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Open data
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Basic information
Entry | Database: PDB / ID: 7ofq | ||||||
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Title | The archaellum of Methanocaldococcus villosus | ||||||
![]() | (Archaellin) x 2 | ||||||
![]() | PROTEIN FIBRIL / Archaellum / archaellin / Methanocaldococcus villosus / cryoEM / helical reconstruction | ||||||
Function / homology | Unknown ligand![]() | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.08 Å | ||||||
![]() | Isupov, M. / Gambelli, L. / Daum, B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: An archaellum filament composed of two alternating subunits. Authors: Lavinia Gambelli / Michail N Isupov / Rebecca Conners / Mathew McLaren / Annett Bellack / Vicki Gold / Reinhard Rachel / Bertram Daum / ![]() ![]() Abstract: Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of ...Archaea use a molecular machine, called the archaellum, to swim. The archaellum consists of an ATP-powered intracellular motor that drives the rotation of an extracellular filament composed of multiple copies of proteins named archaellins. In many species, several archaellin homologs are encoded in the same operon; however, previous structural studies indicated that archaellum filaments mainly consist of only one protein species. Here, we use electron cryo-microscopy to elucidate the structure of the archaellum from Methanocaldococcus villosus at 3.08 Å resolution. The filament is composed of two alternating archaellins, suggesting that the architecture and assembly of archaella is more complex than previously thought. Moreover, we identify structural elements that may contribute to the filament's flexibility. #1: ![]() Title: Escaping the symmetry trap in helical reconstruction Authors: Gambelli, L. / Isupov, M.N. / Daum, B. | ||||||
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Structure visualization
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.9 MB | Display | ![]() |
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PDB format | ![]() | 1.6 MB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 959.8 KB | Display | ![]() |
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Full document | ![]() | 969.1 KB | Display | |
Data in XML | ![]() | 194.6 KB | Display | |
Data in CIF | ![]() | 295.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 12875MC M: map data used to model this data C: citing same article ( |
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Similar structure data | |
EM raw data | ![]() Data size: 3.3 TB Data #1: Unaligned 39-frames movies of Methanocaldococcus villosus archaella [micrographs - multiframe]) |
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Assembly
Deposited unit | ![]()
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Noncrystallographic symmetry (NCS) | NCS domain:
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