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- PDB-7o06: Crystal structure of the N-terminal domain of CEP164(1-109) bound... -

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Basic information

Entry
Database: PDB / ID: 7o06
TitleCrystal structure of the N-terminal domain of CEP164(1-109) bound to camelid nanobody 10Z
Components
  • Camelid nanobody 10Z
  • Centrosomal protein of 164 kDa
KeywordsSTRUCTURAL PROTEIN / Centriole / Centrosome / Basal body / Ciliogenesis / Cell cycle
Function / homology
Function and homology information


ciliary transition fiber / cilium assembly / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / centriole / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Regulation of PLK1 Activity at G2/M Transition ...ciliary transition fiber / cilium assembly / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / centriole / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / Regulation of PLK1 Activity at G2/M Transition / cell division / DNA repair / intracellular membrane-bounded organelle / centrosome / extracellular space / nucleoplasm / cytosol
Similarity search - Function
: / Photosystem 1 Reaction Centre Subunit Ii; Chain: D; / Photosystem I PsaD, reaction center subunit II / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain / Immunoglobulins / Immunoglobulin-like / Sandwich ...: / Photosystem 1 Reaction Centre Subunit Ii; Chain: D; / Photosystem I PsaD, reaction center subunit II / WW domain superfamily / WW/rsp5/WWP domain profile. / Domain with 2 conserved Trp (W) residues / WW domain / Immunoglobulins / Immunoglobulin-like / Sandwich / 2-Layer Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
Centrosomal protein of 164 kDa
Similarity search - Component
Biological speciesCamelidae mixed library (mammal)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.6 Å
Authorse Silva, I.R. / van Breugel, M.
Funding support United Kingdom, 1items
OrganizationGrant numberCountry
Medical Research Council (MRC, United Kingdom)MC_UP_1201/3 United Kingdom
CitationJournal: Structure / Year: 2022
Title: Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.
Authors: Rosa E Silva, I. / Bino, L. / Johnson, C.M. / Rutherford, T.J. / Neuhaus, D. / Andreeva, A. / Cajanek, L. / van Breugel, M.
History
DepositionMar 25, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 8, 2021Provider: repository / Type: Initial release
Revision 1.1Sep 15, 2021Group: Database references / Category: citation
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.title / _citation.year
Revision 1.2Sep 22, 2021Group: Data collection / Database references
Category: citation / citation_author ...citation / citation_author / diffrn_source / pdbx_database_proc
Item: _citation.pdbx_database_id_PubMed / _citation.title / _diffrn_source.pdbx_synchrotron_site
Revision 1.3Jan 19, 2022Group: Data collection / Database references / Category: citation / diffrn_source
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.year / _diffrn_source.pdbx_synchrotron_site
Revision 1.4Dec 21, 2022Group: Database references / Category: citation / Item: _citation.page_last
Revision 1.5Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Camelid nanobody 10Z
B: Camelid nanobody 10Z
C: Centrosomal protein of 164 kDa
D: Centrosomal protein of 164 kDa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)54,0445
Polymers53,9484
Non-polymers961
Water4,954275
1
A: Camelid nanobody 10Z
C: Centrosomal protein of 164 kDa
hetero molecules


Theoretical massNumber of molelcules
Total (without water)27,0703
Polymers26,9742
Non-polymers961
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2740 Å2
ΔGint-20 kcal/mol
Surface area10890 Å2
MethodPISA
2
B: Camelid nanobody 10Z
D: Centrosomal protein of 164 kDa


Theoretical massNumber of molelcules
Total (without water)26,9742
Polymers26,9742
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2580 Å2
ΔGint-9 kcal/mol
Surface area10880 Å2
MethodPISA
Unit cell
Length a, b, c (Å)50.852, 70.734, 61.969
Angle α, β, γ (deg.)90.000, 90.090, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

#1: Antibody Camelid nanobody 10Z


Mass: 14023.665 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Camelidae mixed library (mammal) / Production host: Escherichia coli (E. coli)
#2: Protein Centrosomal protein of 164 kDa / Cep164


Mass: 12950.511 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CEP164, KIAA1052, NPHP15 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9UPV0
#3: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: SO4 / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 275 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.07 Å3/Da / Density % sol: 40.46 %
Crystal growTemperature: 292 K / Method: vapor diffusion / Details: 0.1 M Hepes, pH 7.5, 1.26 M ammonium sulfate

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.97942 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 4, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97942 Å / Relative weight: 1
ReflectionResolution: 1.6→61.97 Å / Num. obs: 57658 / % possible obs: 99.4 % / Redundancy: 6.7 % / Biso Wilson estimate: 19.2 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.097 / Rpim(I) all: 0.04 / Net I/σ(I): 9.9
Reflection shellResolution: 1.6→1.63 Å / Redundancy: 6.3 % / Rmerge(I) obs: 1.463 / Mean I/σ(I) obs: 1.7 / Num. unique obs: 2820 / CC1/2: 0.649 / Rpim(I) all: 0.627 / % possible all: 98.9

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Processing

Software
NameVersionClassification
REFMAC5.7.0009refinement
PHENIX1.18_3845refinement
DIALSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5VLV

5vlv


Resolution: 1.6→61.97 Å / SU ML: 0.1781 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 24.7707
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2108 2843 4.95 %
Rwork0.1804 54611 -
obs0.1819 57454 99.12 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 28.06 Å2
Refinement stepCycle: LAST / Resolution: 1.6→61.97 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3396 0 5 275 3676
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00583617
X-RAY DIFFRACTIONf_angle_d0.79674926
X-RAY DIFFRACTIONf_chiral_restr0.0507511
X-RAY DIFFRACTIONf_plane_restr0.0048657
X-RAY DIFFRACTIONf_dihedral_angle_d20.0903519
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.6-1.630.30581790.27652646X-RAY DIFFRACTION97.95
1.63-1.660.29031460.26572658X-RAY DIFFRACTION97.6
1.66-1.690.25641280.24422690X-RAY DIFFRACTION97.98
1.69-1.720.2721420.24052705X-RAY DIFFRACTION98.27
1.72-1.760.29651390.23152700X-RAY DIFFRACTION98.37
1.76-1.80.25641180.21942721X-RAY DIFFRACTION98.47
1.8-1.850.25181320.21012738X-RAY DIFFRACTION98.56
1.85-1.90.28361440.19952688X-RAY DIFFRACTION98.74
1.9-1.950.20861420.19352738X-RAY DIFFRACTION99.11
1.95-2.020.26611280.18242715X-RAY DIFFRACTION99.16
2.02-2.090.2191520.18982728X-RAY DIFFRACTION99.38
2.09-2.170.20671440.18282734X-RAY DIFFRACTION99.58
2.17-2.270.22691370.18952737X-RAY DIFFRACTION99.62
2.27-2.390.23281810.18822722X-RAY DIFFRACTION99.83
2.39-2.540.20171410.18862770X-RAY DIFFRACTION99.93
2.54-2.740.21381140.1892766X-RAY DIFFRACTION99.9
2.74-3.010.21971450.18692762X-RAY DIFFRACTION100
3.01-3.450.20181190.17452809X-RAY DIFFRACTION100
3.45-4.340.15971550.14132756X-RAY DIFFRACTION100
4.34-61.970.18471570.15632828X-RAY DIFFRACTION99.87
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.505949328760.486801547197-3.852316735395.0596644516-0.05434774105514.305776606220.3863492371920.2254697119671.07678658536-0.5938950478830.531606045829-0.833272376852-0.8389785675670.61793487232-0.9929543952810.382592698431-0.03385134059640.05809912134720.334262884366-0.01051132801610.4472386858616.9329495647-0.50261949375-21.8622192264
24.586314298370.4922662951140.173650346164.40770208604-4.189153765399.4014093788-0.142319922422-0.1995311287940.3228638493670.1156408922110.11654244027-0.00700779900705-0.288437607879-0.3261844003760.01581328299660.1964130736090.04654159979560.002008121260070.192299427394-0.04334184112020.2139770765988.76065113478-2.75993081024-9.14910732559
35.70892889596-1.951874390854.495421453523.36321035186-0.6219027462925.141367251980.03958372673220.4073825250040.264146951278-0.229133930712-0.154934223994-0.0736211185283-0.1499669409590.2834261123770.2238841293050.132282403868-0.01538201079820.02375800584690.2135663079140.02868968098920.17783910619313.0319454309-9.05909450444-22.2267703728
42.275870406521.475682900312.12673445040.953430307971.489422880017.463875487090.1171204008460.0680014758444-0.1591648755970.0467600713650.0547183233301-0.1077039998140.1829772242860.32014083771-0.2245012705640.1313761492650.008365657464480.0004435911502520.1616108587640.0004472434527920.16219886496517.8566758187-14.9067868735-12.0109157079
56.20395014867-0.935869890543.528366791193.85288352785-2.599851285923.155184169340.172236859614-0.0073979357713-0.445294012468-0.1881905920870.00344658299525-0.00636581736280.862084195764-0.50062670811-0.2136595914030.180043400167-0.00856033447767-0.008831517604570.196745151802-0.002947319426040.2357704299046.83896082522-17.7255646582-18.4669473494
64.007246578271.0087221335-0.7851492852054.26747901727-3.193779267365.558168795880.0005157774717170.01422800496260.0995183233060.06130271776920.1886780092170.106360152349-0.178581681348-0.295813926059-0.2390351403130.1187951321040.02490781802810.002007843422630.1927048337680.002216536532820.161003523185.033301798-7.44774071353-16.9263579514
70.802874985853-0.3094883492661.295844128691.010265884071.167832416565.177478561030.0551703696878-0.180809622398-0.0675733864510.04314748475580.05657255701080.032741962283-0.327125348469-0.198170160604-0.1197469120970.114098702313-0.00459530451709-0.02602924221280.1960166743550.003377161878230.14775247269313.5693107506-10.4375668343-11.3993622014
84.6328795745-2.88851061746-0.9657357831365.032263598231.634415161675.124722570490.2172750683970.0444890037997-0.481871789069-0.153567137817-0.2280310244570.3532882349110.425756405249-0.3219839612690.03350062258110.165197321745-0.0461675684786-0.005350897090970.1597974019140.01736134651860.20113454328618.1385511721-13.7362293845-31.9392837757
99.08348335311-2.26213134796-5.850551637772.679068771521.923721814483.825377679880.181756819151-0.8367956825590.4843749487430.2256630125180.1910035620440.0285078511805-0.9628193704410.744058005476-0.4408703995670.242901527694-0.0313171124322-0.01485805053090.273835294056-0.06093408546580.19068245085315.4838048312-3.79406331342-3.5501498536
102.804085357042.86499673251-2.809710987728.31282725324-2.173777600122.931203835550.212357408853-0.5889069040180.1869846946990.799875771395-0.01368528612410.351795595889-0.793905038958-0.945801388007-0.3219146001010.3177947365650.0338683394991-0.0008030128611420.3904101140560.006068413170540.283266273216-16.2500826202-2.27494256751-7.54877715269
117.24306225926-4.454065256220.4046233775697.584293875653.196746402352.46559955350.1975692579230.7728688275480.554241404525-0.178813756187-0.452621671217-0.252587835469-0.4913833660550.4200032709960.2507554513510.251070676529-0.02512554876290.01259272753810.2640422160350.09607464102640.25159237916-7.706679388960.53385017096-28.7994957397
121.69794008563-0.5725658636182.071173558748.4439919438-4.360631149636.731503705240.0626245788758-0.472611591618-0.01475201129751.00453655556-0.1158429519250.0836310354956-0.8528094003110.1306879610340.0149492531270.190039474176-0.0279914405489-0.02222570921230.275062706563-0.0291642736160.179134664115-8.75455542034-6.21732834384-7.84941620738
135.05276277484-1.447563707274.947487274160.951501498068-1.606459822216.213623261450.0693767347269-0.0742781104198-0.178425651683-0.007614642716610.02802336641920.08042703619840.0603528093284-0.0670230247935-0.1265352637690.142380424459-0.004192518823560.009259806439370.1530006346850.008761334935510.171149697809-15.8354903042-12.5444498004-19.1922068747
144.69290159888-2.746064329853.697338586773.59511204943-3.191164937553.43577660589-0.0528180389149-0.236777216683-0.328702564469-0.0137443242316-0.0564331960159-0.2025723232490.5168917611030.5659456820440.001863763291660.1641827815890.00168294142557-0.007557576874950.219581688480.01407007123620.220670578856-5.00973420564-18.5424968123-12.0084890136
155.263879132230.3711207396632.497781877592.432859564370.0625693486939.924647643850.08343514932140.121352754581-0.292846304936-0.123960876533-0.0647801749831-0.01434045472160.1219513973470.232498481925-0.05857293152020.1072195630280.02406053740590.004331770137040.1266209687390.01165174037170.162645365019-4.90858629518-12.8188316595-18.8562991576
162.7792352583-1.51111807561-0.9981209653262.59107415976-0.8730723182785.99634823622-0.00904597998376-0.01425320221370.1638306625430.2164815860840.0885566590093-0.0752088478308-0.2059525188120.239928124257-0.08918250094370.171391658849-0.0482210387855-0.006702713131840.219907033313-0.01125160791410.187412683127-4.85810294308-5.40953032991-10.9223264731
171.0607557308-0.4003112575811.067155475161.58082057152-2.450111869673.961411359020.0900535778060.1771311868420.02728594042670.05513676419770.05678001406640.029984170267-0.06184001675930.0220272594954-0.1412023806460.1546478307660.00174224949856-0.01701284964630.152499514524-0.00377641608840.163412145737-12.8698668734-8.99438411149-20.3804828886
184.797619702511.58873360410.6829387219485.85928366402-2.81713097579.434822632610.0243945667091-0.176576141377-0.5702038941450.198186949462-0.0749054331155-0.3209139597970.4335730903250.1976245892990.01050127855080.1228581020970.02678096160940.02148942379060.1483802520490.010169723270.190915626436-17.4341750194-19.2576534395-2.48528651693
198.470897432923.00823304508-4.838963901988.26472141011-4.838685343374.058784801210.2162938352070.424063856050.4348768586230.07989153510920.1203830779960.157071296965-1.06684669334-0.380493379436-0.3812515491390.1836140125450.0248529545942-0.02708988086090.1823542443630.02619001006610.185716105529-14.7621121905-0.185898120363-25.3825546095
208.832957001363.95355361138-1.503986968914.64770827677-1.616335338423.63152286816-0.2392131033740.3186676699840.364920014562-0.2310282333270.6466167206950.7983374758950.32763956779-0.993270330415-0.4085242778930.239228616089-0.0185005542269-0.03510532415370.363771809935-0.0617440084470.28940715489812.0049928514-14.8414688925-32.8120433579
215.77100020307-0.889818825833-3.355132372163.997069278445.759525370349.07518282662-0.1287582046180.186789496260.2242554325420.287145701296-0.08880356968460.107953540328-0.382062556985-0.02142197785910.2041541182850.223866401869-0.0249551566461-0.04184806280990.2230244355210.03324994264840.21485699994327.646410325-5.43136882571-35.7866985974
225.686926088681.049644468940.2556545220294.02372269892-1.881477023426.96739530729-0.2025819868530.3130750066910.549369365070.14816536372-0.0371236676243-0.13199094624-0.7785570540860.3928053031240.2336402288430.26656696679-0.00261463418247-0.03593179311730.2010823658250.03587944091620.19836345540831.4822249632-6.16487153195-43.1599469226
232.21579695764-0.620953117505-2.116612166951.79245209963-1.457202277894.75659480006-0.04732444249680.0479862391979-0.220987722343-0.2628207094010.0156331082935-0.00230553158080.525088531373-0.190189798514-0.005806418464820.176165424141-0.0216903695965-0.006425956443480.127505434168-0.01001543763940.1714677383620.8679543961-22.6571994049-29.4125616133
242.224320410263.942877395490.2268579479547.8338344812-0.1993461292530.5174093863750.1218581388290.12645021981-0.1917054709890.309655711690.00147371729191-0.3275604625220.1643270565640.0226558609415-0.1042668754170.2265494535190.013272617232-0.01461245180580.21576443354-0.006464038872970.30914438696325.9552168031-25.7432193174-31.6816299011
256.100125047433.72855954876-5.453374122572.28703541531-3.211858239719.21793230294-0.03457591076770.127685273334-0.155473281086-0.0969516010326-0.139241503133-0.2107215301340.02802520577330.7205688184630.2726430911650.2107727309680.0269921090322-0.0001680940610580.340229116362-0.01462518528270.23805361105136.938094756-13.7563412696-45.0909516593
264.375929823162.284116016074.853726121788.176991382043.191912712255.444560288940.02852880077550.0674299536894-0.153889272455-0.1883346714220.0901035990304-0.6078875025110.9030738883081.15255223083-0.08703136406940.2982599615380.04601622972350.003627091464160.405083635581-0.01904350042920.349087265307-10.8097930141-23.7572283923-7.14240479518
276.23227970279-1.15456576841-1.927809313316.329970714581.47514796025.00075387694-0.052668200544-0.4473641086960.09119375526210.2415642576810.23180143486-0.8157681788360.03995970585141.13435522502-0.0821123138480.1884278347390.00420902387658-0.0001133435332230.3701806225230.00733564589670.261593125908-13.3544823151-15.67064401946.86872000684
282.069070180751.09672532014-0.6168858466843.309993583610.8226897187744.33788857327-0.2172709622850.223188811290.379800284782-0.3624189949050.0003906131046140.319003148639-0.535685586178-0.1844963825820.08751459412310.2552439336130.0479019548369-0.03169162350850.265726919813-0.003713891243390.218534589773-27.2924162019-12.70102172484.08677438252
298.667396028590.188404372017-1.230492903064.40265525090.8727580549385.96420357131-0.262914061705-0.400727535745-0.2618440281440.7178743571920.001978325722340.286978322317-0.0942578252235-0.5198046225520.1952406294460.294791665290.0149175581621-0.0003870745575910.2479791856150.008125428615070.195364698149-31.8734441886-16.239962042710.7047426555
300.2703562759150.061421045825-0.5812708311453.53518321853-1.938701573343.66270990296-0.11557620312-0.0447852842889-0.165631973158-0.2183558732470.1908875564840.4126752403420.491159850335-0.119994743544-0.1107336471030.202393481302-0.00511109685997-0.007757688700470.1811815570230.03250749185560.311759507399-22.12450879-27.8746843874-7.15245428698
315.60112926061-1.77891014264-4.236731984069.51098842518-0.3119131242398.9678970092-0.239359600439-0.108985880037-0.7068864592620.7081178631110.02113729352930.5176737934920.582281719153-0.6833708159810.2452684491190.318467480657-0.08292670092590.05586470807250.309412546320.03893929214120.444295566022-36.4758440414-24.31820685118.71834575098
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 8 through 14 )AA8 - 141 - 7
22chain 'A' and (resid 15 through 32 )AA15 - 328 - 31
33chain 'A' and (resid 33 through 46 )AA33 - 4632 - 48
44chain 'A' and (resid 47 through 58 )AA47 - 5849 - 62
55chain 'A' and (resid 59 through 70 )AA59 - 7063 - 77
66chain 'A' and (resid 71 through 89 )AA71 - 8978 - 97
77chain 'A' and (resid 90 through 107 )AA90 - 10798 - 115
88chain 'A' and (resid 108 through 119 )AA108 - 119116 - 129
99chain 'A' and (resid 120 through 131 )AA120 - 131130 - 141
1010chain 'B' and (resid 8 through 14 )BC8 - 141 - 8
1111chain 'B' and (resid 15 through 24 )BC15 - 249 - 18
1212chain 'B' and (resid 25 through 39 )BC25 - 3919 - 38
1313chain 'B' and (resid 40 through 58 )BC40 - 5839 - 59
1414chain 'B' and (resid 59 through 66 )BC59 - 6660 - 69
1515chain 'B' and (resid 67 through 79 )BC67 - 7970 - 83
1616chain 'B' and (resid 80 through 89 )BC80 - 8984 - 95
1717chain 'B' and (resid 90 through 107 )BC90 - 10796 - 113
1818chain 'B' and (resid 108 through 119 )BC108 - 119114 - 125
1919chain 'B' and (resid 120 through 131 )BC120 - 131126 - 137
2020chain 'C' and (resid 3 through 25 )CD3 - 251 - 14
2121chain 'C' and (resid 26 through 35 )CD26 - 3515 - 24
2222chain 'C' and (resid 36 through 55 )CD36 - 5525 - 46
2323chain 'C' and (resid 56 through 76 )CD56 - 7647 - 67
2424chain 'C' and (resid 77 through 88 )CD77 - 8868 - 81
2525chain 'C' and (resid 89 through 104 )CD89 - 10482 - 97
2626chain 'D' and (resid 3 through 19 )DE3 - 191 - 8
2727chain 'D' and (resid 20 through 25 )DE20 - 259 - 14
2828chain 'D' and (resid 26 through 35 )DE26 - 3515 - 24
2929chain 'D' and (resid 36 through 54 )DE36 - 5425 - 43
3030chain 'D' and (resid 55 through 88 )DE55 - 8844 - 79
3131chain 'D' and (resid 89 through 103 )DE89 - 10380 - 94

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