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Basic information
Entry | Database: PDB / ID: 7op2 | ||||||
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Title | Chadox1/ Chimpanzee adenovirus Y25 fiber knob protein | ||||||
![]() | Fiber | ||||||
![]() | VIRAL PROTEIN / Fiber knob / adenovirus / fiber protein / spike / fiber / chimpanzee adenovirus / simian adenovirus / ChAdOx1 / ChAd-Y25 / Y25 / Chimp Ad / ChAdV-Y25 / cell binding / CAR | ||||||
Function / homology | ![]() adhesion receptor-mediated virion attachment to host cell / viral capsid / cell adhesion / symbiont entry into host cell / host cell nucleus Similarity search - Function | ||||||
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![]() | Rizkallah, P.J. / Baker, A.T. / Parker, A.L. / Teijeira Crespo, A. / Lipka-Lloyd, M. | ||||||
![]() | ![]() Title: ChAdOx1 interacts with CAR and PF4 with implications for thrombosis with thrombocytopenia syndrome. Authors: Alexander T Baker / Ryan J Boyd / Daipayan Sarkar / Alicia Teijeira-Crespo / Chun Kit Chan / Emily Bates / Kasim Waraich / John Vant / Eric Wilson / Chloe D Truong / Magdalena Lipka-Lloyd / ...Authors: Alexander T Baker / Ryan J Boyd / Daipayan Sarkar / Alicia Teijeira-Crespo / Chun Kit Chan / Emily Bates / Kasim Waraich / John Vant / Eric Wilson / Chloe D Truong / Magdalena Lipka-Lloyd / Petra Fromme / Josh Vermaas / Dewight Williams / LeeAnn Machiesky / Meike Heurich / Bolni M Nagalo / Lynda Coughlan / Scott Umlauf / Po-Lin Chiu / Pierre J Rizkallah / Taylor S Cohen / Alan L Parker / Abhishek Singharoy / Mitesh J Borad / ![]() ![]() Abstract: Vaccines derived from chimpanzee adenovirus Y25 (ChAdOx1), human adenovirus type 26 (HAdV-D26), and human adenovirus type 5 (HAdV-C5) are critical in combatting the severe acute respiratory ...Vaccines derived from chimpanzee adenovirus Y25 (ChAdOx1), human adenovirus type 26 (HAdV-D26), and human adenovirus type 5 (HAdV-C5) are critical in combatting the severe acute respiratory coronavirus 2 (SARS-CoV-2) pandemic. As part of the largest vaccination campaign in history, ultrarare side effects not seen in phase 3 trials, including thrombosis with thrombocytopenia syndrome (TTS), a rare condition resembling heparin-induced thrombocytopenia (HIT), have been observed. This study demonstrates that all three adenoviruses deployed as vaccination vectors versus SARS-CoV-2 bind to platelet factor 4 (PF4), a protein implicated in the pathogenesis of HIT. We have determined the structure of the ChAdOx1 viral vector and used it in state-of-the-art computational simulations to demonstrate an electrostatic interaction mechanism with PF4, which was confirmed experimentally by surface plasmon resonance. These data confirm that PF4 is capable of forming stable complexes with clinically relevant adenoviruses, an important step in unraveling the mechanisms underlying TTS. #1: ![]() Title: The Structure of ChAdOx1/AZD-1222 Reveals Interactions with CAR and PF4 with Implications for Vaccine-induced Immune Thrombotic Thrombocytopenia Authors: Baker, A.T. / Boyd, R.J. / Sarkar, D. / Vant, J. / Crespo, A.T. / Truong, C.D. / Bates, E. / Wilson, E. / Chan, C.K. / Lipka-Lloyd, M. / Fromme, P. / Nagalo, M.B. / Heurich, M. / Williams, D. ...Authors: Baker, A.T. / Boyd, R.J. / Sarkar, D. / Vant, J. / Crespo, A.T. / Truong, C.D. / Bates, E. / Wilson, E. / Chan, C.K. / Lipka-Lloyd, M. / Fromme, P. / Nagalo, M.B. / Heurich, M. / Williams, D. / Chiu, P.L. / Rizkallah, P.J. / Parker, A.L. / Singharoy, A. / Borad, M.J. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 875.7 KB | Display | ![]() |
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PDB format | ![]() | 733 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 556.7 KB | Display | ![]() |
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Full document | ![]() | 593.4 KB | Display | |
Data in XML | ![]() | 93 KB | Display | |
Data in CIF | ![]() | 130.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7rd1C ![]() 1knbS S: Starting model for refinement C: citing same article ( |
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Assembly
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Noncrystallographic symmetry (NCS) | NCS domain:
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