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Open data
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Basic information
Entry | Database: PDB / ID: 7p8z | ||||||
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Title | Short wilavidin biotin complex | ||||||
![]() | Wilavidin | ||||||
![]() | UNKNOWN FUNCTION / Biotin binding protein / avidin / biotechnology | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Avraham, O. / Livnah, O. | ||||||
![]() | ![]() Title: Wilavidin - a novel member of the avidin family that forms unique biotin-binding hexamers. Authors: Avraham, O. / Bayer, E.A. / Livnah, O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 191.1 KB | Display | ![]() |
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PDB format | ![]() | 151.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.5 MB | Display | ![]() |
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Full document | ![]() | 1.5 MB | Display | |
Data in XML | ![]() | 18.9 KB | Display | |
Data in CIF | ![]() | 26.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7ouqSC ![]() 7ourC ![]() 7p8yC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 4 molecules ABCD
#1: Protein | Mass: 12786.995 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: short wilavidin Source: (gene. exp.) ![]() Gene: C4528_07355 / Production host: ![]() ![]() |
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-Non-polymers , 8 types, 167 molecules ![](data/chem/img/BTN.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/P33.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/PG4.gif)
![](data/chem/img/PGE.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/P33.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-BTN / #3: Chemical | ChemComp-PG4 / | #4: Chemical | ChemComp-PGE / | #5: Chemical | #6: Chemical | #7: Chemical | #8: Chemical | #9: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.29 Å3/Da / Density % sol: 46.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 1.5-1.7 M ammonium sulfate 0.1 M HEPES at pH 7.5 2-3% PEG 400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: May 8, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.96546 Å / Relative weight: 1 |
Reflection | Resolution: 1.666→77.79 Å / Num. obs: 55507 / % possible obs: 99.6 % / Redundancy: 6.5 % / CC1/2: 0.998 / Rmerge(I) obs: 0.106 / Net I/σ(I): 10.1 |
Reflection shell | Resolution: 1.666→1.695 Å / Num. unique obs: 2756 / CC1/2: 0.901 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7OUQ Resolution: 1.67→77.79 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.944 / SU B: 5.42 / SU ML: 0.081 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.137 / ESU R Free: 0.112 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 74.56 Å2 / Biso mean: 16.459 Å2 / Biso min: 5.52 Å2
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Refinement step | Cycle: final / Resolution: 1.67→77.79 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.67→1.709 Å / Rfactor Rfree error: 0
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