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- PDB-7l6t: Crystal Structure of SARS-CoV-2 Nsp16/10 Heterodimer in Complex w... -

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Basic information

Entry
Database: PDB / ID: 7l6t
TitleCrystal Structure of SARS-CoV-2 Nsp16/10 Heterodimer in Complex with (m7GpppA2m)pUpUpApApA (Cap-1), S-Adenosyl-L-homocysteine (SAH) and two Magnesium (Mg) ions.
Components
  • 2'-O-methyltransferase
  • Non-structural protein 10
  • RNA (5'-D(*(M7G))-R(P*(A2M)P*UP*UP*A)-3')
KeywordsVIRAL PROTEIN/RNA / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID / nsp16 / nsp10 / complex / VIRAL PROTEIN / SAH / Cap-1 / VIRAL PROTEIN-RNA complex
Function / homology
Function and homology information


Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Maturation of replicase proteins / Transcription of SARS-CoV-2 sgRNAs / host cell endosome / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Translation of Replicase and Assembly of the Replication Transcription Complex / 5'-3' RNA helicase activity / RNA phosphodiester bond hydrolysis, exonucleolytic / Lyases; Phosphorus-oxygen lyases / modulation by virus of host autophagy ...Assembly of the SARS-CoV-2 Replication-Transcription Complex (RTC) / Maturation of replicase proteins / Transcription of SARS-CoV-2 sgRNAs / host cell endosome / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of TBK1 activity / Translation of Replicase and Assembly of the Replication Transcription Complex / 5'-3' RNA helicase activity / RNA phosphodiester bond hydrolysis, exonucleolytic / Lyases; Phosphorus-oxygen lyases / modulation by virus of host autophagy / mRNA methylation / double membrane vesicle viral factory outer membrane / suppression by virus of host translation / ISG15-specific protease activity / host cell Golgi apparatus / Replication of the SARS-CoV-2 genome / suppression by virus of host type I interferon production / host cell endoplasmic reticulum / SARS coronavirus main proteinase / induction by virus of catabolism of host mRNA / cytoplasmic viral factory / Hydrolases; Acting on ester bonds; Exoribonucleases producing 5'-phosphomonoesters / 3'-5'-exoribonuclease activity / G-quadruplex RNA binding / host cell endoplasmic reticulum-Golgi intermediate compartment / suppression by virus of host ISG15-protein conjugation / protein K48-linked deubiquitination / suppression by virus of host toll-like receptor signaling pathway / suppression by virus of host viral-induced cytoplasmic pattern recognition receptor signaling pathway via inhibition of IRF3 activity / transcription, RNA-templated / suppression by virus of host NF-kappaB cascade / viral transcription / modulation by virus of host protein ubiquitination / protein K63-linked deubiquitination / methyltransferase cap1 / positive stranded viral RNA replication / protein autoprocessing / cysteine-type peptidase activity / viral genome replication / mRNA (nucleoside-2'-O-)-methyltransferase activity / suppression by virus of host TRAF activity / helicase activity / ubiquitinyl hydrolase 1 / DNA helicase / thiol-dependent deubiquitinase / DNA helicase activity / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / single-stranded RNA binding / host cell perinuclear region of cytoplasm / viral protein processing / RNA helicase / induction by virus of host autophagy / endonuclease activity / RNA-directed RNA polymerase / cysteine-type endopeptidase activity / viral RNA genome replication / RNA-directed 5'-3' RNA polymerase activity / suppression by virus of host type I interferon-mediated signaling pathway / transcription, DNA-templated / host cell cytoplasm / protein dimerization activity / : / protein homodimerization activity / zinc ion binding / integral component of membrane / ATP binding / identical protein binding / cytosol
Similarity search - Function
RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 14, betacoronavirus / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. ...RNA-dependent RNA polymerase, SARS-CoV-like / Nonstructural protein 15, middle domain, alpha/betacoronavirus / Nonstructural protein 14, betacoronavirus / Coronavirus (CoV) Nsp15 N-terminal oligomerization domain profile. / Nidovirus 3'-5' exoribonuclease (ExoN) domain profile. / Coronavirus Nsp12 RNA-dependent RNA polymerase (RdRp) domain profile. / Coronavirus (CoV) guanine-N7-methyltransferase (N7-MTase) domain profile. / Nidovirus 2'-O-methyltransferase (2'-O-MTase) domain profile. / Nidovirus RdRp-associated nucleotidyl transferase (NiRAN) domain profile. / Arterivirus Nsp11 N-terminal/coronavirus NSP15 middle (AV-Nsp11N/CoV-Nsp15M) domain profile. / Nidoviral uridylate-specific endoribonuclease (NendoU) domain profile. / Viral (Superfamily 1) RNA helicase / Nonstructural protein 15, N-terminal domain, alpha/beta-coronavirus / NSP15, NendoU domain, coronavirus / Nonstructural protein 13, 1B domain, coronavirus / Coronavirus 2'-O-methyltransferase / Coronavirus RNA-dependent RNA polymerase, N-terminal / Coronavirus replicase NSP15, middle domain / Coronavirus replicase NSP15, N-terminal oligomerisation / Non-structural protein NSP16, coronavirus-like / Non-structural protein NSP15, middle domain superfamily / Non-structural protein 14, coronavirus / Non-structural protein NSP15, N-terminal domain superfamily, coronavirus / Coronavirus proofreading exoribonuclease / Coronavirus replicase NSP15, middle domain / RNA polymerase, N-terminal, coronavirus / Coronavirus replicase NSP15, N-terminal oligomerisation / Nonstructural protein 13, zinc-binding domain, coronavirus-like / Coronaviridae zinc-binding (CV ZBD) domain profile. / Sarbecovirus Nsp3c-N domain profile. / Polyprotein cleavage domain PL2pro superfamily, coronavirus / Non-structural protein NSP3, N-terminal, betacoronavirus / Non-structural protein NSP1 superfamily, betacoronavirus / Non-structural protein NSP3, SUD-N (Mac2) domain superfamily, betacoronavirus / Betacoronavirus replicase NSP3, N-terminal / Betacoronavirus SUD-C domain / Non-structural protein 2, SARS-CoV-like / Coronavirus replicase NSP15, uridylate-specific endoribonuclease / NendoU domain, nidovirus / Endoribonuclease EndoU-like / Carbamoyl-phosphate synthase subdomain signature 2. / Betacoronavirus (BetaCoV) Nsp1 C-terminal domain profile. / (+)RNA virus helicase core domain profile. / (+) RNA virus helicase core domain / Betacoronavirus Nsp3c-M domain profile. / Betacoronavirus single-stranded poly(A) binding domain / Non-structural protein NSP1, betacoronavirus / Betacoronavirus replicase NSP1 / Non-structural protein NSP3, SUD-M domain superfamily, betacoronavirus / Non-structural protein NSP3, SUD-M domain, betacoronavirus / Coronavirus (CoV) Nsp1 globular domain profile. / Betacoronavirus Nsp3e nucleic acid-binding (NAB) domain profile. / Betacoronavirus Nsp3c-C domain profile. / DPUP/SUD, C-terminal, betacoronavirus / Non-structural protein 6, betacoronavirus / Replicase polyprotein, nucleic acid-binding domain superfamily / Betacoronavirus nucleic acid-binding (NAB) / Non-structural protein NSP3, nucleic acid-binding domain, betacoronavirus / Non-structural protein NSP3A domain-like superfamily / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp7 cofactor domain profile. / Coronavirus RNA-dependent RNA polymerase (RdRp) Nsp8 cofactor domain profile. / Coronavirus Nsp9 single-stranded RNA (ssRNA)-binding domain profile. / Coronavirus (CoV) ExoN/MTase coactivator domain profile. / Papain-like protease, N-terminal domain superfamily, coronavirus / Lipocalin signature. / Papain-like viral protease, palm and finger domains, coronavirus / Coronavirus replicase NSP2, N-terminal / Nonstructural protein 2, N-terminal domain, coronavirus / Coronavirus Nsp3a Ubl domain profile. / Coronavirus Nsp3d Ubl domain profile. / Non-structural protein 2, C-terminal domain, coronavirus / Coronavirus replicase NSP2, C-terminal / NSP3, first ubiquitin-like (Ubl) domain, coronavirus / Coronavirus Nsp4 C-terminal (Nsp4C) domain profile. / NSP3, second ubiquitin-like (Ubl) domain, coronavirus / Papain-like protease, thumb domain superfamily, coronavirus / Non-structural protein NSP7, coronavirus / Coronavirus replicase NSP7 / Peptidase family C16 domain profile. / Coronavirus replicase NSP8 / Coronavirus papain-like peptidase / Coronavirus replicase NSP4, N-terminal / Coronavirus replicase NSP4, C-terminal / Coronavirus replicase NSP3, C-terminal / Non-structural protein 6, coronavirus / Coronavirus RNA synthesis protein NSP10 / Non-structural protein NSP9 superfamily, coronavirus / Coronavirus replicase NSP6 / RNA synthesis protein NSP10 superfamily, coronavirus / Peptidase C30, coronavirus / Peptidase C16, coronavirus / Non-structural protein NSP9, coronavirus / Non-structural protein NSP8, coronavirus-like / RNA synthesis protein NSP10, coronavirus / Non-structural protein NSP4, C-terminal, coronavirus / Coronavirus endopeptidase C30 / Coronavirus replicase NSP4, N-terminal / Non-structural protein NSP7 superfamily, coronavirus / Coronavirus main protease (M-pro) domain profile. / Non-structural protein NSP8 superfamily, coronavirus
Similarity search - Domain/homology
beta-D-fructopyranose / RNA / FORMIC ACID / S-ADENOSYL-L-HOMOCYSTEINE / alpha-D-glucopyranose / Replicase polyprotein 1ab
Similarity search - Component
Biological speciesSevere acute respiratory syndrome coronavirus 2
synthetic construct (others)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.78 Å
AuthorsMinasov, G. / Shuvalova, L. / Rosas-Lemus, M. / Kiryukhina, O. / Brunzelle, J.S. / Satchell, K.J.F. / Center for Structural Genomics of Infectious Diseases (CSGID)
CitationJournal: Sci.Signal. / Year: 2021
Title: Mn 2+ coordinates Cap-0-RNA to align substrates for efficient 2'- O -methyl transfer by SARS-CoV-2 nsp16.
Authors: Minasov, G. / Rosas-Lemus, M. / Shuvalova, L. / Inniss, N.L. / Brunzelle, J.S. / Daczkowski, C.M. / Hoover, P. / Mesecar, A.D. / Satchell, K.J.F.
History
DepositionDec 23, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 6, 2021Provider: repository / Type: Initial release
Revision 1.1Dec 15, 2021Group: Database references / Category: citation / citation_author / database_2
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 2'-O-methyltransferase
B: Non-structural protein 10
C: RNA (5'-D(*(M7G))-R(P*(A2M)P*UP*UP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)52,08613
Polymers50,9003
Non-polymers1,18610
Water9,152508
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: homology
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
γ
α
β
Length a, b, c (Å)169.351, 169.351, 52.629
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number152
Space group name H-MP3121

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Components

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Protein , 2 types, 2 molecules AB

#1: Protein 2'-O-methyltransferase / NSP16


Mass: 33556.426 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Plasmid: pMCSG53 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): magic
References: UniProt: P0DTD1, Transferases; Transferring one-carbon groups; Methyltransferases
#2: Protein Non-structural protein 10 / NSP10


Mass: 15004.114 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: rep, 1a-1b / Plasmid: pMCSG53 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / Variant (production host): magic / References: UniProt: P0DTD1

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RNA chain , 1 types, 1 molecules C

#3: RNA chain RNA (5'-D(*(M7G))-R(P*(A2M)P*UP*UP*A)-3')


Mass: 2339.452 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)

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Sugars , 2 types, 3 molecules

#8: Sugar ChemComp-GLC / alpha-D-glucopyranose / alpha-D-glucose / D-glucose / glucose / Glucose


Type: D-saccharide, alpha linking / Mass: 180.156 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H12O6
IdentifierTypeProgram
DGlcpaCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
a-D-glucopyranoseCOMMON NAMEGMML 1.0
a-D-GlcpIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcSNFG CARBOHYDRATE SYMBOLGMML 1.0
#10: Sugar ChemComp-BDF / beta-D-fructopyranose / beta-D-fructose / D-fructose / fructose / Fructose


Type: D-saccharide, beta linking / Mass: 180.156 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H12O6
IdentifierTypeProgram
DFrupbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
b-D-fructopyranoseCOMMON NAMEGMML 1.0
b-D-FrupIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
FruSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 6 types, 515 molecules

#4: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Cl
#6: Chemical ChemComp-FMT / FORMIC ACID / Formic acid


Mass: 46.025 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: CH2O2
#7: Chemical ChemComp-SAH / S-ADENOSYL-L-HOMOCYSTEINE / S-Adenosyl-L-homocysteine


Type: L-peptide linking / Mass: 384.411 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C14H20N6O5S / Feature type: SUBJECT OF INVESTIGATION
#9: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn / Feature type: SUBJECT OF INVESTIGATION
#11: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 508 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.39 Å3/Da / Density % sol: 72 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop / pH: 7.5
Details: Protein: 3.8 mg/ml (nsp10/nsp16 1:1), 0.15M Sodium chloride, 0.01M Tris pH 7.5 , 2mM SAM, 1mM TCEP, 5% Glycerol;Screen: Classics II (B3), 0.5M Magnesium formate, 0.1M HEPES pH 7.5;Soak: ...Details: Protein: 3.8 mg/ml (nsp10/nsp16 1:1), 0.15M Sodium chloride, 0.01M Tris pH 7.5 , 2mM SAM, 1mM TCEP, 5% Glycerol;Screen: Classics II (B3), 0.5M Magnesium formate, 0.1M HEPES pH 7.5;Soak: 6hours, 0.2mM m7GpppAUUAAA, 5mM SAM, in screen solution;Cryo: 25% Sucrose in screen solution.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 1.12708 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Oct 5, 2020 / Details: Be
RadiationMonochromator: Diamond / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.12708 Å / Relative weight: 1
ReflectionResolution: 1.78→30 Å / Num. obs: 81048 / % possible obs: 96.6 % / Observed criterion σ(I): -3 / Redundancy: 6.8 % / Biso Wilson estimate: 24.4 Å2 / CC1/2: 0.996 / CC star: 0.999 / Rmerge(I) obs: 0.09 / Rpim(I) all: 0.036 / Rrim(I) all: 0.097 / Rsym value: 0.09 / Χ2: 1.01 / Net I/σ(I): 20.8
Reflection shellResolution: 1.78→1.81 Å / Redundancy: 5.8 % / Rmerge(I) obs: 1.156 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 3493 / CC1/2: 0.56 / CC star: 0.848 / Rpim(I) all: 0.513 / Rrim(I) all: 1.269 / Rsym value: 1.156 / Χ2: 1.002 / % possible all: 84.2

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Processing

Software
NameVersionClassification
REFMAC5.8.0267refinement
PDB_EXTRACT3.25data extraction
HKL-3000data reduction
HKL-3000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6w4h
Resolution: 1.78→29.33 Å / Cor.coef. Fo:Fc: 0.975 / Cor.coef. Fo:Fc free: 0.968 / SU B: 2.926 / SU ML: 0.046 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.067 / ESU R Free: 0.068 / Stereochemistry target values: MAXIMUM LIKELIHOOD
Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
RfactorNum. reflection% reflectionSelection details
Rfree0.1615 3950 4.9 %RANDOM
Rwork0.1408 ---
obs0.1418 76229 96.81 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso max: 137.25 Å2 / Biso mean: 33.036 Å2 / Biso min: 15.74 Å2
Baniso -1Baniso -2Baniso -3
1-0.75 Å20.38 Å20 Å2
2--0.75 Å20 Å2
3----2.43 Å2
Refinement stepCycle: final / Resolution: 1.78→29.33 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms3191 96 70 531 3888
Biso mean--35.61 45.52 -
Num. residues----418
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0060.0133630
X-RAY DIFFRACTIONr_bond_other_d0.0010.0173272
X-RAY DIFFRACTIONr_angle_refined_deg1.331.664974
X-RAY DIFFRACTIONr_angle_other_deg0.3791.6077575
X-RAY DIFFRACTIONr_dihedral_angle_1_deg3.4215449
X-RAY DIFFRACTIONr_dihedral_angle_2_deg29.23923.812160
X-RAY DIFFRACTIONr_dihedral_angle_3_deg8.89815578
X-RAY DIFFRACTIONr_dihedral_angle_4_deg7.691512
X-RAY DIFFRACTIONr_chiral_restr0.0640.2488
X-RAY DIFFRACTIONr_gen_planes_refined0.0540.024138
X-RAY DIFFRACTIONr_gen_planes_other0.0480.02825
LS refinement shellResolution: 1.78→1.826 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.256 262 -
Rwork0.239 5247 -
all-5509 -
obs--90.83 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
14.0583-0.1311.42561.04960.13552.85150.009-0.12260.45110.10830.0116-0.1265-0.05420.1363-0.02060.0956-0.0034-0.00950.0199-0.0420.169294.87438.58632.01
20.9911-0.39620.03981.28830.16521.17180.03930.0057-0.0258-0.03870.0365-0.0070.21650.0272-0.07580.15520.0133-0.02030.00290.00280.093890.19921.76123.303
31.4617-0.18540.48611.09680.30731.52820.0302-0.0290.19310.01770.0479-0.1010.01410.1039-0.07810.0887-0.00010.00040.0123-0.00190.139492.75333.80526.968
413.9082-8.5-2.271514.29511.15428.3250.31550.90780.5197-0.5802-0.2046-0.79190.33920.3928-0.11090.19290.0320.08180.12050.00620.0668102.65722.8685.094
56.38612.9354-0.64789.71342.16546.63020.13790.0817-0.1664-0.40150.0818-1.04620.0540.6181-0.21970.15550.07140.09860.12770.00180.1415107.34622.7917.82
60.26781.7604-1.550512.9375-13.624718.4668-0.00290.02850.05610.21710.31770.3454-0.1214-0.5385-0.31470.25980.00210.01350.01780.00510.100691.012-0.96121.214
74.53874.1156-1.886963.7436-6.35541.20240.4105-0.7546-0.4237-1.5379-0.8161-0.94130.00080.37020.40570.1415-0.040.04130.19350.20360.645961.193-2.13513.283
813.9745-2.5522-3.657718.82298.82058.03610.0109-0.3563-0.7609-0.1063-0.27561.34470.4779-0.97270.26470.112-0.1364-0.12820.28360.03980.502755.0248.93316.568
92.8173-0.05470.54973.005-0.61971.89410.10620.1796-0.0526-0.1927-0.06420.38110.0558-0.2467-0.0420.1289-0.0134-0.08780.0972-0.00940.198268.66924.16213.442
104.2651-1.04990.26834.0846-0.6483.55350.07610.4587-0.0233-0.4356-0.04710.37670.14-0.3237-0.02890.1557-0.0058-0.13890.1047-0.00740.130270.41824.337.761
115.58720.2802-0.67548.9415-3.41332.655-0.06980.4963-0.8464-1.05020.00370.40970.7495-0.15320.06610.4088-0.0593-0.14340.0891-0.11250.326371.2387.2249.782
123.2081-0.2338-1.24833.482-1.23993.6950.05830.3713-0.4151-0.52430.02930.72180.3077-0.6211-0.08760.2134-0.0647-0.20740.2053-0.05480.29563.1719.177.866
1317.2619-4.6665-2.91028.3277-5.182318.57250.24851.1877-0.3243-0.6939-0.08420.87970.1912-1.334-0.16430.388-0.0438-0.31860.4844-0.04760.292460.35222.36-3.033
140.44870.32-0.44369.0786-0.90562.7049-0.0071-0.11640.07510.4268-0.0021-0.01730.143-0.12930.00930.20720.013-0.00520.08190.0030.086785.44727.16341.086
1510.0795-8.3932-1.21998.538-0.64441.9355-0.14640.1327-0.5246-0.1220.07080.45240.3154-0.21780.07560.2408-0.04780.01190.1062-0.0180.201882.73521.39633.143
166.77444.48430.155418.57010.69770.05070.0178-0.1635-0.55130.62330.0101-0.37510.038-0.0415-0.02790.1683-0.0355-0.01840.08010.04770.148778.6620.72430.513
178.8954-7.96452.84818.28511.33442.26590.0174-0.19260.00010.3788-0.07250.17560.1468-0.15860.05510.1301-0.0340.01050.10280.04250.131374.31126.5230.864
186.57890.05411.14127.6065-0.19453.5013-0.0653-0.2567-0.10350.14740.28280.30710.2784-0.258-0.21750.2041-0.0095-0.00960.03790.00550.137284.68415.36227.64
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A6798 - 6822
2X-RAY DIFFRACTION2A6823 - 7011
3X-RAY DIFFRACTION3A7012 - 7050
4X-RAY DIFFRACTION4A7051 - 7062
5X-RAY DIFFRACTION5A7063 - 7085
6X-RAY DIFFRACTION6A7086 - 7096
7X-RAY DIFFRACTION7B4272 - 4277
8X-RAY DIFFRACTION8B4278 - 4287
9X-RAY DIFFRACTION9B4288 - 4307
10X-RAY DIFFRACTION10B4308 - 4326
11X-RAY DIFFRACTION11B4327 - 4348
12X-RAY DIFFRACTION12B4349 - 4372
13X-RAY DIFFRACTION13B4373 - 4385
14X-RAY DIFFRACTION14C0
15X-RAY DIFFRACTION15C1
16X-RAY DIFFRACTION16C2
17X-RAY DIFFRACTION17C3 - 4
18X-RAY DIFFRACTION18A7104

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