+Open data
-Basic information
Entry | Database: PDB / ID: 7mqz | ||||||
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Title | Cytochrome c oxidase assembly factor 7 | ||||||
Components | Cytochrome c oxidase assembly factor 7 | ||||||
Keywords | ELECTRON TRANSPORT / Mitochondria / Complex IV assembly factor | ||||||
Function / homology | Beta-lactamase HcpB-like / Sel1 repeat / Sel1-like repeat / Sel1-like repeats. / mitochondrial intermembrane space / Tetratricopeptide-like helical domain superfamily / mitochondrion / nucleoplasm / Cytochrome c oxidase assembly factor 7 Function and homology information | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.39 Å | ||||||
Authors | Maghool, S. / Maher, M.J. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2022 Title: Mitochondrial COA7 is a heme-binding protein with disulfide reductase activity, which acts in the early stages of complex IV assembly. Authors: Formosa, L.E. / Maghool, S. / Sharpe, A.J. / Reljic, B. / Muellner-Wong, L. / Stroud, D.A. / Ryan, M.T. / Maher, M.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7mqz.cif.gz | 55.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7mqz.ent.gz | 37.8 KB | Display | PDB format |
PDBx/mmJSON format | 7mqz.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/mq/7mqz ftp://data.pdbj.org/pub/pdb/validation_reports/mq/7mqz | HTTPS FTP |
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-Related structure data
Related structure data | 1ouvS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 26155.592 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: COA7, C1orf163, RESA1, SELRC1 / Production host: Escherichia coli K-12 (bacteria) / References: UniProt: Q96BR5 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.69 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.23 M MES sodium, pH 6.72, 2.35 M ammonium sulfate, 8.2% v/v ethanol, 4% v/v pentaerythritol ethoxylate (3/4 EO/OH) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9536 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Sep 12, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9536 Å / Relative weight: 1 |
Reflection | Resolution: 2.39→44.97 Å / Num. obs: 10025 / % possible obs: 99.7 % / Redundancy: 6.8 % / CC1/2: 0.998 / Rmerge(I) obs: 0.093 / Rpim(I) all: 0.039 / Rrim(I) all: 0.101 / Net I/σ(I): 10.5 / Num. measured all: 68093 / Scaling rejects: 4 |
Reflection shell | Resolution: 2.39→2.48 Å / Redundancy: 6.7 % / Rmerge(I) obs: 0.73 / Num. unique obs: 1012 / CC1/2: 0.816 / Rpim(I) all: 0.301 / Rrim(I) all: 0.791 / % possible all: 96.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 1OUV Resolution: 2.39→44.97 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.916 / SU B: 10.294 / SU ML: 0.23 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.364 / ESU R Free: 0.256 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 109.8 Å2 / Biso mean: 56.799 Å2 / Biso min: 31.26 Å2
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Refinement step | Cycle: final / Resolution: 2.39→44.97 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.4→2.448 Å / Rfactor Rfree error: 0
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