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- PDB-7kqn: Ternary complex of TERT (telomerase reverse transcriptase) with R... -

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Basic information

Entry
Database: PDB / ID: 7kqn
TitleTernary complex of TERT (telomerase reverse transcriptase) with RNA template, DNA primer, an incoming dGTP and a downstream hybrid duplex
Components
  • DNA (5'-D(*GP*CP*AP*CP*CP*TP*G)-3')
  • DNA (5'-D(*TP*TP*CP*TP*TP*TP*G)-3')
  • RNA (5'-R(*CP*AP*AP*AP*GP*AP*AP*AP*UP*CP*CP*AP*GP*GP*UP*GP*CP*A)-3')
  • Telomerase reverse transcriptase
KeywordsTRANSFERASE/RNA/DNA / RAP / Repeat Addition Processivity / DNA hairpin / RNA template / telomere / TBE / Template-Boundary Element / TRANSFERASE / TRANSFERASE-RNA-DNA complex
Function / homology
Function and homology information


telomerase activity / telomere maintenance / RNA-directed DNA polymerase / chromosome, telomeric region / DNA binding / metal ion binding / nucleus
Similarity search - Function
Telomerase reverse transcriptase, thumb DNA-binding domain / Telomerase reverse transcriptase thumb DNA binding domain / Telomerase reverse transcriptase / Telomerase ribonucleoprotein complex - RNA-binding domain / Telomerase ribonucleoprotein complex - RNA binding domain / Reverse transcriptase domain / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase (RT) catalytic domain profile. / DNA/RNA polymerase superfamily
Similarity search - Domain/homology
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / DI(HYDROXYETHYL)ETHER / DNA / RNA / RNA (> 10) / Telomerase reverse transcriptase
Similarity search - Component
Biological speciesTribolium castaneum (red flour beetle)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.02 Å
AuthorsChoi, W.S. / Weng, P.J. / Yang, W.
Citation
Journal: Proc.Natl.Acad.Sci.USA / Year: 2022
Title: Flexibility of telomerase in binding the RNA template and DNA telomeric repeat.
Authors: Choi, W.S. / Weng, P.J. / Yang, W.
#1: Journal: Nat Struct Mol Biol / Year: 2015
Title: A DNA-hairpin model for repeat-addition processivity in telomere synthesis.
Authors: Yang, W. / Lee, Y.S.
History
DepositionNov 17, 2020Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 23, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Telomerase reverse transcriptase
B: RNA (5'-R(*CP*AP*AP*AP*GP*AP*AP*AP*UP*CP*CP*AP*GP*GP*UP*GP*CP*A)-3')
C: DNA (5'-D(*GP*CP*AP*CP*CP*TP*G)-3')
D: Telomerase reverse transcriptase
E: RNA (5'-R(*CP*AP*AP*AP*GP*AP*AP*AP*UP*CP*CP*AP*GP*GP*UP*GP*CP*A)-3')
F: DNA (5'-D(*GP*CP*AP*CP*CP*TP*G)-3')
I: DNA (5'-D(*TP*TP*CP*TP*TP*TP*G)-3')
J: DNA (5'-D(*TP*TP*CP*TP*TP*TP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)164,16937
Polymers161,1688
Non-polymers3,00129
Water23,4921304
1
A: Telomerase reverse transcriptase
B: RNA (5'-R(*CP*AP*AP*AP*GP*AP*AP*AP*UP*CP*CP*AP*GP*GP*UP*GP*CP*A)-3')
C: DNA (5'-D(*GP*CP*AP*CP*CP*TP*G)-3')
J: DNA (5'-D(*TP*TP*CP*TP*TP*TP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)82,24919
Polymers80,5844
Non-polymers1,66515
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
D: Telomerase reverse transcriptase
E: RNA (5'-R(*CP*AP*AP*AP*GP*AP*AP*AP*UP*CP*CP*AP*GP*GP*UP*GP*CP*A)-3')
F: DNA (5'-D(*GP*CP*AP*CP*CP*TP*G)-3')
I: DNA (5'-D(*TP*TP*CP*TP*TP*TP*G)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)81,92018
Polymers80,5844
Non-polymers1,33614
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)71.860, 164.040, 95.360
Angle α, β, γ (deg.)90.000, 111.914, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 1 through 5 or resid 7...
d_2ens_1(chain "D" and (resid 1 through 5 or resid 7...
d_1ens_2chain "B"
d_2ens_2(chain "E" and ((resid 1 and (name C5" or name...
d_1ens_3chain "C"
d_2ens_3chain "F"
d_1ens_4chain "I"
d_2ens_4chain "J"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1METTYRA1 - 5
d_12ens_1LEULYSA7 - 10
d_13ens_1ARGVALA13 - 20
d_14ens_1LYSLYSA23 - 40
d_15ens_1LYSLYSA42 - 44
d_16ens_1PROSERA46 - 160
d_17ens_1VALLYSA162 - 167
d_18ens_1METVALA169 - 175
d_19ens_1ASPLYSA177 - 190
d_110ens_1ASPASPA192
d_111ens_1PHEPHEA195 - 211
d_112ens_1LEULEUA213 - 214
d_113ens_1SERTRPA217 - 237
d_114ens_1GLUPHEA240 - 299
d_115ens_1LYSTYRA303 - 305
d_116ens_1TRPPHEA308 - 335
d_117ens_1ASNASPA338 - 340
d_118ens_1ASPASPA342 - 350
d_119ens_1TYRPROA353 - 360
d_120ens_1LYSLEUA363 - 370
d_121ens_1LYSTYRA372 - 375
d_122ens_1VALVALA377
d_123ens_1PROALAA380 - 442
d_124ens_1PHEGLNA444 - 470
d_125ens_1VALILEA475 - 484
d_126ens_1VALTYRA487 - 489
d_127ens_1PHELEUA492 - 515
d_128ens_1SERVALA517 - 519
d_129ens_1ALAPHEA521 - 527
d_130ens_1LYSTYRA529 - 538
d_131ens_1LYSTHRA540 - 562
d_132ens_1ILESERA564 - 574
d_133ens_1GLUILEA576 - 610
d_21ens_1METTYRD1 - 5
d_22ens_1LEULYSD8 - 11
d_23ens_1ARGVALD13 - 20
d_24ens_1LYSLYSD23 - 40
d_25ens_1LYSLYSD42 - 44
d_26ens_1PROSERD46 - 160
d_27ens_1VALLYSD162 - 167
d_28ens_1METVALD169 - 175
d_29ens_1ASPLYSD177 - 190
d_210ens_1ASPASPD192
d_211ens_1PHEPHED194 - 210
d_212ens_1LEULEUD212 - 213
d_213ens_1SERTRPD215 - 235
d_214ens_1GLUPHED237 - 296
d_215ens_1LYSTYRD300 - 302
d_216ens_1TRPPHED304 - 331
d_217ens_1ASNASPD333 - 335
d_218ens_1ASPASPD337 - 345
d_219ens_1TYRPROD347 - 354
d_220ens_1LYSLEUD357 - 364
d_221ens_1LYSTYRD367 - 370
d_222ens_1VALVALD372
d_223ens_1PROALAD374 - 436
d_224ens_1PHEGLND439 - 465
d_225ens_1VALILED469 - 478
d_226ens_1VALTYRD481 - 483
d_227ens_1PHELEUD485 - 508
d_228ens_1SERVALD511 - 513
d_229ens_1ALAPHED515 - 521
d_230ens_1LYSTYRD523 - 532
d_231ens_1LYSTHRD534 - 556
d_232ens_1ILESERD558 - 568
d_233ens_1GLUILED570 - 604
d_11ens_2CAB
d_21ens_2CAE
d_11ens_3DGDGC
d_21ens_3DGDGF
d_11ens_4DTDGG
d_21ens_4DTDGH

NCS ensembles :
ID
ens_1
ens_2
ens_3
ens_4

NCS oper:
IDCodeMatrixVector
1given(0.999879544833, 0.0117391896391, -0.0101531891962), (0.0104689523455, -0.993053210658, -0.117199495901), (-0.0114584842377, 0.117079085362, -0.993056489284)0.650153511874, -80.61454842, -0.145004320035
2given(0.998949046267, 0.0455722275048, 0.0048964316705), (0.0458064523523, -0.996362197272, -0.0718619563484), (0.00160370999436, 0.0720107209211, -0.997402568769)1.46209886281, -81.5727748322, -2.03593416054
3given(0.99956041757, -0.0172256545766, 0.0241298249533), (-0.0148428180616, -0.995302652791, -0.0956677589281), (0.0256644185568, 0.0952675504605, -0.995120812489)-0.0421185787943, -80.2879438569, -1.61770364728
4given(0.998576016168, 0.048221575284, 0.0228170903432), (0.0488712173855, -0.998389024416, -0.0288263774503), (0.0213902792374, 0.0299004281371, -0.999323981675)3.03014689191, -85.718506636, -3.07247515612

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Components

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DNA chain , 2 types, 4 molecules CFIJ

#3: DNA chain DNA (5'-D(*GP*CP*AP*CP*CP*TP*G)-3')


Mass: 2098.399 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Tribolium castaneum (red flour beetle)
#4: DNA chain DNA (5'-D(*TP*TP*CP*TP*TP*TP*G)-3')


Mass: 2094.394 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Tribolium castaneum (red flour beetle)

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Protein / RNA chain , 2 types, 4 molecules ADBE

#1: Protein Telomerase reverse transcriptase


Mass: 70588.719 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Tribolium castaneum (red flour beetle) / Gene: TERT, TcasGA2_TC010963 / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta2 (DE3) / References: UniProt: Q0QHL8, RNA-directed DNA polymerase
#2: RNA chain RNA (5'-R(*CP*AP*AP*AP*GP*AP*AP*AP*UP*CP*CP*AP*GP*GP*UP*GP*CP*A)-3')


Mass: 5802.571 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Tribolium castaneum (red flour beetle)

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Non-polymers , 6 types, 1333 molecules

#5: Chemical...
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 21 / Source method: obtained synthetically / Formula: C2H6O2
#6: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#7: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C4H10O3
#8: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca / Feature type: SUBJECT OF INVESTIGATION
#9: Chemical ChemComp-DGT / 2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE


Mass: 507.181 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O13P3 / Feature type: SUBJECT OF INVESTIGATION
#10: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1304 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.24 Å3/Da / Density % sol: 61.98 %
Crystal growTemperature: 294 K / Method: vapor diffusion, sitting drop
Details: 0.15 M Potassium Chloride, 0.05 M MES (pH 6.0), 2% PEG 8000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-BM / Wavelength: 1 Å
DetectorType: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jul 27, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.02→30 Å / Num. obs: 131028 / % possible obs: 97.9 % / Redundancy: 3.7 % / Biso Wilson estimate: 42.33 Å2 / CC1/2: 0.998 / Net I/σ(I): 10.55
Reflection shellResolution: 2.02→2.13 Å / Mean I/σ(I) obs: 1.07 / Num. unique obs: 9762 / CC1/2: 0.433

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Processing

Software
NameVersionClassification
PHENIX1.18.2_3874refinement
XDSdata reduction
XSCALEdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3KYL

3kyl


Resolution: 2.02→29.65 Å / SU ML: 0.226 / Cross valid method: FREE R-VALUE / σ(F): 1.25 / Phase error: 22.6202
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.1878 1007 0.77 %
Rwork0.1675 130002 -
obs0.1677 131009 98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 59.28 Å2
Refinement stepCycle: LAST / Resolution: 2.02→29.65 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9879 1323 186 1304 12692
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.009711989
X-RAY DIFFRACTIONf_angle_d1.228116469
X-RAY DIFFRACTIONf_chiral_restr0.07071811
X-RAY DIFFRACTIONf_plane_restr0.00831825
X-RAY DIFFRACTIONf_dihedral_angle_d20.73464629
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.767727991956
ens_2d_2BX-RAY DIFFRACTIONTorsion NCS0.676973963642
ens_3d_2CX-RAY DIFFRACTIONTorsion NCS0.260981586645
ens_4d_2DX-RAY DIFFRACTIONTorsion NCS0.51146032349
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.02-2.130.32671420.335718726X-RAY DIFFRACTION99.1
2.13-2.260.27371470.276518812X-RAY DIFFRACTION99.57
2.26-2.430.26341430.225518857X-RAY DIFFRACTION99.74
2.43-2.680.23671480.195618925X-RAY DIFFRACTION99.81
2.68-3.070.18231450.176518855X-RAY DIFFRACTION99.74
3.07-3.860.1751410.143518409X-RAY DIFFRACTION96.99
3.86-29.650.14841410.132317418X-RAY DIFFRACTION91.15
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.026304259510.02433105708770.1270275235641.331130200750.5022640299591.40410000884-0.0246330873319-0.781433582905-0.7138423311230.6104874810220.0842986920150.3987604729740.626607799614-0.4125549729930.006816813720350.776016624651-0.07700531738780.05592635677860.7651984295230.3126191299960.66588615555715.2274672595-52.300737174732.5141668368
21.1781105602-0.8511428169320.8131648278881.11543534345-0.7937429810051.0895124815-0.0593124237913-0.441347147229-0.353071115990.1516727807250.2655906444860.30701406320.109741891225-0.3701798039541.16950013316E-80.529517275125-0.03013390126520.06797266431520.6325556305020.08602413506450.5358592552693.13711715528-30.308418337619.6227417071
31.91416933628-0.462604263190.2099302598211.940285928730.04214885308220.660920709459-0.0646487630426-0.03713170461340.2282059797450.07310301823150.05098166198490.0287395351496-0.0373695290583-0.115545993307-0.0007943133010210.3020984608990.00166924556730.002257369070990.312523605087-0.01594232977240.2670204467113.7803479445-14.71980590726.73352395813
43.358700129590.287743101059-0.06655057648191.92043687122-0.6738661174712.545170532910.0964662993928-0.312211473737-0.302645202960.144504455988-0.170311679382-0.8275618240090.2114909958270.284537059197-0.001290070136750.3626767759460.0188467935465-0.05660935290920.333977152585-0.00521512617890.58196656772338.615962873-31.300893010110.0763026302
50.04732241241580.113090455145-0.08088471352120.225563553627-0.2723382067350.1241364545960.108282703265-1.61944415176-0.02374854711520.05270547101580.3756592540620.7593706953150.493453542865-0.4299459809110.1045305638380.7259318368180.01406297006790.2958329744861.343777401030.481998516780.796786776488-3.07488232474-38.542000877830.4298760348
60.008575008713260.00698482450377-0.02071841119650.193232878681-0.07705241959140.06178357605250.1846076027590.21959643409-0.393808514217-0.078999693182-0.1825514759520.3578727782480.134379785802-0.3045656726632.19462652584E-70.3425286333570.015354195860.02800275121660.339644238957-0.0178904016740.3191186903914.9117012825-28.5060753649-0.151060698979
70.0303358871688-0.00568557873887-0.06235882134520.01105319064620.09868599516160.0495008138089-0.163778012201-0.05395449671290.07743588471430.528687384677-0.03684286007520.7919349433790.386158755934-0.1178490809251.37704511639E-60.3530314562030.01938878120410.06688408022020.297777205372-0.04813161401320.35487964537816.2617785578-29.6614494236.06122274075
81.18642769612-0.394263995604-0.3683264829650.807533144865-0.1604437401370.770602542134-0.06193258507391.234738099110.793648360246-0.416720933508-0.219080826027-0.0154795057611-0.541910711761-0.110447681007-0.06169492000990.8037919900820.0173291937196-0.09407219737421.102861211540.4177643977940.62164617348914.5150509841-31.5728350455-37.8814116392
91.520788272621.1326559612-1.037696465371.28220577895-0.9977909518771.25230393191-0.0667630564580.4864838763380.178149964974-0.1301702003490.1859799798580.345673953199-0.115904107405-0.152701019898-2.8028354389E-80.5499209327090.0667411479903-0.06959015644010.680030150779-0.01075448103210.5324434717691.51270326714-57.0713807122-21.5189214432
103.69530730531-0.06928083226640.1196081277921.98762502815-0.2245306909930.921649031601-0.06035963405040.0374323169692-0.2577107203930.0259654255240.0350023284540.06868900950530.0875288095451-0.123125823721-0.0009485568682410.324367195414-0.01299399270490.02243819647140.30008652572-0.02504815477650.32307295878210.4718336972-72.4242058445-7.25590836755
113.18147320812-0.05511322594870.316787076932.9008791692-0.7998051794851.81586072303-0.08442343361690.4803449980140.222120721061-0.166011314768-0.118031282911-0.734219904511-0.1391884352540.293695831714-0.001433667537210.363525569753-0.04152435579710.03367178244460.3788850008130.001581844479030.43608579403835.2458756608-49.4809793178-14.0482663396
120.0145186184358-0.0752428123272-0.08167632214870.3467126755060.3608084186230.369378805460.03360178037570.49476358829-0.15900820574-0.9307612919560.6832423404240.00412669805133-0.1651201252740.3780214407330.8291505740470.743489474822-0.192786978845-0.3888300520641.332865160520.4980938268910.789695196586-6.93786842288-41.1047136688-44.3896758724
13-0.008330903236260.003567823928860.0506658964774-0.004868221948060.05856817653510.04655693960180.1404489831851.31052229249-0.0588804616962-0.2840097582090.3525873055440.629942647692-0.370210853718-0.2907463486860.00649255254020.638823294090.0505999519864-0.03684090048780.8916040779890.4067976540780.8308716983180.20550997843-50.0463453671-26.5400349836
14-0.00693499738120.006363887136450.04162515722790.258841885113-0.1490205753240.2394749083460.0410077295204-0.2268378400670.2048857956090.305788875802-0.2652899310880.767466259443-0.00728745790208-0.399922749777-0.0003640433084920.347806248046-0.00745756093213-0.0006633220921020.328310057861-0.07531803689210.4028572519915.1544751632-52.3314605201-3.75333553976
150.2399810182470.0638511124503-0.09224390884390.05765773214280.008990386618530.174136443125-0.002110339852710.3556777409270.04616985376-0.543000427422-0.4103865518151.19227135221-0.43681950045-0.7130987844720.0003218621156740.4113518374870.0376535540408-0.06643431076430.361585650451-0.1204269389680.44448992599116.8769947932-51.5750443476-10.0570882301
160.1196955568460.105404560874-0.03723991257170.1337699445610.1269152969560.2524703107740.2793469990530.776840004655-0.8782451930250.189654642009-0.3551635587710.581721249829-0.38493209074-0.115899115042-1.28220961673E-60.9124363732420.102544074892-0.3175963735061.345384947220.2752291058381.15721768975-10.9231113468-46.0579902274-40.0758947872
170.04345495326570.0144571417702-0.01902626576760.03510960635050.07522405786280.120766862240.0585025295072-0.02392272630490.229510104889-0.2230457512-0.2627168960290.636786612608-0.0334005443666-0.300580331071-0.001393368408471.13865749188-0.1476254731070.3414686327091.738044615520.4061184911981.21515057348-11.0208195424-39.119915414435.377647675
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 110 )AA1 - 1101 - 111
22chain 'A' and (resid 111 through 223 )AA111 - 223112 - 226
33chain 'A' and (resid 224 through 459 )AA224 - 459227 - 469
44chain 'A' and (resid 460 through 596 )AA460 - 596470 - 610
55chain 'B' and (resid 1 through 10 )BB1 - 10
66chain 'B' and (resid 11 through 18 )BB11 - 18
77chain 'C' and (resid 1 through 7 )CC1 - 7
88chain 'D' and (resid 1 through 125 )DD1 - 1251 - 126
99chain 'D' and (resid 126 through 223 )DD126 - 223127 - 224
1010chain 'D' and (resid 224 through 403 )DD224 - 403225 - 407
1111chain 'D' and (resid 404 through 596 )DD404 - 596408 - 604
1212chain 'E' and (resid 1 through 5 )EE1 - 5
1313chain 'E' and (resid 6 through 10 )EE6 - 10
1414chain 'E' and (resid 11 through 18 )EE11 - 18
1515chain 'F' and (resid 1 through 7 )FF1 - 7
1616chain 'I' and (resid 1 through 7 )IG1 - 7
1717chain 'J' and (resid 1 through 7 )JH1 - 7

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