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- PDB-7krg: Crystal Structure of Mannitol Dehydrogenase (ChMDH) from Cladospo... -

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Basic information

Entry
Database: PDB / ID: 7krg
TitleCrystal Structure of Mannitol Dehydrogenase (ChMDH) from Cladosporium herbarum in complex with NADP+ and Na
ComponentsNADP-dependent mannitol dehydrogenase
KeywordsOXIDOREDUCTASE / Mannitol Dehydrogenase / ChMDH
Function / homology
Function and homology information


mannitol 2-dehydrogenase (NADP+) / mannitol 2-dehydrogenase (NADP+) activity / mannitol metabolic process / oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor / NADP binding / protein homotetramerization / protein homodimerization activity
Similarity search - Function
Enoyl-(Acyl carrier protein) reductase / Short-chain dehydrogenase/reductase SDR / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADP-dependent mannitol dehydrogenase
Similarity search - Component
Biological speciesCladosporium herbarum (fungus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.03797368098 Å
AuthorsLee, S.G. / Jez, J.M.
Funding support United States, 1items
OrganizationGrant numberCountry
National Science Foundation (NSF, United States) United States
CitationJournal: Transgenic Res / Year: 2022
Title: Biochemical and clinical studies of putative allergens to assess what distinguishes them from other non-allergenic proteins in the same family.
Authors: Glenn, K.C. / Silvanovich, A. / Lee, S.G. / Allen, A. / Park, S. / Dunn, S.E. / Kessenich, C. / Meng, C. / Vicini, J.L. / Jez, J.M.
History
DepositionNov 19, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 23, 2022Provider: repository / Type: Initial release
Revision 1.1Sep 6, 2023Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 18, 2023Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: NADP-dependent mannitol dehydrogenase
B: NADP-dependent mannitol dehydrogenase
C: NADP-dependent mannitol dehydrogenase
D: NADP-dependent mannitol dehydrogenase
E: NADP-dependent mannitol dehydrogenase
F: NADP-dependent mannitol dehydrogenase
G: NADP-dependent mannitol dehydrogenase
H: NADP-dependent mannitol dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)236,27420
Polymers230,2358
Non-polymers6,03912
Water25,4731414
1
A: NADP-dependent mannitol dehydrogenase
B: NADP-dependent mannitol dehydrogenase
C: NADP-dependent mannitol dehydrogenase
D: NADP-dependent mannitol dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)118,13710
Polymers115,1174
Non-polymers3,0206
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area21900 Å2
ΔGint-127 kcal/mol
Surface area32120 Å2
MethodPISA
2
F: NADP-dependent mannitol dehydrogenase
H: NADP-dependent mannitol dehydrogenase
hetero molecules

E: NADP-dependent mannitol dehydrogenase
hetero molecules

G: NADP-dependent mannitol dehydrogenase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)118,13710
Polymers115,1174
Non-polymers3,0206
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation1_554x,y,z-11
crystal symmetry operation2_845-x+3,y-1/2,-z1
Buried area21800 Å2
ΔGint-127 kcal/mol
Surface area32340 Å2
MethodPISA
Unit cell
Length a, b, c (Å)88.867, 119.322, 111.211
Angle α, β, γ (deg.)90.000, 94.884, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

#1: Protein
NADP-dependent mannitol dehydrogenase / MtDH / Mannitol 2-dehydrogenase [NADP(+)]


Mass: 28779.330 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Cladosporium herbarum (fungus) / Production host: Escherichia coli (E. coli)
References: UniProt: P0C0Y5, mannitol 2-dehydrogenase (NADP+)
#2: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Na / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-NAP / NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / 2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE


Mass: 743.405 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C21H28N7O17P3 / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1414 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.58 Å3/Da / Density % sol: 52.32 %
Crystal growTemperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 25% PEG 1500, 100 mM sodium propionate/sodium cacodylate/BIS-TRIS propane (2:1:2 molar ratio), pH 7, 5 mM NADP+

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.979 Å
DetectorType: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 10, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.0379→50 Å / Num. obs: 141106 / % possible obs: 97.6 % / Redundancy: 2.9 % / Biso Wilson estimate: 27.8196736865 Å2 / Rrim(I) all: 0.137 / Rsym value: 0.087 / Net I/σ(I): 16.6
Reflection shellResolution: 2.0379→2.09 Å / Num. unique obs: 7165 / CC1/2: 0.656

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Processing

Software
NameVersionClassification
PHENIX1.11.1_2575refinement
PHENIX1.11.1_2575refinement
SBC-Collect1.14_3260data collection
Cootmodel building
HKL-3000data scaling
PHASERphasing
HKL-3000data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3GDF

3gdf


Resolution: 2.03797368098→31.4045238396 Å / SU ML: 0.223630714574 / Cross valid method: FREE R-VALUE / σ(F): 1.3435622572 / Phase error: 20.3699230828
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.182916664342 1990 1.41089723138 %
Rwork0.154135383197 139055 -
obs0.154547684041 141045 95.8485668076 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 32.9031283964 Å2
Refinement stepCycle: LAST / Resolution: 2.03797368098→31.4045238396 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15923 0 388 1414 17725
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0070366445929316633
X-RAY DIFFRACTIONf_angle_d0.82881789726922561
X-RAY DIFFRACTIONf_chiral_restr0.05084013528472488
X-RAY DIFFRACTIONf_plane_restr0.00416078031752882
X-RAY DIFFRACTIONf_dihedral_angle_d4.930404450679613
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.038-2.08890.278526894939910.2372211671327719X-RAY DIFFRACTION74.9304422911
2.0889-2.14540.3030620688021610.21324358236110215X-RAY DIFFRACTION98.9509822621
2.1454-2.20850.2562697571611500.20347004110610243X-RAY DIFFRACTION99.1320106829
2.2085-2.27980.2573000645191440.19385480302510197X-RAY DIFFRACTION98.6548368632
2.2798-2.36120.2205541729831540.17960771197610187X-RAY DIFFRACTION98.6924985684
2.3612-2.45570.2064525045051230.17701335633710188X-RAY DIFFRACTION98.6415383144
2.4557-2.56750.2327933197081620.16228876505210167X-RAY DIFFRACTION98.3152484295
2.5675-2.70270.1842973172851430.15838095068110164X-RAY DIFFRACTION98.0591761012
2.7027-2.8720.1921186932831470.1505975737910116X-RAY DIFFRACTION97.8546910755
2.872-3.09350.1936241440651440.15121606814310095X-RAY DIFFRACTION97.3196464214
3.0935-3.40450.1670513452331450.14493755614710068X-RAY DIFFRACTION96.9711355868
3.4045-3.89640.171462112391460.1320272054159953X-RAY DIFFRACTION95.9433783013
3.8964-4.9060.1349575660951340.1219427912859914X-RAY DIFFRACTION95.0614947966
4.906-31.40.1377198637441460.1509672601859829X-RAY DIFFRACTION93.276603703
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.383747225493-0.1983216347940.4038184906120.5615722626990.1452354385990.5751342106470.08920750494640.0288178540729-0.103474220241-0.00372190245137-0.0235324039261-0.08133889384510.06842019272480.105413237646.91725472625E-50.2030354498390.03762673754630.0228337628460.2017443859830.003738287465210.213634573141185.160539278-31.895289247632.6484196064
20.194083876327-0.3052189729320.02812922046360.4655644411170.170728927160.4074832459860.0003860707560480.01502413783930.002786462534140.0929578514066-0.0113882983887-0.04608477411960.01588969992660.007616493312952.4484140884E-50.1793881555660.0116470685690.01766812677190.1895400615160.02409917909410.221731252583180.731832534-17.271459649240.6893081493
30.388500768099-0.2680257068180.2670377274560.5045076956130.2341794974720.400967670021-0.0170965985348-0.0803398604946-0.05978617358590.06664260581470.008030322202360.07200785520140.06557250711990.0278824416159-3.87980791256E-60.2006798474220.02142757596270.0282050999360.1962441195450.0105309204020.190602387829169.740246457-23.236382871132.4166154614
40.311670726649-0.179700065780.185151521220.5969687351310.2682815459330.726083091139-0.01358688852-0.09856062021790.0519594303060.0559158105177-0.0115745995195-0.0235848259056-0.106914386819-0.00100793333759.26110080121E-50.21982972469-0.004922119289960.01030933388340.206174439996-0.01906493452170.203298802629165.38728747312.889514909352.9055951147
50.0751607055103-0.0939499885566-0.121207861290.5912742914080.2012635784420.3188975184920.0224274014364-0.0001652017063310.09147680942480.003025292139380.00265118931165-0.0696452444264-0.04133189301120.006711677911570.0001217450849970.157619980285-0.004306926264350.01932076273790.1793063336410.009308291186990.164696843141173.150565705-1.6864389877145.3281260282
60.270650873827-0.290925391685-0.03638013024050.3349509742850.2716194207670.4382417048820.0466369080581-0.1374665850880.08230475695660.0401239363124-0.0364768243418-0.167974200174-0.1419153126420.1447586081120.001593080012420.180962053275-0.01984448232410.01858794305390.1941406290650.001582259318140.218372374807174.9933149727.9439991873135.1460643497
70.0600692655219-0.008157441903830.1231895900390.227942799289-0.2117179931190.142354849544-0.0361349472521-0.008908154221420.0111328568871-0.165675388020.01262878005210.0795367216592-0.06543265384240.02204818092323.7143300686E-50.2088376609780.00524191351388-0.001059788015730.194566452399-0.01684609054130.203241523692163.2497231796.9595631776136.2266118739
80.493631459457-0.352220263790.3066336439530.3774857274670.1446888846460.789405899748-0.116886816937-0.09402755454610.0408164203704-0.1229641989570.007537613810930.141547404848-0.303019130199-0.153777183246-0.002553035583030.2702945029970.0732020750542-0.03674571950580.214847575724-0.04119525509770.279114437664146.54139116516.227716241326.2711072906
90.20008494633-0.2042732723790.1765279624370.136775068298-0.05023592289620.204597194635-0.0536659168382-0.00922475930619-0.0266218438113-0.06945558148370.007660164228440.141052435327-0.107678733233-0.08016972455771.68622661502E-50.2680788424040.0366632726919-0.02919944048840.206301650998-0.02324574667380.263210032514148.1436566187.7013926810815.0402261035
100.1108643358150.003311639651960.008598101643680.0240189625258-0.05872871774420.113432385719-0.001186118741090.1365073783770.0849531087525-0.303870286376-0.0500587859970.371456329996-0.106592786319-0.126985971212-0.0002699818121160.2656017323560.0401012602968-0.02804083214370.206652527207-0.01217875468640.246039580255153.1955972384.3667642986612.3586150382
110.123553169534-0.07702219716860.03566670663420.09128335830050.08022159410620.1827870036970.0242206752679-0.0770455576417-0.049476606013-0.0813533466773-0.09406081209340.0441448447828-0.00295564136099-0.0322257371277-2.7101925513E-50.2227515082610.0264836439656-0.01306466001490.2023240630310.003309693518230.239723378792156.183196875-2.6587488634322.5480925002
120.164263323145-0.278095553781-0.00656767799650.3176683828590.1272815305370.326341547802-0.004030214944580.005536647240940.0533841674893-0.165095694261-0.04306261544350.3086547161570.0512826547035-0.09064210302914.85090261727E-50.210682124148-0.00168357590993-0.008257576543310.213480358769-0.007927672261160.272107971196148.629532937-3.0350658725531.088953605
130.1029985989930.07271824022490.100354079034-0.01042620013830.07451797635250.1542773398280.01130001933810.0102385765856-0.00282958023359-0.0294929188041-0.04251536412160.0581429928719-0.0693500945871-0.0611683572446-1.04132096971E-50.2085958104540.0201296074571-0.002608184442330.206980176823-0.01328899212860.205722288827156.8743394325.1864232094133.1246964379
140.464868966542-0.1538217094770.2589863610040.254673832710.1511178853380.9435945652080.1063302558460.114551243186-0.112046339666-0.17913555512-0.03746002618530.04745962793710.1283211580280.08686015338460.0002404024063420.2859781536990.0588806455692-0.00939545504390.191906973495-0.03181671672780.21590550448167.737230053-27.74425090685.26248758287
150.325564224698-0.1811699913820.3022439258130.3751102343950.1390727308420.3405523192640.06506599154220.0612559566912-0.0263849221307-0.192847965175-0.05992088293570.0452187722462-0.00381305531310.0588863770132.99625508133E-50.2304010061530.0315457230050.00273237254470.187824380398-0.003822180928810.183447646498161.562595188-11.864654128811.6821491181
160.325694408493-0.1408186914390.2198894110140.4122371824760.2386529769850.276929871540.008520687962190.147082736042-0.021874836404-0.32916111625-0.0174708957241-0.03964999842910.01290024396090.09588424771850.003470291766810.246046465260.02067544445680.03586557615340.2238743890440.02990819825140.186870792508175.488429853-10.013799416212.9313371256
170.119356047545-0.127009835237-0.04695396102290.0375550863107-0.008179526255240.09127085968130.0895536300092-0.02999965966640.0117253084079-0.123197320659-0.01284803145550.141537780360.0439767372190.0255791490117-3.00701761275E-50.2320073108150.01944066916810.02086229625330.1803415093060.003882871776180.193084365844173.16973036-19.240423817719.8840984151
180.9277763833570.5366602118240.3773061816890.443136144611-0.02784062826530.606675742399-0.2247252089680.401204913510.190715268099-0.08958925062370.1408632389370.101164257509-0.0477179943704-0.00769049425744-0.05578901109620.242980945291-0.119238150121-0.05863249085530.2855526962670.1309006552630.183495205749124.420459676.5733694160962.2848894664
190.4303143422620.137819670462-0.07562978347950.125258613565-0.2007028431280.435854633108-0.0136367720310.2555232534970.208372783835-0.18253919550.02252671700820.0872053760009-0.169539962835-0.07972124828-0.0001379404175280.296641232216-0.00963672964992-0.05794204685570.2513167358340.06371886634180.357682918777122.33509800714.46442522275.4074937283
200.2587518852980.2507622938630.1679906101720.238745227390.04941735262220.0679475322507-0.08504268934490.2324759888440.07101978730310.01297979403210.02150116512590.0653084383213-0.0419648816504-0.0309069420949-0.0001633585841540.265857347916-0.067718759375-0.02141251518410.2426631062460.06236658394840.243353064243130.9816072446.3417171151575.0175204145
211.071285952750.2817084753-0.0218615600452-0.0546522010369-0.02545691136910.5296264165160.0239624315856-0.22895425635-0.02832375230250.03723045512980.0008764686360370.0236490786536-0.0018743025881-0.05967269934153.98552478277E-50.216866157105-0.005478624388490.005529482500410.2595084189060.0419137031590.221826105156125.675082786-8.84936288977-3.33157046956
220.5504767248510.0396897927463-0.1143710466460.108966078589-0.04834437779710.295525559285-0.0313322320127-0.1331435650480.09914484670760.07716300146790.0382250595713-0.0274644703696-0.00731445233421-0.07031609403491.28307967887E-50.1929616581340.00124508541665-0.02508771492640.2067088197560.01217036898790.267005449316120.8413817670.87586959534-12.126968956
23-0.00324029704463-0.02159591712370.006940494844820.020613085706-0.01465639732140.0137479655034-0.1283431558550.3836214065740.1551513110870.12141629299-0.0104598663398-0.444416882589-0.3270510765950.3194858699363.27414145523E-50.337735023677-0.02972463679840.001324277671420.3666694249690.01820395860390.399914359697161.55169535159.83104371039.16769105198
240.156789583947-0.125980067863-0.01486943342980.0630316671009-0.03643217782620.0135468774810.02504051127070.163440175237-0.2206346145040.01643194818210.0029667531624-0.05659230962080.1159798035350.140075245392-1.72139087104E-50.2246364883040.0580097664834-0.0161591829060.291320816665-0.04216447102970.321550736984165.45520785248.035932515426.6245125804
250.0141778819272-0.01886207870310.00834432475350.07139659474910.007367563674190.01110021479630.169992044255-0.168733109474-0.6442964716580.2217130341960.171633584245-0.2565862903180.2570455050730.1303038333850.01466823887730.3251376956930.0773419575507-0.03740823932180.386875104887-0.05727752338980.488516394232172.25707014541.23990602428.6099369418
260.0142134796450.0255870056907-0.008501936734990.01197004869120.002465297045070.00738209506369-0.0166552403269-0.1020530572250.003559274246670.025529567594-0.00125982925229-0.120651461298-0.09045653868720.1695178860233.23538544561E-50.2965850191520.0932487825549-0.05263711449340.42103210545-0.04628317857530.365288488682169.34009583452.019563774935.0288968339
270.126210168439-0.04494376361870.004522685127890.01079285209480.06675133917410.147204729429-0.138071189465-0.159569583388-0.04344997777380.04859863357320.0595769423018-0.162054318745-0.0007299754980540.0818096260017-1.97176314672E-50.2215036716710.029607307806-0.05102655232270.263008651426-0.02014984160540.289997937799161.81376338855.869613499637.0571246349
280.4104690349740.00398793091103-0.2874962062030.135225777798-0.3163090790450.562971432389-0.269101785293-0.4009919155070.175387185862-0.06779576160060.00546893660759-0.1193980186210.1677976840010.294000390313-0.01228748961130.2562697214280.0333661559464-0.07806439122550.322734371736-0.05638043996130.345642994028157.42118490360.603096984638.4397633587
290.184860151236-0.2102666956770.09949052415930.222066848681-0.0900618594798-0.00378194706011-0.0638127268684-0.042657550768-0.0506439785030.03614255769510.0334450569356-0.0550753774408-0.004330098311060.04210561469485.48097126198E-60.249358374520.0317207960257-0.03230097341080.25500542707-0.03569330251950.27378639888147.25056590955.226400383932.9927758258
300.361106924724-0.154789154509-0.086727349020.0522504979556-0.01593384312580.0929047580914-0.0419265840119-0.133574883352-0.08959138904650.15496510184-0.00149665782605-0.142012650931-0.004100853289350.138926485960.0008514185692350.2414291039050.031403390803-0.04651964712480.2325358627970.01347874387910.294352236498152.38906662451.434379130729.8518472976
310.107045264102-0.04425205356490.06879220365030.06441935479720.05800549291740.126985611409-0.0522931938284-0.0136904666148-0.1503505429830.2302152384460.0953333597934-0.02798505183690.09892227923880.09160634374742.73632647188E-50.276765049020.05237028593-0.003176383012150.23448843567-0.01941885910620.317151058647147.7383303940.256886555426.6009761363
320.101191242705-0.1414934568120.007135616581820.2002553854180.1053860289740.1097600974490.007200457279680.02817532359790.05655923306720.1324871953850.02315203850980.0168712246384-0.07468112655580.0682076947158-2.30506820005E-60.2428628026160.010176923864-0.002169627380490.263006255705-0.01844343863180.282739913046147.19633133353.119558684721.0102277639
330.9680078537660.178439758519-0.0393818860766-0.0160453174909-0.1901521316690.493976932219-0.0581864678089-0.00994063058965-0.0107469016255-0.003031739191890.0238693841034-0.0154291001194-0.0407329753850.0682349703961-0.0001761136000860.197458638427-0.03191417274230.0005186778013590.1846096561130.02191670340230.203400193659153.2896910772.44773769956-20.6831175336
340.900085199060.167954802406-0.1613557148390.0883224765443-0.06531094222370.494622706229-0.09631182956710.137454454383-0.0840833262793-0.008344621105990.0123421190257-0.06345636733590.02773387213430.02304949089755.72293967683E-50.20417434915-0.04687976938460.001302181321850.1934693999870.01507319081480.211456099148145.726962912-4.6425680763-29.8028714216
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 84 )
2X-RAY DIFFRACTION2chain 'A' and (resid 85 through 192 )
3X-RAY DIFFRACTION3chain 'A' and (resid 193 through 267 )
4X-RAY DIFFRACTION4chain 'B' and (resid 3 through 99 )
5X-RAY DIFFRACTION5chain 'B' and (resid 100 through 192 )
6X-RAY DIFFRACTION6chain 'B' and (resid 193 through 234 )
7X-RAY DIFFRACTION7chain 'B' and (resid 235 through 267 )
8X-RAY DIFFRACTION8chain 'C' and (resid 2 through 84 )
9X-RAY DIFFRACTION9chain 'C' and (resid 85 through 121 )
10X-RAY DIFFRACTION10chain 'C' and (resid 122 through 150 )
11X-RAY DIFFRACTION11chain 'C' and (resid 151 through 192 )
12X-RAY DIFFRACTION12chain 'C' and (resid 193 through 234 )
13X-RAY DIFFRACTION13chain 'C' and (resid 235 through 267 )
14X-RAY DIFFRACTION14chain 'D' and (resid 2 through 99 )
15X-RAY DIFFRACTION15chain 'D' and (resid 100 through 192 )
16X-RAY DIFFRACTION16chain 'D' and (resid 193 through 234 )
17X-RAY DIFFRACTION17chain 'D' and (resid 235 through 267 )
18X-RAY DIFFRACTION18chain 'E' and (resid 3 through 192 )
19X-RAY DIFFRACTION19chain 'E' and (resid 193 through 234 )
20X-RAY DIFFRACTION20chain 'E' and (resid 235 through 267 )
21X-RAY DIFFRACTION21chain 'F' and (resid -2 through 192 )
22X-RAY DIFFRACTION22chain 'F' and (resid 193 through 267 )
23X-RAY DIFFRACTION23chain 'G' and (resid 4 through 16 )
24X-RAY DIFFRACTION24chain 'G' and (resid 17 through 56 )
25X-RAY DIFFRACTION25chain 'G' and (resid 57 through 71 )
26X-RAY DIFFRACTION26chain 'G' and (resid 72 through 84 )
27X-RAY DIFFRACTION27chain 'G' and (resid 85 through 121 )
28X-RAY DIFFRACTION28chain 'G' and (resid 122 through 150 )
29X-RAY DIFFRACTION29chain 'G' and (resid 151 through 192 )
30X-RAY DIFFRACTION30chain 'G' and (resid 193 through 216 )
31X-RAY DIFFRACTION31chain 'G' and (resid 217 through 246 )
32X-RAY DIFFRACTION32chain 'G' and (resid 247 through 267 )
33X-RAY DIFFRACTION33chain 'H' and (resid 2 through 192 )
34X-RAY DIFFRACTION34chain 'H' and (resid 193 through 267 )

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