Journal: Nanoscale / Year: 2022 Title: Cryo-EM reconstructions of BMV-derived virus-like particles reveal assembly defects in the icosahedral lattice structure. Authors: Milosz Ruszkowski / Aleksander Strugala / Paulina Indyka / Guillaume Tresset / Marek Figlerowicz / Anna Urbanowicz / Abstract: The increasing interest in virus-like particles (VLPs) has been reflected by the growing number of studies on their assembly and application. However, the formation of complete VLPs is a complex ...The increasing interest in virus-like particles (VLPs) has been reflected by the growing number of studies on their assembly and application. However, the formation of complete VLPs is a complex phenomenon, making it difficult to rationally design VLPs with desired features . In this paper, we describe VLPs assembled from the recombinant capsid protein of brome mosaic virus (BMV). The analysis of VLPs was performed by Cryo-EM reconstructions and allowed us to visualize a few classes of VLPs, giving insight into the VLP self-assembly process. Apart from the mature icosahedral VLP practically identical with native virions, we describe putative VLP intermediates displaying non-icosahedral arrangements of capsomers, proposed to occur before the final disorder-order transition stage of icosahedral VLP assembly. Some of the described VLP classes show a lack of protein shell continuity, possibly resulting from too strong interaction with the cargo (in this case tRNA) with the capsid protein. We believe that our results are a useful prerequisite for the rational design of VLPs in the future and lead the way to the effective production of modified VLPs.
A: Coat protein B: Coat protein C: Coat protein D: Coat protein E: Coat protein F: Coat protein G: Coat protein H: Coat protein I: Coat protein J: Coat protein K: Coat protein L: Coat protein M: Coat protein N: Coat protein O: Coat protein P: Coat protein Q: Coat protein R: Coat protein S: Coat protein T: Coat protein V: Coat protein W: Coat protein X: Coat protein Y: Coat protein Z: Coat protein AA: Coat protein BA: Coat protein CA: Coat protein DA: Coat protein EA: Coat protein FA: Coat protein GA: Coat protein HA: Coat protein IA: Coat protein JA: Coat protein KA: Coat protein LA: Coat protein MA: Coat protein NA: Coat protein OA: Coat protein PA: Coat protein QA: Coat protein RA: Coat protein SA: Coat protein TA: Coat protein UA: Coat protein VA: Coat protein WA: Coat protein XA: Coat protein YA: Coat protein ZA: Coat protein AB: Coat protein BB: Coat protein CB: Coat protein DB: Coat protein EB: Coat protein FB: Coat protein GB: Coat protein HB: Coat protein IB: Coat protein JB: Coat protein KB: Coat protein LB: Coat protein MB: Coat protein NB: Coat protein OB: Coat protein PB: Coat protein QB: Coat protein RB: Coat protein SB: Coat protein TB: Coat protein UB: Coat protein VB: Coat protein WB: Coat protein XB: Coat protein YB: Coat protein ZB: Coat protein AC: Coat protein BC: Coat protein CC: Coat protein DC: Coat protein EC: Coat protein FC: Coat protein GC: Coat protein HC: Coat protein IC: Coat protein JC: Coat protein KC: Coat protein LC: Coat protein MC: Coat protein NC: Coat protein OC: Coat protein PC: Coat protein QC: Coat protein RC: Coat protein SC: Coat protein TC: Coat protein UC: Coat protein VC: Coat protein WC: Coat protein XC: Coat protein YC: Coat protein ZC: Coat protein AD: Coat protein BD: Coat protein CD: Coat protein DD: Coat protein ED: Coat protein FD: Coat protein GD: Coat protein HD: Coat protein ID: Coat protein JD: Coat protein KD: Coat protein LD: Coat protein MD: Coat protein ND: Coat protein OD: Coat protein PD: Coat protein QD: Coat protein RD: Coat protein SD: Coat protein TD: Coat protein UD: Coat protein VD: Coat protein WD: Coat protein XD: Coat protein YD: Coat protein ZD: Coat protein AE: Coat protein BE: Coat protein CE: Coat protein DE: Coat protein EE: Coat protein FE: Coat protein GE: Coat protein HE: Coat protein IE: Coat protein JE: Coat protein KE: Coat protein LE: Coat protein ME: Coat protein NE: Coat protein OE: Coat protein PE: Coat protein QE: Coat protein RE: Coat protein SE: Coat protein TE: Coat protein UE: Coat protein VE: Coat protein WE: Coat protein XE: Coat protein YE: Coat protein ZE: Coat protein AF: Coat protein BF: Coat protein CF: Coat protein DF: Coat protein EF: Coat protein FF: Coat protein GF: Coat protein HF: Coat protein IF: Coat protein JF: Coat protein KF: Coat protein LF: Coat protein MF: Coat protein NF: Coat protein OF: Coat protein PF: Coat protein QF: Coat protein RF: Coat protein SF: Coat protein TF: Coat protein UF: Coat protein VF: Coat protein WF: Coat protein XF: Coat protein YF: Coat protein
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VIRUS LIKE PARTICLE / BMV / 
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Mass: 18.015 Da / Num. of mol.: 180 / Source method: isolated from a natural source / Formula: H2O
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