- PDB-7roy: The structure of the Fem1B:FNIP1 complex -
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Basic information
Entry
Database: PDB / ID: 7roy
Title
The structure of the Fem1B:FNIP1 complex
Components
Folliculin-interacting protein 1
Protein fem-1 homolog B
Keywords
SIGNALING PROTEIN / Complex / Redox sensor / Stress response
Function / homology
Function and homology information
Amino acids regulate mTORC1 / regulation of ubiquitin-protein transferase activity / epithelial cell maturation involved in prostate gland development / positive regulation of B cell apoptotic process / regulation of pro-B cell differentiation / branching involved in prostate gland morphogenesis / immature B cell differentiation / Neddylation / ATPase inhibitor activity / regulation of DNA damage checkpoint ...Amino acids regulate mTORC1 / regulation of ubiquitin-protein transferase activity / epithelial cell maturation involved in prostate gland development / positive regulation of B cell apoptotic process / regulation of pro-B cell differentiation / branching involved in prostate gland morphogenesis / immature B cell differentiation / Neddylation / ATPase inhibitor activity / regulation of DNA damage checkpoint / death receptor binding / regulation of extrinsic apoptotic signaling pathway via death domain receptors / negative regulation of TOR signaling / epithelial cell maturation / positive regulation of TOR signaling / TOR signaling / cellular response to starvation / guanyl-nucleotide exchange factor activity / regulation of protein phosphorylation / positive regulation of protein-containing complex assembly / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / positive regulation of peptidyl-serine phosphorylation / protein-folding chaperone binding / protein ubiquitination / positive regulation of protein phosphorylation / lysosomal membrane / apoptotic process / enzyme binding / negative regulation of transcription by RNA polymerase II / nucleoplasm / nucleus / cytoplasm / cytosol Similarity search - Function
Folliculin-interacting protein family / Folliculin-interacting protein, N-terminal domain / Folliculin-interacting protein, middle domain / Folliculin-interacting protein, C-terminal domain / Folliculin-interacting protein N-terminus / Folliculin-interacting protein middle domain / Folliculin-interacting protein C-terminus / Tripartite DENN domain, FNIP1/2-type / Tripartite DENN FNIP1/2-type domain profile. / Domain of unknown function DUF3447 ...Folliculin-interacting protein family / Folliculin-interacting protein, N-terminal domain / Folliculin-interacting protein, middle domain / Folliculin-interacting protein, C-terminal domain / Folliculin-interacting protein N-terminus / Folliculin-interacting protein middle domain / Folliculin-interacting protein C-terminus / Tripartite DENN domain, FNIP1/2-type / Tripartite DENN FNIP1/2-type domain profile. / Domain of unknown function DUF3447 / Ankyrin repeat / Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily Similarity search - Domain/homology
A: Protein fem-1 homolog B B: Protein fem-1 homolog B C: Protein fem-1 homolog B D: Protein fem-1 homolog B G: Folliculin-interacting protein 1 H: Folliculin-interacting protein 1 hetero molecules
Proteinfem-1homologB / FEM1b / FEM1-beta / Fem-1-like death receptor-binding protein alpha / Fem-1-like in apoptotic ...FEM1b / FEM1-beta / Fem-1-like death receptor-binding protein alpha / Fem-1-like in apoptotic pathway protein alpha / F1A-alpha / mt-Fem
Mass: 42362.277 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Fem1b, F1aa, Kiaa0396 / Plasmid: pETDuet1 / Production host: Escherichia coli (E. coli) / Strain (production host): LOBSTR / References: UniProt: Q9Z2G0
#2: Protein/peptide
Folliculin-interactingprotein1
Mass: 3504.970 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Fnip1, Kiaa1961 / Plasmid: pETDuet1 / Production host: Escherichia coli (E. coli) / Strain (production host): LOBSTR / References: UniProt: Q68FD7
Mass: 18.015 Da / Num. of mol.: 17 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 4.46 Å3/Da / Density % sol: 72.42 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop Details: FEM1B-FNIP1 complex (20 mg/mL) was mixed in a 1:1 ratio with the reservoir solution containing 6% isopropanol, 150 mM NaCl, and 100 mM HEPES-NaOH pH 7.5
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
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