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TitleHigh-resolution mapping of metal ions reveals principles of surface layer assembly in Caulobacter crescentus cells.
Journal, issue, pagesStructure, Vol. 30, Issue 2, Page 215-228.e5, Year 2022
Publish dateFeb 3, 2022
AuthorsMatthew Herdman / Andriko von Kügelgen / Danguole Kureisaite-Ciziene / Ramona Duman / Kamel El Omari / Elspeth F Garman / Andreas Kjaer / Dimitrios Kolokouris / Jan Löwe / Armin Wagner / Phillip J Stansfeld / Tanmay A M Bharat /
PubMed AbstractSurface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in ...Surface layers (S-layers) are proteinaceous crystalline coats that constitute the outermost component of most prokaryotic cell envelopes. In this study, we have investigated the role of metal ions in the formation of the Caulobacter crescentus S-layer using high-resolution structural and cell biology techniques, as well as molecular simulations. Utilizing optical microscopy of fluorescently tagged S-layers, we show that calcium ions facilitate S-layer lattice formation and cell-surface binding. We report all-atom molecular dynamics simulations of the S-layer lattice, revealing the importance of bound metal ions. Finally, using electron cryomicroscopy and long-wavelength X-ray diffraction experiments, we mapped the positions of metal ions in the S-layer at near-atomic resolution, supporting our insights from the cellular and simulations data. Our findings contribute to the understanding of how C. crescentus cells form a regularly arranged S-layer on their surface, with implications on fundamental S-layer biology and the synthetic biology of self-assembling biomaterials.
External linksStructure / PubMed:34800371 / PubMed Central
MethodsEM (single particle)
Resolution4.37 Å
Structure data

13355

7peo

EMDB-13355, PDB-7peo:
Structure of the Caulobacter crescentus S-layer protein RsaA N-terminal domain bound to LPS and soaked with Holmium
Method: EM (single particle) / Resolution: 4.37 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HO:
HOLMIUM ATOM

Source
  • caulobacter vibrioides (bacteria)
KeywordsSTRUCTURAL PROTEIN / S-layer protein RsaA bound to LPS and Holmium

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