温度: 291.15 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 7.5 詳細: MtGlnK2 apo was crystallized at a concentration of 30 mg/ml in 25 mM Tris/HCl pH 7.6, 10% glycerol, 2mM dithiothreitol and 500 mM NaCl on a 96-Well MRC 2-Drop Crystallization Plates in ...詳細: MtGlnK2 apo was crystallized at a concentration of 30 mg/ml in 25 mM Tris/HCl pH 7.6, 10% glycerol, 2mM dithiothreitol and 500 mM NaCl on a 96-Well MRC 2-Drop Crystallization Plates in polystyrene (SWISSCI). Drop of 0.6 ul of protein was mixed with 0.6 ul of the crystallization solution. The reservoir contained 90 ul of the following crystallization solution 35 % w/v Pentaerythritol ethoxylate (15/4 EO/OH) and 100 mM HEPES pH 7.5. PH範囲: /
解像度: 2.3→73.16 Å / 交差検証法: THROUGHOUT / σ(F): 85.43 / 位相誤差: 42.05 / 立体化学のターゲット値: TWIN_LSQ_F 詳細: The refinement was performed with the following twin operator: k,h,-l
Rfactor
反射数
%反射
Rfree
0.2445
3625
5.31 %
Rwork
0.2215
66701
-
obs
0.233
70326
82.04 %
溶媒の処理
減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL