National Institutes of Health/National Institute of Dental and Craniofacial Research (NIH/NIDCR)
DE028583/DE025567
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI094386
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
1U24GM116792
米国
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
1S10OD018111
米国
National Science Foundation (NSF, United States)
DBI-1338135 and DMR-1548924
米国
引用
ジャーナル: Nat Commun / 年: 2021 タイトル: Structure of human cytomegalovirus virion reveals host tRNA binding to capsid-associated tegument protein pp150. 著者: Yun-Tao Liu / David Strugatsky / Wei Liu / Z Hong Zhou / 要旨: Under the Baltimore nucleic acid-based virus classification scheme, the herpesvirus human cytomegalovirus (HCMV) is a Class I virus, meaning that it contains a double-stranded DNA genome-and no RNA. ...Under the Baltimore nucleic acid-based virus classification scheme, the herpesvirus human cytomegalovirus (HCMV) is a Class I virus, meaning that it contains a double-stranded DNA genome-and no RNA. Here, we report sub-particle cryoEM reconstructions of HCMV virions at 2.9 Å resolution revealing structures resembling non-coding transfer RNAs (tRNAs) associated with the virion's capsid-bound tegument protein, pp150. Through deep sequencing, we show that these RNA sequences match human tRNAs, and we built atomic models using the most abundant tRNA species. Based on our models, tRNA recruitment is mediated by the electrostatic interactions between tRNA phosphate groups and the helix-loop-helix motif of HCMV pp150. The specificity of these interactions may explain the absence of such tRNA densities in murine cytomegalovirus and other human herpesviruses.
履歴
登録
2021年1月28日
登録サイト: RCSB / 処理サイト: RCSB
改定 1.0
2021年11月17日
Provider: repository / タイプ: Initial release
改定 1.1
2024年5月29日
Group: Data collection / カテゴリ: chem_comp_atom / chem_comp_bond
#241 - 2020年1月 20年の分子を振り返って (Twenty Years of Molecules) 類似性 (1)
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集合体
登録構造単位
z: Tegument protein pp150 0: Tegument protein pp150 y: Tegument protein pp150 5: Tegument protein pp150 6: Tegument protein pp150 4: Tegument protein pp150 8: Tegument protein pp150 9: Tegument protein pp150 7: Tegument protein pp150 A: RNA (75-MER) B: RNA (75-MER) C: RNA (75-MER)