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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7ors | ||||||
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タイトル | Ternary complex of 14-3-3 sigma, p27pT198 phosphopeptide, and WQ147 | ||||||
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![]() | SIGNALING PROTEIN / protein-peptide complex small-molecule | ||||||
機能・相同性 | ![]() cyclin-dependent protein kinase activating kinase regulator activity / regulation of lens fiber cell differentiation / negative regulation of cardiac muscle tissue regeneration / negative regulation of cyclin-dependent protein kinase activity / autophagic cell death / FOXO-mediated transcription of cell cycle genes / negative regulation of epithelial cell proliferation involved in prostate gland development / regulation of cell cycle G1/S phase transition / regulation of exit from mitosis / epithelial cell proliferation involved in prostate gland development ...cyclin-dependent protein kinase activating kinase regulator activity / regulation of lens fiber cell differentiation / negative regulation of cardiac muscle tissue regeneration / negative regulation of cyclin-dependent protein kinase activity / autophagic cell death / FOXO-mediated transcription of cell cycle genes / negative regulation of epithelial cell proliferation involved in prostate gland development / regulation of cell cycle G1/S phase transition / regulation of exit from mitosis / epithelial cell proliferation involved in prostate gland development / negative regulation of epithelial cell apoptotic process / negative regulation of cyclin-dependent protein serine/threonine kinase activity / ubiquitin ligase activator activity / negative regulation of phosphorylation / cyclin-dependent protein serine/threonine kinase inhibitor activity / RHO GTPases activate CIT / nuclear export / AKT phosphorylates targets in the cytosol / Cul4A-RING E3 ubiquitin ligase complex / epithelial cell apoptotic process / cellular response to antibiotic / negative regulation of kinase activity / regulation of cyclin-dependent protein serine/threonine kinase activity / molecular function inhibitor activity / cellular response to lithium ion / regulation of epidermal cell division / protein kinase C inhibitor activity / protein kinase inhibitor activity / positive regulation of epidermal cell differentiation / keratinocyte development / keratinization / p53-Dependent G1 DNA Damage Response / PTK6 Regulates Cell Cycle / Constitutive Signaling by AKT1 E17K in Cancer / regulation of G1/S transition of mitotic cell cycle / negative regulation of vascular associated smooth muscle cell proliferation / Defective binding of RB1 mutants to E2F1,(E2F2, E2F3) / inner ear development / cellular response to organic cyclic compound / Regulation of localization of FOXO transcription factors / keratinocyte proliferation / negative regulation of mitotic cell cycle / Estrogen-dependent nuclear events downstream of ESR-membrane signaling / phosphoserine residue binding / Activation of BAD and translocation to mitochondria / localization / negative regulation of keratinocyte proliferation / response to amino acid / establishment of skin barrier / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / TP53 Regulates Transcription of Genes Involved in G1 Cell Cycle Arrest / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / response to glucose / Cyclin E associated events during G1/S transition / response to cadmium ion / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / negative regulation of stem cell proliferation / Cyclin A:Cdk2-associated events at S phase entry / RHO GTPases activate PKNs / Notch signaling pathway / regulation of cell migration / positive regulation of microtubule polymerization / protein kinase A signaling / protein sequestering activity / Hsp70 protein binding / DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest / FLT3 Signaling / negative regulation of innate immune response / protein export from nucleus / cyclin binding / TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest / positive regulation of protein export from nucleus / release of cytochrome c from mitochondria / positive regulation of DNA replication / stem cell proliferation / Translocation of SLC2A4 (GLUT4) to the plasma membrane / TP53 Regulates Metabolic Genes / sensory perception of sound / negative regulation of protein kinase activity / potassium ion transport / negative regulation of cysteine-type endopeptidase activity involved in apoptotic process / placenta development / DNA Damage/Telomere Stress Induced Senescence / negative regulation of cell growth / SCF(Skp2)-mediated degradation of p27/p21 / response to peptide hormone / G1/S transition of mitotic cell cycle / positive regulation of protein catabolic process / Cyclin D associated events in G1 / intrinsic apoptotic signaling pathway in response to DNA damage / cellular senescence / response to estradiol / heart development / protein-folding chaperone binding / cellular response to hypoxia / Senescence-Associated Secretory Phenotype (SASP) / positive regulation of cell growth / protein phosphatase binding / molecular adaptor activity / regulation of cell cycle 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Centorrino, F. / Wu, Q. / Ottmann, C. | ||||||
資金援助 | European Union, 1件
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![]() | ![]() タイトル: A crystallography-based study of fragment extensions into the 14-3-3 binding groove 著者: Centorrino, F. / Wu, Q. / Cossar, P. / Brunsveld, L. / Ottmann, C. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 139.9 KB | 表示 | ![]() |
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PDB形式 | ![]() | 88.4 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 752.2 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 753.8 KB | 表示 | |
XML形式データ | ![]() | 15.4 KB | 表示 | |
CIF形式データ | ![]() | 24 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 7opwC ![]() 7oq7C ![]() 7oq8C ![]() 7oq9C ![]() 7oqaC ![]() 7oqgC ![]() 7oqjC ![]() 7oqsC ![]() 7oquC ![]() 7oqwC ![]() 7or3C ![]() 7or5C ![]() 7or7C ![]() 7or8C ![]() 7orgC ![]() 7orhC ![]() 7ortC ![]() 4jc3S S: 精密化の開始モデル C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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Components on special symmetry positions |
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要素
-タンパク質 / タンパク質・ペプチド , 2種, 2分子 AP
#1: タンパク質 | 分子量: 28226.518 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() |
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#2: タンパク質・ペプチド | 分子量: 1522.712 Da / 分子数: 1 / 由来タイプ: 合成 / 由来: (合成) ![]() |
-非ポリマー , 4種, 379分子 ![](data/chem/img/MG.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/0I2.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/0I2.gif)
![](data/chem/img/HOH.gif)
#3: 化合物 | #4: 化合物 | ChemComp-CL / | #5: 化合物 | ChemComp-0I2 / ( | #6: 水 | ChemComp-HOH / | |
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-詳細
研究の焦点であるリガンドがあるか | Y |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.43 Å3/Da / 溶媒含有率: 49.48 % |
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結晶化 | 温度: 277.15 K / 手法: 蒸気拡散法, シッティングドロップ法 詳細: 0.095 M Hepes pH7.3, 26%PEG 400, 0.19 M CaCl2, and 5 % Glycerol |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: SEALED TUBE / タイプ: RIGAKU MICROMAX-003 / 波長: 1.54187 Å |
検出器 | タイプ: DECTRIS PILATUS 200K / 検出器: PIXEL / 日付: 2021年2月22日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.54187 Å / 相対比: 1 |
反射 | 解像度: 1.8→41.74 Å / Num. obs: 26844 / % possible obs: 98.5 % / 冗長度: 6.2 % / Biso Wilson estimate: 10.49 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.061 / Net I/σ(I): 19 |
反射 シェル | 解像度: 1.8→1.83 Å / 冗長度: 5.1 % / Rmerge(I) obs: 0.188 / Mean I/σ(I) obs: 6.3 / Num. unique obs: 1252 / CC1/2: 0.974 / % possible all: 93.4 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: 4jc3 解像度: 1.8→41.74 Å / SU ML: 0.1476 / 交差検証法: FREE R-VALUE / σ(F): 1.34 / 位相誤差: 16.9916 / 立体化学のターゲット値: GeoStd + Monomer Library
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溶媒の処理 | 減衰半径: 0.9 Å / VDWプローブ半径: 1.11 Å / 溶媒モデル: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 14.01 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.8→41.74 Å
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拘束条件 |
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LS精密化 シェル |
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