA | ||||||
Function / homology | Function and homology information monoatomic ion transmembrane transport / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Streptomyces lividans (bacteria) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.75 Å | ||||||
Authors | Zhou, Y. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2007 Title: Crystallographic Study of the Tetrabutylammonium Block to the KcsA K(+) Channel. Authors: Yohannan, S. / Hu, Y. / Zhou, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2hvj.cif.gz | 120.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2hvj.ent.gz | 91 KB | Display | PDB format |
PDBx/mmJSON format | 2hvj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hv/2hvj ftp://data.pdbj.org/pub/pdb/validation_reports/hv/2hvj | HTTPS FTP |
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-Related structure data
Related structure data | 2dwdC 2dweC 2hvkC 1k4cS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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Details | Chain A and B assemble to form the biological unit of Fab. / The biological assembly is a tetramer generated from the monomer in the asymmetric unit by the operations: -X,-Y,Z -Y,X,Z Y,-X,Z 1/2+X,1/2+Y,1/2+Z 1/2-X,1/2-Y,1/2+Z 1/2-Y,1/2+X,1/2+Z 1/2+Y,1/2-X,1/2+Z |
-Components
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 13211.582 Da / Num. of mol.: 1 / Mutation: P2A, L90C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces lividans (bacteria) / Gene: kcsA / Plasmid: pQE60 / Production host: Escherichia coli (E. coli) / Strain (production host): XL1Blue / References: UniProt: P0A334 |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: hybridoma cell line / Source: (natural) Mus musculus (house mouse) |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: hybridoma cell line / Source: (natural) Mus musculus (house mouse) |
-Non-polymers , 5 types, 70 molecules
#4: Chemical | ChemComp-F09 / | ||||||
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#5: Chemical | #6: Chemical | ChemComp-L2C / ( | #7: Chemical | ChemComp-TBA / | #8: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.86 Å3/Da / Density % sol: 68.12 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 6.5 Details: 18-25% PEG 400, 50mM Mg(Ac)2, 50mM NaAc (pH5) or Na Cacodylate (pH6) or Hepes (PH 7), pH 6.5, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Nov 28, 2005 / Details: mirrors |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.75→40 Å / Num. all: 23980 / Num. obs: 24022 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.7 % / Rmerge(I) obs: 0.08 / Χ2: 1.04 / Net I/σ(I): 13.5 |
Reflection shell | Resolution: 2.75→2.85 Å / Redundancy: 6.1 % / Rmerge(I) obs: 0.37 / Num. unique all: 2420 / Χ2: 0.991 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1K4C Resolution: 2.75→40 Å / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 49.09 Å2 | ||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.75→40 Å
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Refine LS restraints |
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