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- PDB-7rd5: Crystal structure of Tspan15 large extracellular loop (Tspan15 LE... -

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Basic information

Entry
Database: PDB / ID: 7rd5
TitleCrystal structure of Tspan15 large extracellular loop (Tspan15 LEL) in complex with 1C12 Fab
Components
  • 1C12 Fab Heavy Chain
  • 1C12 Fab Light Chain
  • Tetraspanin-15
KeywordsCELL ADHESION / Tetraspanin / Membrane Protein / Protein Trafficking
Function / homology
Function and homology information


regulation of membrane protein ectodomain proteolysis / tetraspanin-enriched microdomain / negative regulation of Notch signaling pathway / protein maturation / protein localization to plasma membrane / late endosome membrane / cell junction / nuclear body / endoplasmic reticulum lumen / Amyloid fiber formation ...regulation of membrane protein ectodomain proteolysis / tetraspanin-enriched microdomain / negative regulation of Notch signaling pathway / protein maturation / protein localization to plasma membrane / late endosome membrane / cell junction / nuclear body / endoplasmic reticulum lumen / Amyloid fiber formation / intracellular membrane-bounded organelle / enzyme binding / cell surface / plasma membrane / cytosol
Similarity search - Function
Cd81 Antigen, Extracellular Domain; Chain: A / Tetraspanin / Tetraspanin, animals / Tetraspanin, EC2 domain superfamily / Tetraspanin/Peripherin / Tetraspanin family / Immunoglobulins / Immunoglobulin-like / Sandwich / Orthogonal Bundle ...Cd81 Antigen, Extracellular Domain; Chain: A / Tetraspanin / Tetraspanin, animals / Tetraspanin, EC2 domain superfamily / Tetraspanin/Peripherin / Tetraspanin family / Immunoglobulins / Immunoglobulin-like / Sandwich / Orthogonal Bundle / Mainly Beta / Mainly Alpha
Similarity search - Domain/homology
Biological speciesMus musculus (house mouse)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.6 Å
AuthorsLipper, C.H. / Gabriel, K.H. / Seegar, T.C.M. / Durr, K.L. / Tomlinson, M.G. / Blacklow, S.C.
Funding support United States, United Kingdom, 4items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)R35CA220340 United States
Other privateEdward B. Goodnow United States
Biotechnology and Biological Sciences Research Council (BBSRC)BB/P00783X/1 United Kingdom
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)P41GM103403 United States
CitationJournal: Structure / Year: 2022
Title: Crystal structure of the Tspan15 LEL domain reveals a conserved ADAM10 binding site.
Authors: Lipper, C.H. / Gabriel, K.H. / Seegar, T.C.M. / Durr, K.L. / Tomlinson, M.G. / Blacklow, S.C.
History
DepositionJul 9, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 3, 2021Provider: repository / Type: Initial release
Revision 1.1Nov 17, 2021Group: Database references / Category: citation
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Feb 16, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year
Revision 1.3Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_asym_id / _struct_ncs_dom_lim.beg_auth_comp_id ..._struct_ncs_dom_lim.beg_auth_asym_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_auth_seq_id / _struct_ncs_dom_lim.end_auth_asym_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_auth_seq_id
Revision 1.4Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 1C12 Fab Light Chain
B: 1C12 Fab Heavy Chain
F: Tetraspanin-15
C: 1C12 Fab Light Chain
D: 1C12 Fab Heavy Chain
E: Tetraspanin-15


Theoretical massNumber of molelcules
Total (without water)126,4246
Polymers126,4246
Non-polymers00
Water00
1
A: 1C12 Fab Light Chain
B: 1C12 Fab Heavy Chain
F: Tetraspanin-15


Theoretical massNumber of molelcules
Total (without water)63,2123
Polymers63,2123
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: 1C12 Fab Light Chain
D: 1C12 Fab Heavy Chain
E: Tetraspanin-15


Theoretical massNumber of molelcules
Total (without water)63,2123
Polymers63,2123
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)171.300, 124.640, 116.120
Angle α, β, γ (deg.)90.000, 98.171, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain "A" and (resid 1 through 80 or (resid 81...
21(chain "C" and (resid 1 through 148 or (resid 149...
12(chain "B" and (resid 1 through 2 or (resid 3...
22(chain "D" and ((resid 1 and (name N or name...
13(chain "F" and (resid 115 through 116 or (resid 117...
23(chain "E" and (resid 115 through 121 or (resid 122...

NCS domain segments:

Component-ID: 1 / End auth comp-ID: GLN / End label comp-ID: GLN

Dom-IDEns-IDBeg auth comp-IDBeg label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ASPASPAA1 - 2201 - 220
21ASPASPCD1 - 2201 - 220
12GLNGLNBB1 - 2261 - 226
22GLNGLNDE1 - 2261 - 226
13THRTHRFC115 - 2361 - 122
23THRTHREF115 - 2361 - 122

NCS ensembles :
ID
1
2
3

NCS oper:
IDCodeMatrixVector
1given(0.999992949462, -0.00358197435106, 0.00112715846479), (0.00358671313799, 0.999984618494, -0.00423063392275), (-0.0011119871052, 0.00423464688858, 0.999990415579)-8.20165972175, 2.72237286895, 57.475859743
2given(0.999983510867, -0.00287683726556, 0.00497009076507), (0.00287672165545, 0.999995861765, 3.04098528697E-5), (-0.00497015768187, -1.61117837041E-5, 0.99998764856)-7.93327737524, 3.04921960376, 57.5023994249
3given(0.999774651704, -0.020609179591, 0.00508994369397), (0.0207577818764, 0.999300433837, -0.0311087997984), (-0.00444525609968, 0.0312074454243, 0.999503044042)-8.3234840046, 1.03211969185, 57.9809027046

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Components

#1: Antibody 1C12 Fab Light Chain


Mass: 24295.867 Da / Num. of mol.: 2 / Mutation: N5Q, 215-220 is an expression tag
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Cell line (production host): Expi293F / Production host: Homo sapiens (human)
#2: Antibody 1C12 Fab Heavy Chain


Mass: 24570.713 Da / Num. of mol.: 2 / Mutation: 221-226 is an expression tag
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Cell line (production host): Expi293F / Production host: Homo sapiens (human)
#3: Protein Tetraspanin-15 / Tspan-15 / Tetraspan NET-7 / Transmembrane 4 superfamily member 15


Mass: 14345.235 Da / Num. of mol.: 2 / Mutation: N118Q, N189D, 231-236 expression tag
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TSPAN15, NET7, TM4SF15, UNQ677/PRO1311 / Cell line (production host): Expi293F / Production host: Homo sapiens (human) / References: UniProt: O95858
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.85 Å3/Da / Density % sol: 74.65 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.6 / Details: 0.1 M sodium acetate pH 4.6, 3.4 M sodium nitrate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.979 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 22, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 3.6→47.5 Å / Num. obs: 27150 / % possible obs: 95.77 % / Redundancy: 2.5 % / Biso Wilson estimate: 121.58 Å2 / CC1/2: 0.995 / CC star: 0.999 / Rmerge(I) obs: 0.1577 / Rpim(I) all: 0.1196 / Rrim(I) all: 0.1991 / Net I/σ(I): 4.63
Reflection shellResolution: 3.6→3.729 Å / Redundancy: 2.5 % / Rmerge(I) obs: 1.08 / Mean I/σ(I) obs: 0.86 / Num. unique obs: 6908 / CC1/2: 0.52 / Rpim(I) all: 0.8215 / Rrim(I) all: 1.364 / % possible all: 97.17

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Processing

Software
NameVersionClassification
PHENIX1.19.1_4122+SVNrefinement
XDSdata reduction
XSCALEdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6bdz

6bdz


Resolution: 3.6→47.5 Å / SU ML: 0.6118 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 38.7174
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.3093 1983 7.34 %
Rwork0.2707 25034 -
obs0.2735 27017 95.63 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 126.93 Å2
Refinement stepCycle: LAST / Resolution: 3.6→47.5 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8279 0 0 0 8279
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00348490
X-RAY DIFFRACTIONf_angle_d0.736411668
X-RAY DIFFRACTIONf_chiral_restr0.04561361
X-RAY DIFFRACTIONf_plane_restr0.0051502
X-RAY DIFFRACTIONf_dihedral_angle_d6.22651208
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
12AX-RAY DIFFRACTIONTorsion NCS0.528930218737
22BX-RAY DIFFRACTIONTorsion NCS0.426172800629
32CX-RAY DIFFRACTIONTorsion NCS0.581515166962
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.6-3.690.41541390.39211761X-RAY DIFFRACTION94.34
3.69-3.790.40381460.37161831X-RAY DIFFRACTION96.72
3.79-3.90.39211390.3571771X-RAY DIFFRACTION95.6
3.9-4.020.42421360.34961707X-RAY DIFFRACTION93.41
4.02-4.170.34661410.32851774X-RAY DIFFRACTION95.27
4.17-4.330.3451440.3041802X-RAY DIFFRACTION97.4
4.33-4.530.32531420.28491806X-RAY DIFFRACTION97.35
4.53-4.770.33911460.25871836X-RAY DIFFRACTION97.2
4.77-5.070.30731430.24931812X-RAY DIFFRACTION96.88
5.07-5.460.2631410.24961777X-RAY DIFFRACTION96.48
5.46-6.010.31121390.26411748X-RAY DIFFRACTION93.65
6.01-6.870.26041460.26421843X-RAY DIFFRACTION97.45
6.87-8.650.27791430.23251811X-RAY DIFFRACTION96.35
8.65-44.710.25341380.21521755X-RAY DIFFRACTION90.92
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.57224211202-1.88074300307-0.2430050939883.615559450960.4345584452212.954141044940.3694748828780.717754953070.810286045518-0.411669685126-0.736410839248-0.739624533682-0.0907491878270.3817699234020.2090745100790.9532274689140.02586583692240.2294651789491.53172155320.155299791321.539919302542.8608832089-26.7891025576-59.8301693178
23.83305737593-0.913999951825-0.1773199047213.78319000649-1.302361797381.62256035710.3167607102530.727074562022-0.03015439132810.0477799319647-0.1235737674790.5880470445750.3949564664020.109142219376-0.2456592708421.124538319950.2556904358140.160829619311.40802756541-0.1374833949571.3524133759467.4474938845-55.9200949468-57.09433871
30.863148946308-0.280563275642-0.5813320819184.37886750031-0.9755458647280.6960336086330.596783829499-1.0812539941-0.5867680669980.461780443853-0.3345909494750.717842770755-0.0682447708411-0.6247358020390.05189573937411.316278350140.07480700953920.0638937145371.66595890968-0.07475560098161.590791806537.1695664256-45.2054580094-40.5995434707
42.03487818442-0.448806277501-0.1998467229785.34571544442-4.045612413453.13440737661-0.335600671623-0.6841574593250.02196156849960.45566136299-0.04787462328650.7609814748630.4055524189490.359417164078-0.234597689591.036177712650.1321247444750.0897703530221.623763894850.131251699391.5013633167529.3528088927-36.5747171106-43.980100894
53.9019803542-1.51986386091-0.4751681561954.776854695980.7995245956622.89888764451-0.436636188482-0.4697123178430.772981693021-0.3304841013590.7454559418880.01574255057530.1428117115280.613052543705-0.1448793429170.9779094011-0.01465288310390.06138469878011.29311491506-0.348921410991.8228132123736.1625909621-36.1398097242-47.5066951794
63.7401794028-1.755094449930.04106463397251.79386809151-0.04609141817653.645803990610.73367514392-0.9317416200761.05943957365-0.258429378912-0.0627775331535-0.486094757559-0.2366004378511.15046696273-0.3273051454621.143766541810.2393032746670.1102918661750.876952945841-0.1916526950531.2054128993559.6435359811-54.5204700751-45.5801771397
72.735349434340.2130401203720.1232991015743.85292362677-1.429447509941.51261431525-0.721529142535-1.025052904780.5059422986810.5462643510230.3456842874280.248448856319-0.0939217333025-0.27393915168-0.03840980564361.386929637370.2290416360580.06759773223611.5654582598-0.1947871301941.6476435534860.2383929591-50.1974356828-43.2718003965
87.887119642134.23184045948-1.184997500353.40100339925-1.058857717110.4129106532190.746569634492-1.91756517907-0.867406084332-0.806289183464-0.652835749276-1.92315986674-0.0461210935395-1.895262194210.02933651433780.6101915894910.176115931319-0.1204180032071.97311519704-0.03096335506561.4229574647276.8921092389-62.8796989276-39.4437333925
95.890581569123.353488463386.89859479233.865378399464.767426403679.250862939170.9279801963990.732103475254-0.703413980668-0.897871544617-0.0883186603603-0.2947663244630.858138174221-0.0552120865522-0.8345926155850.8338281590920.165515085090.2326986116580.798350547058-0.197263072860.9910480252968.99248633585-19.6734028679-32.7474801276
101.80483968512-2.915657699420.6911035011785.845673099320.05959440144241.673314092770.4102336686870.05006904558270.663534966847-1.173308924120.000687118374803-0.571445911628-0.4875620827070.51051760825-0.5593999249061.161084735460.146627194340.2838869854151.32317722216-0.4922834665761.101182926448.54868343765-13.2091214012-45.1160256986
113.493002438312.41582146179-0.4921419073956.37964075896-0.7512805870254.90452309183-0.02120143413320.776954971218-0.181219568016-0.1040610859181.87816667347-0.241599549372-0.03506111835590.226610770172-0.8936657169680.96298628254-0.03701483504930.4934319044461.32494075372-0.3656451941981.8654738075214.4793023438-22.5418695857-50.5635022997
127.04557425846-2.655227271363.490413894664.20133814761-5.52580459667.2687867635-0.140312465526-2.806078361661.760209601411.14733411945-1.44616798899-1.75457507918-1.588288655382.227125970212.346723107521.75481420954-0.214251675848-0.1860506819581.55813655967-0.2876560105861.0303643479219.2656692398-4.83916044134-54.4440790753
133.299081535310.919522874519-2.829987607175.29971028751-1.97547591562.71691119393-0.958112099855-0.0399403456085-1.31375231574-0.7685552401990.243632451373-1.887411369680.73093475736-0.3100201661020.7988824603810.724194603062-0.1245643738590.1424659956981.38810796077-0.2703356961411.6934632781523.7224198705-18.7545767152-50.7862112543
144.604825639051.39244367652-5.152607396465.09083731789-1.597885739245.421183375430.9393139905640.3579146498190.2858490239320.5460933015790.144809077735-1.96659923912-0.1566551938071.68961617938-0.2318589500850.875673540393-0.165685074185-0.3566546384391.5297683116-0.1545610145691.0444245377715.7213930314-6.78154924417-34.6431338023
154.22498869684-1.02827526968-0.7368241776313.304109901242.47051257842.04153426964-0.1421522331390.7890728953980.2809726069980.0160092576849-0.147879169236-0.385517773687-1.056930719240.02856509730320.06727408065470.9021403910610.0663268809162-0.05626262004681.180570953230.08520207908050.91843288486433.9691041623-29.2875374406-9.50966727627
167.309958155371.256548677812.300832832355.860767367551.426934239324.428983263180.246594639102-0.5520115265111.15212017554-0.67484902108-0.310575889611-0.7657512170210.100249311746-0.2699234095950.08232183925020.798964236226-0.06437171667810.02855474307720.8252872427670.2322526344830.87833269069436.0655143556-22.0785245557-1.6002716522
170.6273078712230.602503320188-0.7658937563913.680437497810.4433161079692.28513487016-1.50844328651.80473051428-0.109803958057-0.3041332329461.07452066529-1.006113199450.480471641314-0.3102485060930.3222662244740.760331539704-0.09508819945260.198956243550.905163024917-0.04749973104510.87143464489239.1645337781-30.9392104807-3.97335915083
183.854577202610.9761459262980.004442439356237.12723625553-2.385934498658.183812337240.285341069479-0.2246438939390.56363590830.2150169512350.854015880131.2714618846-0.234005046788-0.252758772065-0.8640652566740.7178514536760.027655768997-0.0322112841780.9410207794970.1016633582980.8282648270356.5049022949-53.31062515381.16193525805
197.140539701090.4171308378682.068980840865.951756717042.224367622564.182469126370.529508579597-1.139031172950.1834075329720.0407474506907-0.08585674106320.600240246830.607791028244-1.581418232420.4667879388090.9994217954490.4262210088340.07432858480541.17352454266-0.02118750659140.96069623792763.8865031095-56.1820083897-4.78774950176
202.68426514734-0.324748380353.040200644181.43955602742-1.803156039945.213687930950.523455343762-0.70359511463-0.1418186355850.175219935688-0.86393538346-0.1223551869521.22069039218-0.348184774795-1.035622188071.11811316240.0676903624365-0.1391107325851.167312255720.5906106926710.39726531700972.4444292929-60.94466128265.84994989656
215.459501751011.416633850110.02996279615626.254695634862.133115603281.524621572040.177684485597-1.55984633497-2.23523852023-0.979637938711-0.609832600928-0.23065803795-0.2375538895960.3125419951310.3011769445150.926479782404-0.0741585876381-0.1260263502481.018238381770.5739583643821.016691484429.8641998056-41.068304787617.1787293211
227.39547449575-1.01628723203-0.06337236930045.15599650031-1.934786058335.076357829-0.441077997296-1.08620453422-0.523850181334-0.315079818950.3133440645070.303563110572-1.03593870419-0.082965262390.437930808540.788854470479-0.0732673676964-0.2025017063770.6990269279020.1558765241030.55280033549521.7075339951-32.93073945211.6934523083
239.346481583540.680876437802-0.8932964178495.03574824421-1.043990009285.225003194040.272287057138-0.850746834735-0.4455046385730.249284857283-0.522128168403-0.954713624641-1.039084874-0.7258094702940.5645601429380.7105220088540.212676913515-0.1953312086270.8691123889440.1728627168970.72557897874225.2339318163-33.979867414713.1223998062
243.46820671308-3.22276271807-0.3851463446753.797049881080.208956938650.2678966281590.6120116513980.439749124472-0.574763638745-1.48372921188-0.2963749909470.550461168791-1.271806820981.86197937162-0.7159311016671.56079972351-0.167678992608-0.2542824700251.188564320530.2194642978891.3623523659134.2648982602-52.93505151713.4810102542
254.85883360607-3.122678280730.5914300127743.304569709310.9344286395342.360961505581.26928839815-0.5355710905942.328623766890.673977467344-0.282024720539-0.118057948852-0.5683265167930.128927152063-0.7364424268271.589307815210.3436846592490.222285149991.15574043969-0.07712945546490.87611341504465.3266974742-50.092656006910.0713493671
264.996576609541.027285587331.205454549744.932090217920.09086576228111.70748662518-0.374920095963-1.406120330480.3669369421890.496421764620.3000672152980.192172788253-0.967509611485-0.692914426440.2465638612161.293585570070.1568325963490.2103851344080.969222533217-0.1539043961510.9370152845153.1081666329-46.451214491512.8073966442
272.341745503330.9080999475212.664794825311.393046742990.9698791794223.07841749377-1.32685880472-0.6531746016130.3909897815180.474320827204-0.4739185262570.0385020840842-0.28789527745-0.832452854034-0.6458720143430.7187653035320.005654289811830.221310651181.199450568710.4728511769460.85819782099859.2684997768-55.502100775219.4735017621
281.85691431231-0.700931097908-1.003383014462.8147852306-0.17738427053.19390190787-0.765185661495-0.2349219613820.259606377212-0.2843778013070.00548403280849-0.05835815268560.4312663842770.274637108424-0.3397607915050.8725853993820.328209937507-1.072686720491.22761014466-0.365279939152-0.954015302334-0.806592793238-13.630690629317.281340362
297.260103718141.46641798468-1.915960713453.14126636112-0.6185645804753.59897044531-0.147096839473-0.480755950680.505617498219-0.166793591773-0.0936894048392-0.2534199026960.220411583223-0.0197593153371-0.1016926872750.7234816986910.104269850668-0.199074595550.758900881222-0.03876531616780.7221054266398.89855276252-11.621600798712.1923406407
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 102 )AA1 - 1021 - 102
22chain 'A' and (resid 103 through 220 )AA103 - 220103 - 220
33chain 'B' and (resid 1 through 17 )BB1 - 171 - 17
44chain 'B' and (resid 18 through 83 )BB18 - 8318 - 83
55chain 'B' and (resid 84 through 111 )BB84 - 11184 - 111
66chain 'B' and (resid 112 through 140 )BB112 - 140112 - 140
77chain 'B' and (resid 141 through 214 )BB141 - 214141 - 214
88chain 'B' and (resid 215 through 226 )BB215 - 226215 - 226
99chain 'F' and (resid 115 through 133 )FC115 - 1331 - 19
1010chain 'F' and (resid 134 through 159 )FC134 - 15920 - 45
1111chain 'F' and (resid 160 through 185 )FC160 - 18546 - 71
1212chain 'F' and (resid 186 through 195 )FC186 - 19572 - 81
1313chain 'F' and (resid 196 through 210 )FC196 - 21082 - 96
1414chain 'F' and (resid 211 through 236 )FC211 - 23697 - 122
1515chain 'C' and (resid 1 through 14 )CD1 - 141 - 14
1616chain 'C' and (resid 15 through 90 )CD15 - 9015 - 90
1717chain 'C' and (resid 91 through 113 )CD91 - 11391 - 113
1818chain 'C' and (resid 114 through 186 )CD114 - 186114 - 186
1919chain 'C' and (resid 187 through 204 )CD187 - 204187 - 204
2020chain 'C' and (resid 205 through 220 )CD205 - 220205 - 220
2121chain 'D' and (resid 1 through 16 )DE1 - 161 - 16
2222chain 'D' and (resid 17 through 67 )DE17 - 6717 - 67
2323chain 'D' and (resid 68 through 111 )DE68 - 11168 - 111
2424chain 'D' and (resid 112 through 124 )DE112 - 124112 - 124
2525chain 'D' and (resid 125 through 140 )DE125 - 140125 - 140
2626chain 'D' and (resid 141 through 204 )DE141 - 204141 - 204
2727chain 'D' and (resid 205 through 226 )DE205 - 226205 - 226
2828chain 'E' and (resid 115 through 152 )EF115 - 1521 - 38
2929chain 'E' and (resid 153 through 236 )EF153 - 23639 - 122

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