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Yorodumi- PDB-7peo: Structure of the Caulobacter crescentus S-layer protein RsaA N-te... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7peo | |||||||||
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Title | Structure of the Caulobacter crescentus S-layer protein RsaA N-terminal domain bound to LPS and soaked with Holmium | |||||||||
Components | S-layer protein | |||||||||
Keywords | STRUCTURAL PROTEIN / S-layer protein RsaA bound to LPS and Holmium | |||||||||
Function / homology | RsaA N-terminal domain / S-layer / RTX calcium-binding nonapeptide repeat / RTX calcium-binding nonapeptide repeat (4 copies) / Serralysin-like metalloprotease, C-terminal / calcium ion binding / extracellular region / HOLMIUM ATOM / S-layer protein Function and homology information | |||||||||
Biological species | Caulobacter vibrioides (bacteria) | |||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.37 Å | |||||||||
Authors | von Kugelgen, A. / Bharat, T.A.M. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: Structure / Year: 2022 Title: High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells. Authors: Matthew Herdman / Andriko von Kügelgen / Danguole Kureisaite-Ciziene / Ramona Duman / Kamel El Omari / Elspeth F Garman / Andreas Kjaer / Dimitrios Kolokouris / Jan Löwe / Armin Wagner / ...Authors: Matthew Herdman / Andriko von Kügelgen / Danguole Kureisaite-Ciziene / Ramona Duman / Kamel El Omari / Elspeth F Garman / Andreas Kjaer / Dimitrios Kolokouris / Jan Löwe / Armin Wagner / Phillip J Stansfeld / Tanmay A M Bharat / Abstract: Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in ...Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Utilizing optical microscopy of fluorescently tagged S-layers, we show that calcium ions facilitate S-layer lattice formation and cell-surface binding. We report all-atom molecular dynamics simulations of the S-layer lattice, revealing the importance of bound metal ions. Finally, using electron cryomicroscopy and long-wavelength X-ray diffraction experiments, we mapped the positions of metal ions in the S-layer at near-atomic resolution, supporting our insights from the cellular and simulations data. Our findings contribute to the understanding of how C. crescentus cells form a regularly arranged S-layer on their surface, with implications on fundamental S-layer biology and the synthetic biology of self-assembling biomaterials. | |||||||||
History |
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-Structure visualization
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Structure viewer | Molecule: MolmilJmol/JSmol |
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PDBx/mmCIF format | 7peo.cif.gz | 64.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7peo.ent.gz | 45.5 KB | Display | PDB format |
PDBx/mmJSON format | 7peo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pe/7peo ftp://data.pdbj.org/pub/pdb/validation_reports/pe/7peo | HTTPS FTP |
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-Related structure data
Related structure data | 13355MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | |
EM raw data | EMPIAR-10790 (Title: High-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells Data size: 304.4 Data #1: Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours (converted from .mrc into .tif with relion_convert_to_tiff) [micrographs - multiframe] Data #2: Unaligned multiframe micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt (converted from .mrc into .tif with relion_convert_to_tiff) [micrographs - multiframe] Data #3: Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours [micrographs - single frame] Data #4: Aligned and dose-weighted micrographs of RsaA_NTD spiral soaked with 5 mM HoCl3 for 2 hours with a 30 degree stage tilt [micrographs - single frame] Data #5: Gain reference image of the non-tilted and tilted dataset in MRC format [micrographs - single frame]) |
-Links
-Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
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