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- PDB-7qjm: Crystal structure of an alpha/beta-hydrolase enzyme from Chlorofl... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7qjm | ||||||
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Title | Crystal structure of an alpha/beta-hydrolase enzyme from Chloroflexus sp. MS-G (202) | ||||||
![]() | alpha/beta-hydrolase (202) | ||||||
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Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zahn, M. / Graham, R. / Beckham, G.T. / McGeehan, J.E. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Sourcing thermotolerant poly(ethylene terephthalate) hydrolase scaffolds from natural diversity Authors: Erickson, E. / Gado, J.E. / Avilan, L. / Bratti, F. / Brizendine, R.K. / Cox, P.A. / Gill, R. / Graham, R. / Kim, D.J. / Konig, G. / Michener, W.E. / Poudel, S. / Ramirez, K.J. / ...Authors: Erickson, E. / Gado, J.E. / Avilan, L. / Bratti, F. / Brizendine, R.K. / Cox, P.A. / Gill, R. / Graham, R. / Kim, D.J. / Konig, G. / Michener, W.E. / Poudel, S. / Ramirez, K.J. / Shakespeare, T.J. / Zahn, M. / Boyd, E.S. / Payne, C.M. / DuBois, J.L. / Pickford, A.R. / Beckham, G.T. / McGeehan, J.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 245.9 KB | Display | ![]() |
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PDB format | ![]() | 201 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7qjnC ![]() 7qjoC ![]() 7qjpC ![]() 7qjqC ![]() 7qjrC ![]() 7qjsC ![]() 7qjtC ![]() 7d78S S: Starting model for refinement C: citing same article ( |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 42323.336 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.84 Å3/Da / Density % sol: 56.74 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 15 % PEG 3350, 0.1 M succinic acid |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jan 26, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.19→50.33 Å / Num. obs: 49979 / % possible obs: 100 % / Redundancy: 13.1 % / CC1/2: 0.999 / Rmerge(I) obs: 0.089 / Rpim(I) all: 0.026 / Net I/σ(I): 14.6 |
Reflection shell | Resolution: 2.19→2.23 Å / Redundancy: 13.5 % / Rmerge(I) obs: 3.181 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 2460 / CC1/2: 0.292 / Rpim(I) all: 0.893 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 7d78 Resolution: 2.19→50.33 Å / Cor.coef. Fo:Fc: 0.957 / Cor.coef. Fo:Fc free: 0.935 / SU R Cruickshank DPI: 0.185 / Cross valid method: THROUGHOUT / SU R Blow DPI: 0.182 / SU Rfree Blow DPI: 0.162 / SU Rfree Cruickshank DPI: 0.165
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Displacement parameters | Biso mean: 79.76 Å2
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Refine analyze | Luzzati coordinate error obs: 0.32 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.19→50.33 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.19→2.21 Å
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Refinement TLS params. | Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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