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- PDB-7pl0: Crystal structure of a DyP-type peroxidase 5G5 variant from Bacil... -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 7pl0
TitleCrystal structure of a DyP-type peroxidase 5G5 variant from Bacillus subtilis
ComponentsDeferrochelatase/peroxidase
KeywordsOXIDOREDUCTASE / Dye-decolorizing Peroxidase / Bacillus subtilis / Directed evolution / high-throughput screening / phenolic compounds
Function / homology
Function and homology information


iron import into cell / Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases / peroxidase activity / heme binding / metal ion binding
Similarity search - Function
TAT (twin-arginine translocation) pathway signal sequence / Deferrochelatase / Dyp-type peroxidase, N-terminal / DyP-type peroxidase family. / Dyp-type peroxidase / Twin-arginine translocation pathway, signal sequence, bacterial/archaeal / Dimeric alpha-beta barrel / Twin arginine translocation (Tat) signal profile. / Twin-arginine translocation pathway, signal sequence
Similarity search - Domain/homology
PROTOPORPHYRIN IX CONTAINING FE / Deferrochelatase
Similarity search - Component
Biological speciesBacillus subtilis (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.1 Å
AuthorsBorges, P.T. / Rodrigues, C. / Silva, D. / Taborda, A. / Brissos, V. / Frazao, C. / Martins, L.O.
Funding support United Kingdom, 3items
OrganizationGrant numberCountry
H2020 Marie Curie Actions of the European CommissionB-LigZymes H2020-MSCA-RISE 2018 United Kingdom
Foundation for Science and Technology (FCT)PTDC/BII-BBF/29564/2017 United Kingdom
Foundation for Science and Technology (FCT)PTDC/BBBEBB/0122/2014 United Kingdom
CitationJournal: Int J Mol Sci / Year: 2021
Title: Loops around the Heme Pocket Have a Critical Role in the Function and Stability of Bs DyP from Bacillus subtilis .
Authors: Rodrigues, C.F. / Borges, P.T. / Scocozza, M.F. / Silva, D. / Taborda, A. / Brissos, V. / Frazao, C. / Martins, L.O.
History
DepositionAug 27, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Oct 27, 2021Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Deferrochelatase/peroxidase
B: Deferrochelatase/peroxidase
C: Deferrochelatase/peroxidase
D: Deferrochelatase/peroxidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)185,6258
Polymers183,1594
Non-polymers2,4664
Water15,006833
1
A: Deferrochelatase/peroxidase
B: Deferrochelatase/peroxidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)92,8124
Polymers91,5792
Non-polymers1,2332
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area7070 Å2
ΔGint-61 kcal/mol
Surface area27840 Å2
MethodPISA
2
C: Deferrochelatase/peroxidase
D: Deferrochelatase/peroxidase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)92,8124
Polymers91,5792
Non-polymers1,2332
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6990 Å2
ΔGint-61 kcal/mol
Surface area28300 Å2
MethodPISA
Unit cell
Length a, b, c (Å)63.852, 114.760, 116.819
Angle α, β, γ (deg.)90.000, 104.000, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 56 through 111 or resid 117 through 414))
21chain B
31(chain C and (resid 56 through 111 or resid 117 through 414))
41(chain D and (resid 56 through 308 or resid 322 through 414))
12chain Y
22chain Z

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111GLUGLULYSLYS(chain A and (resid 56 through 111 or resid 117 through 414))AA56 - 11156 - 111
121ARGARGLEULEU(chain A and (resid 56 through 111 or resid 117 through 414))AA117 - 414117 - 414
211GLUGLULEULEUchain BBB56 - 41456 - 414
311GLUGLULYSLYS(chain C and (resid 56 through 111 or resid 117 through 414))CC56 - 11156 - 111
321ARGARGLEULEU(chain C and (resid 56 through 111 or resid 117 through 414))CC117 - 414117 - 414
411GLUGLUGLYGLY(chain D and (resid 56 through 308 or resid 322 through 414))DD56 - 30856 - 308
421PROPROLEULEU(chain D and (resid 56 through 308 or resid 322 through 414))DD322 - 414322 - 414
112HEMHEMHEMHEMchain YDH501
212HEMHEMHEMHEMchain ZCG501

NCS ensembles :
ID
1
2

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Components

#1: Protein
Deferrochelatase/peroxidase


Mass: 45789.742 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: B4417_2151, CFD21_01545 / Production host: Escherichia coli (E. coli)
References: UniProt: A0A162R372, Oxidoreductases; Acting on a peroxide as acceptor; Peroxidases
#2: Chemical
ChemComp-HEM / PROTOPORPHYRIN IX CONTAINING FE / HEME


Mass: 616.487 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C34H32FeN4O4 / Feature type: SUBJECT OF INVESTIGATION
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 833 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.3 Å3/Da / Density % sol: 45.8 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop
Details: 0.1 M Tris-HCl pH 8.5, 25% (w/v) PEG 4K, 0.2 M magnesium chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: MASSIF-3 / Wavelength: 0.968 Å
DetectorType: DECTRIS EIGER X 4M / Detector: PIXEL / Date: Jun 18, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.968 Å / Relative weight: 1
ReflectionResolution: 2.1→61.96 Å / Num. obs: 92499 / % possible obs: 97.13 % / Redundancy: 3.1 % / CC1/2: 0.996 / Rpim(I) all: 0.058 / Rrim(I) all: 0.122 / Rsym value: 0.088 / Net I/σ(I): 9.02
Reflection shellResolution: 2.1→2.2 Å / Num. unique obs: 15038 / CC1/2: 0.478 / Rpim(I) all: 0.352 / Rrim(I) all: 0.1112 / Rsym value: 0.532

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Processing

Software
NameVersionClassification
XSCALEdata scaling
PHENIX1.17.1_3660refinement
PDB_EXTRACT3.27data extraction
XDSdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7PKX

7pkx


Resolution: 2.1→61.96 Å / SU ML: 0.26 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 23.47 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2152 2004 2.17 %
Rwork0.1907 90478 -
obs0.1912 92482 97.13 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 128.73 Å2 / Biso mean: 42.1098 Å2 / Biso min: 13.52 Å2
Refinement stepCycle: final / Resolution: 2.1→61.96 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10725 0 172 833 11730
Biso mean--30.52 43.22 -
Num. residues----1380
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A6569X-RAY DIFFRACTION10.146TORSIONAL
12B6569X-RAY DIFFRACTION10.146TORSIONAL
13C6569X-RAY DIFFRACTION10.146TORSIONAL
14D6569X-RAY DIFFRACTION10.146TORSIONAL
21D16X-RAY DIFFRACTION10.146TORSIONAL
22C16X-RAY DIFFRACTION10.146TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.1-2.150.33061400.3226466660697
2.15-2.210.32691420.28976552669499
2.21-2.280.3121460.27896523666999
2.28-2.350.2831410.26356574671599
2.35-2.430.27811440.25956545668999
2.43-2.530.28621370.23916542667999
2.53-2.650.24121560.22946571672799
2.65-2.790.24051460.22126522666899
2.79-2.960.22941430.21256519666298
2.96-3.190.22321500.19496484663497
3.19-3.510.19581460.18056370651696
3.51-4.020.2081360.15255950608689
4.02-5.060.15631390.13486452659196
5.06-61.960.17061380.16066408654694
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
17.952.43563.95819.5446-2.31153.38280.36150.9071-0.9113-1.2699-0.1766-2.07330.52930.7317-0.30320.32870.11360.11870.47210.01360.529441.764-20.514413.9439
21.27860.6224-0.26141.9580.20241.50970.0469-0.1664-0.14220.125-0.1434-0.2537-0.06080.12060.09220.2159-0.01210.00840.22710.06250.242129.5346-13.961619.538
32.6913-1.1072-1.62575.70980.83978.54680.1369-0.1983-0.12880.63150.0724-0.25540.2087-0.1609-0.12030.3111-0.0409-0.05910.29280.10130.343328.8578-21.935930.8287
41.47641.0098-1.39122.6423-2.96634.0255-0.1299-1.0162-0.8507-0.2920.2036-0.66521.06920.0787-0.20710.5342-0.0332-0.06560.85110.12590.772534.9815-38.523122.8841
53.06172.5604-4.44774.5443-6.02898.6450.0623-0.1809-0.3874-0.1699-0.3873-0.3114-0.01820.4180.33180.40790.01480.01160.27510.02960.323427.9323-37.23519.8416
63.8944-1.7705-3.42321.82361.92165.02340.166-0.126-0.21360.0495-0.32010.1735-0.393-0.08420.16730.2672-0.02890.02980.2730.02320.247622.4065-11.235129.6815
75.3224-0.9357-0.8854.9066-5.12326.20790.5338-0.13830.05990.79960.11310.7079-1.5378-0.8998-0.45620.5976-0.0520.03010.36140.03030.28824.6697-0.486435.1418
87.58261.05684.0775.43373.47755.3523-0.085-0.16610.0753-0.1984-0.11780.6172-0.4864-0.55420.20020.29540.03860.09390.38580.02670.280311.537-1.339918.6894
90.08040.31160.32782.9631-1.41331.73260.1185-0.0494-0.031-0.0738-0.13-0.1763-0.07330.16260.02210.2239-0.00080.04850.27970.010.274324.5291-16.251912.9585
102.64380.0842-0.00261.6227-1.35373.36170.1854-0.19180.040.1094-0.3075-0.3019-0.19620.39130.12250.2998-0.04740.0290.22080.02030.273334.0445-1.067120.8841
113.64240.51030.03583.9831-1.5028.1290.16570.28680.0577-0.5529-0.0570.1999-0.4096-0.2545-0.1550.32160.11190.01530.30090.00620.247715.5986-0.6101-3.5037
121.43250.7640.32212.94380.39122.1657-0.05790.17550.233-0.40130.0125-0.1026-0.4618-0.04370.0290.35550.06180.05990.27220.04280.284725.56124.30314.1494
138.1148-2.5348-0.31021.12471.5347.8349-0.8018-1.21820.5561.33840.30590.5993-1.0367-0.23810.58290.470.10260.01110.468-0.00860.50457.2823-13.20161.855
141.66250.87040.90442.0606-0.45712.02980.0693-0.10030.040.1969-0.1533-0.0595-0.2329-0.01360.10770.3340.03180.05810.25020.00770.233923.35091.454216.2327
152.6819-0.39861.58133.1094-1.93033.97180.1639-0.0650.01410.208-0.16-0.0632-0.22350.10380.0080.2060.00980.06080.2054-0.02510.193624.1241-5.367720.5314
163.7496-2.7757-0.68049.3521-1.42922.0330.09650.1581-0.7409-0.6334-0.0151-0.6299-0.37610.83440.00050.340.03960.14140.38210.0580.28131.2478-23.956-14.3275
171.47430.07230.37472.0261-0.3533.06180.05930.15130.0061-0.2715-0.10230.1908-0.0831-0.1860.05760.32540.0433-0.00220.2533-0.01580.25114.4665-24.5938-13.815
189.8866-3.21665.46767.4484-0.34647.5268-0.23540.7670.2744-0.4283-0.1674-0.3943-0.40820.44330.43410.43680.02720.07590.30180.04490.274821.4108-17.3198-18.569
191.15210.41440.1380.20680.12490.17330.18460.61280.6128-0.4166-0.3461-0.4065-0.1282-0.05290.18560.8260.0329-0.01870.43560.08420.400521.0489-0.6377-15.5344
202.14650.70820.62012.98180.9022.76320.13890.0824-0.117-0.1447-0.07830.3219-0.0633-0.4056-0.02140.29950.07820.00370.2770.01590.189111.4925-23.3504-13.3826
211.28691.5741-0.43584.02910.15465.59780.1497-0.02170.03830.0050.03650.4151-0.0652-0.4199-0.11940.25020.0498-0.0650.3776-0.00530.41582.8888-34.3149-6.6847
221.91040.01851.44414.74021.24813.93560.0477-0.05820.1098-0.1807-0.14040.1598-0.1834-0.26330.10590.26180.03740.06720.25780.01230.227817.062-16.978-7.9134
230.0703-0.31080.32932.8251-0.77642.73430.06140.1091-0.102-0.0994-0.2422-0.2310.14190.38930.14420.1761-0.00990.02940.26410.01920.256322.9143-30.64754.5456
240.20310.80490.68023.3067-0.4598.5414-0.2346-0.17440.05580.25550.0118-0.1937-0.17460.26820.26770.33550.07680.06240.31940.0350.374127.1651-41.9524-2.8168
250.8962-0.3291-0.26351.6770.13182.4340.02040.0756-0.0879-0.3202-0.09910.11790.1934-0.17990.07580.2856-0.00410.01950.2444-0.01770.295215.7563-38.0386-7.1797
264.36490.74221.38794.70370.06916.47010.1307-0.1939-0.3340.12730.02260.02450.4764-0.2627-0.16130.34630.0170.04770.23180.08290.302621.3232-46.617415.674
272.76320.6477-0.46050.5908-1.01593.1469-0.1471-0.0125-0.2955-0.4742-0.0125-0.20640.72980.1650.14920.32430.03180.01360.2248-0.00880.273117.7608-42.08960.7509
283.4085-4.424-0.16958.41034.11365.97350.00170.5156-0.19410.01290.06440.96410.0455-0.9122-0.27040.2862-0.01840.08510.36270.06240.440210.6469-23.986215.8702
290.6583-0.06260.53661.9238-0.25162.76650.005-0.0408-0.1646-0.1633-0.05860.25720.1757-0.20550.04180.22470.01370.01050.2272-0.02370.290810.6858-40.2159-0.7388
302.0545-2.1463-0.56065.812-1.08594.42540.30730.6615-0.2862-0.8946-0.2371-0.0540.07550.4865-0.0790.61660.12460.02530.3904-0.0210.279215.7196-26.1472-26.2464
314.3879-2.514-2.19267.5758-0.51922.9916-0.0733-1.0366-0.02980.8851-0.1952-0.71030.85540.50530.14060.38370.090.01740.37940.09290.383742.1919-65.864856.9348
322.18150.5073-0.41743.2920.91491.9577-0.20090.0914-0.1955-0.2630.04620.20270.1358-0.08540.15080.30410.01890.02390.23670.02420.231530.1901-60.329748.6819
331.41731.25922.23379.9823-0.58634.30260.31890.9271-1.5887-0.3863-0.4373-0.71990.98481.0120.2530.54160.10510.09590.4067-0.01870.536242.8368-67.351248.7775
340.9672-0.19762.11810.1139-0.43974.60680.3584-0.22760.0843-0.40710.1292-0.2625-0.0095-0.9764-0.18730.5899-0.02940.21940.5603-0.00340.779546.6464-70.060236.1046
353.09260.71541.03995.22864.25283.5339-0.1740.1834-0.5787-0.20820.0914-0.10330.1404-0.08560.16730.3978-0.02420.09480.3412-0.05920.385644.359-64.224432.1157
364.92391.9407-0.98662.67950.29052.0120.1444-0.0481-0.1872-0.0492-0.15590.3911-0.0293-0.28960.00020.26750.03350.00820.2196-0.04090.251627.3252-57.732949.0673
373.86661.0184-0.83992.27511.1128.3022-0.37860.14720.31630.3484-0.64420.865-0.442-1.65550.90130.27030.0737-0.01980.579-0.13340.619712.596-61.982449.4392
383.08961.8665-1.4466.3873-1.03543.30110.12380.00140.3850.1525-0.18830.6565-0.6099-0.33760.00350.33320.119-0.09390.4258-0.06770.481919.1511-43.431753.6955
396.33461.4394-1.91112.4948-0.18332.277-0.0278-0.11550.1871-0.18640.0120.0844-0.0991-0.10950.04170.29070.0331-0.00560.21470.01870.195535.8038-55.967244.2714
401.76911.154-1.18743.89421.22372.2882-0.0568-0.3219-0.06690.58370.091-0.32640.05790.5033-0.07880.33020.0439-0.03130.3986-0.00340.300940.9042-43.025458.5892
413.68321.89830.47623.57090.73993.33790.1046-0.5417-0.19550.538-0.1449-0.05060.2498-0.02650.02970.47870.05340.0760.40540.03910.311329.0825-57.443169.2713
422.23931.4016-0.47462.29970.27161.69420.0449-0.48080.4050.5415-0.0490.3047-0.09980.0760.0210.38540.08960.01290.3867-0.07980.307232.5059-38.050964.9128
436.54761.54141.84174.3504-0.23252.7011-0.51770.55130.40840.4669-0.36992.8809-0.1679-0.52680.9630.59380.0122-0.08560.4401-0.12050.771636.3749-29.421444.8125
441.82581.8647-0.45143.9771-1.23942.98970.0033-0.34310.19240.1406-0.11550.50610.133-0.06680.05970.31410.06180.02540.3383-0.05110.314325.2473-44.053562.2033
451.87130.3443-0.19686.84971.59181.9801-0.0868-0.0562-0.01660.41910.00690.45810.1511-0.15090.11720.28080.03620.03220.3370.0110.261926.2795-52.589656.0667
463.44383.20091.62465.87526.15548.23140.6896-1.678-0.8360.5601-0.4887-0.95522.20130.5789-0.21380.49190.0095-0.23820.49260.01220.669864.1956-41.696456.971
471.6783-0.7059-0.0511.6917-1.03732.7285-0.1602-0.25260.3526-0.13630.1715-0.4986-0.23560.2519-0.00530.2691-0.0643-0.02910.3374-0.09820.410257.8151-32.320245.051
481.9149-1.1757-1.19781.84291.24991.56120.181-0.40930.10080.4577-0.00560.0812-0.22390.3882-0.19020.5247-0.0942-0.08690.4459-0.1410.530955.6719-23.210757.2499
491.59980.5275-0.21322.85040.86231.96490.0111-0.2620.05180.30290.1267-0.2593-0.05360.0612-0.17930.4531-0.0521-0.03770.36-0.09520.362254.2977-33.100753.685
503.4461-2.6689-3.67474.73283.77588.2781-0.44991.31930.1578-1.28950.3019-0.1541-0.5071-1.19530.07070.7915-0.23590.02930.395-0.00940.6458.9758-16.978632.2784
517.49684.05821.384.05280.0153.8033-0.65160.4155-0.0027-1.55840.5720.0785-0.3525-0.07070.09460.7571-0.1205-0.04060.33520.02680.314748.7023-32.791524.8737
522.17690.2119-0.84934.29110.13013.1246-0.084-0.08290.1593-0.14170.1298-0.119-0.11570.1666-0.04420.2671-0.0131-0.05580.2697-0.04860.312149.0821-31.036647.0012
537.21521.1945-0.2974.10424.0325.4726-0.1168-0.2077-0.51510.0020.3631-0.60620.12990.5334-0.30830.31680.00810.03430.328-0.03010.370153.8666-54.767239.9506
541.79040.76680.17083.57090.85131.0073-0.16420.1874-0.1004-0.44340.2968-0.6919-0.07050.3398-0.12560.3873-0.07210.11970.3888-0.07250.380158.5845-46.362731.9558
552.01470.5777-0.39662.4660.96752.6017-0.15210.399-0.3323-0.62180.4268-0.51140.0330.4232-0.23240.4697-0.11140.12550.4075-0.13160.450559.4432-50.315728.5883
567.3223-4.0511-0.06289.7652-0.2832.0007-0.45770.3190.4318-0.63360.6759-0.0123-0.71860.63620.18560.5701-0.0395-0.09490.43410.00630.36333.5281-44.821835.806
573.38680.8285-0.62932.7814-0.38823.4643-0.31670.40380.1078-0.79470.3088-0.5722-0.24580.28570.010.5436-0.11370.11730.3199-0.05410.418257.8339-38.879328.5874
588.8193-4.0126-4.34562.15633.27547.87070.06120.50091.0977-1.22170.4388-0.1184-0.30360.0981-0.41250.7882-0.20190.03270.3995-0.01190.340347.0502-47.419222.0831
591.04640.65721.02412.49371.90711.615-0.17170.3073-0.2784-0.55310.5546-0.5229-0.34660.4108-0.38280.5279-0.1620.06730.4038-0.04250.436756.8333-31.200336.0181
608.6297-0.19491.13963.7393.09213.7972-0.0209-1.02051.15930.34280.3764-1.3843-0.02230.0075-0.30130.4582-0.0055-0.10540.3906-0.19040.804667.8399-21.244848.2096
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1(chain A and resid 56:60)A56 - 60
2X-RAY DIFFRACTION2(chain A and resid 61:86)A61 - 86
3X-RAY DIFFRACTION3(chain A and resid 87:109)A87 - 109
4X-RAY DIFFRACTION4(chain A and resid 110:117)A110 - 117
5X-RAY DIFFRACTION5(chain A and resid 118:133)A118 - 133
6X-RAY DIFFRACTION6(chain A and resid 134:158)A134 - 158
7X-RAY DIFFRACTION7(chain A and resid 159:165)A159 - 165
8X-RAY DIFFRACTION8(chain A and resid 166:181)A166 - 181
9X-RAY DIFFRACTION9(chain A and resid 182:239)A182 - 239
10X-RAY DIFFRACTION10(chain A and resid 240:276)A240 - 276
11X-RAY DIFFRACTION11(chain A and resid 277:296)A277 - 296
12X-RAY DIFFRACTION12(chain A and resid 297:345)A297 - 345
13X-RAY DIFFRACTION13(chain A and resid 346:355)A346 - 355
14X-RAY DIFFRACTION14(chain A and resid 356:378)A356 - 378
15X-RAY DIFFRACTION15(chain A and resid 379:414)A379 - 414
16X-RAY DIFFRACTION16(chain B and resid 56:61)B56 - 61
17X-RAY DIFFRACTION17(chain B and resid 62:96)B62 - 96
18X-RAY DIFFRACTION18(chain B and resid 97:110)B97 - 110
19X-RAY DIFFRACTION19(chain B and resid 111:125)B111 - 125
20X-RAY DIFFRACTION20(chain B and resid 126:158)B126 - 158
21X-RAY DIFFRACTION21(chain B and resid 159:187)B159 - 187
22X-RAY DIFFRACTION22(chain B and resid 188:214)B188 - 214
23X-RAY DIFFRACTION23(chain B and resid 215:238)B215 - 238
24X-RAY DIFFRACTION24(chain B and resid 239:248)B239 - 248
25X-RAY DIFFRACTION25(chain B and resid 249:286)B249 - 286
26X-RAY DIFFRACTION26(chain B and resid 287:325)B287 - 325
27X-RAY DIFFRACTION27(chain B and resid 326:345)B326 - 345
28X-RAY DIFFRACTION28(chain B and resid 346:357)B346 - 357
29X-RAY DIFFRACTION29(chain B and resid 358:401)B358 - 401
30X-RAY DIFFRACTION30(chain B and resid 402:414)B402 - 414
31X-RAY DIFFRACTION31(chain C and resid 56:63)C56 - 63
32X-RAY DIFFRACTION32(chain C and resid 64:103)C64 - 103
33X-RAY DIFFRACTION33(chain C and resid 104:110)C104 - 110
34X-RAY DIFFRACTION34(chain C and resid 111:117)C111 - 117
35X-RAY DIFFRACTION35(chain C and resid 118:130)C118 - 130
36X-RAY DIFFRACTION36(chain C and resid 131:154)C131 - 154
37X-RAY DIFFRACTION37(chain C and resid 155:162)C155 - 162
38X-RAY DIFFRACTION38(chain C and resid 163:184)C163 - 184
39X-RAY DIFFRACTION39(chain C and resid 185:214)C185 - 214
40X-RAY DIFFRACTION40(chain C and resid 215:239)C215 - 239
41X-RAY DIFFRACTION41(chain C and resid 240:265)C240 - 265
42X-RAY DIFFRACTION42(chain C and resid 266:345)C266 - 345
43X-RAY DIFFRACTION43(chain C and resid 346:354)C346 - 354
44X-RAY DIFFRACTION44(chain C and resid 355:381)C355 - 381
45X-RAY DIFFRACTION45(chain C and resid 382:414)C382 - 414
46X-RAY DIFFRACTION46(chain D and resid 56:60)D56 - 60
47X-RAY DIFFRACTION47(chain D and resid 61:88)D61 - 88
48X-RAY DIFFRACTION48(chain D and resid 89:122)D89 - 122
49X-RAY DIFFRACTION49(chain D and resid 123:146)D123 - 146
50X-RAY DIFFRACTION50(chain D and resid 147:165)D147 - 165
51X-RAY DIFFRACTION51(chain D and resid 166:179)D166 - 179
52X-RAY DIFFRACTION52(chain D and resid 180:221)D180 - 221
53X-RAY DIFFRACTION53(chain D and resid 222:240)D222 - 240
54X-RAY DIFFRACTION54(chain D and resid 241:309)D241 - 309
55X-RAY DIFFRACTION55(chain D and resid 310:345)D310 - 345
56X-RAY DIFFRACTION56(chain D and resid 346:355)D346 - 355
57X-RAY DIFFRACTION57(chain D and resid 356:379)D356 - 379
58X-RAY DIFFRACTION58(chain D and resid 380:390)D380 - 390
59X-RAY DIFFRACTION59(chain D and resid 391:403)D391 - 403
60X-RAY DIFFRACTION60(chain D and resid 404:414)D404 - 414

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