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- PDB-7lko: The PilB(N-terminal)-PilZ complex of the Type IV pilus from Xanth... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7lko | ||||||
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Title | The PilB(N-terminal)-PilZ complex of the Type IV pilus from Xanthomonas citri (2.9 A) | ||||||
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![]() | TRANSPORT PROTEIN / Type IV pilus | ||||||
Function / homology | ![]() pilus assembly / cyclic-di-GMP binding / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Llontop, E.E. / Guzzo, C.R. / Farah, C.S. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The PilB-PilZ-FimX regulatory complex of the Type IV pilus from Xanthomonas citri. Authors: Llontop, E.E. / Cenens, W. / Favaro, D.C. / Sgro, G.G. / Salinas, R.K. / Guzzo, C.R. / Farah, C.S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 126.4 KB | Display | ![]() |
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PDB format | ![]() | 84.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18838.270 Da / Num. of mol.: 2 / Fragment: Residues 12-163 Source method: isolated from a genetically manipulated source Details: Expressed with an N-terminal His tag Source: (gene. exp.) ![]() Strain: 306 / Gene: pilB, XAC3239 / Production host: ![]() ![]() #2: Protein | Mass: 12443.409 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: 306 / Gene: pilZ, XAC1133 / Production host: ![]() ![]() #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.44 Å3/Da / Density % sol: 49.52 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop Details: 4 mg/ml protein, 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Jan 6, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54187 Å / Relative weight: 1 |
Reflection | Resolution: 2.88→44.01 Å / Num. obs: 13503 / % possible obs: 99.6 % / Redundancy: 2.1 % / Biso Wilson estimate: 48.15 Å2 / Rmerge(I) obs: 0.06 / Net I/σ(I): 9.6 |
Reflection shell | Resolution: 2.88→2.98 Å / Redundancy: 2 % / Rmerge(I) obs: 0.19 / Num. unique obs: 2614 / % possible all: 97.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PilB-PilZ SeMet model Resolution: 2.88→44.01 Å / SU ML: 0.3117 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 22.4599 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.72 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.88→44.01 Å
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Refine LS restraints |
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LS refinement shell |
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