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- PDB-7k0a: Puromycin N-acetyltransferase in complex with acetylated puromyci... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7k0a | ||||||
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Title | Puromycin N-acetyltransferase in complex with acetylated puromycin and CoA | ||||||
![]() | Puromycin N-acetyltransferase | ||||||
![]() | TRANSFERASE / Enzyme acetyletransferase complex selection marker / ANTIBIOTIC | ||||||
Function / homology | ![]() Transferases; Acyltransferases / acetyltransferase activity / response to antibiotic Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Peat, T.S. / Caputo, A.T. / Newman, J. / Adams, T.E. | ||||||
![]() | ![]() Title: Structure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins. Authors: Caputo, A.T. / Eder, O.M. / Bereznakova, H. / Pothuis, H. / Ardevol, A. / Newman, J. / Nuttall, S. / Peat, T.S. / Adams, T.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 106.7 KB | Display | ![]() |
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PDB format | ![]() | 77 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 21.5 KB | Display | |
Data in CIF | ![]() | 29.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7k09SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Noncrystallographic symmetry (NCS) | NCS domain: (Details: Chains A B) |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 22581.490 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 6 types, 288 molecules ![](data/chem/img/VQ1.gif)
![](data/chem/img/COA.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/COA.gif)
![](data/chem/img/PEG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-SO4 / #6: Chemical | #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.79 Å3/Da / Density % sol: 55.94 % |
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Crystal grow | Temperature: 281 K / Method: vapor diffusion, sitting drop / pH: 8.26 Details: 9.9% (w/v) 1,6-hexanediol, 1.59 M ammonium sulfate, 7.4 % (v/v) polyethylene glycol 400, 0.1 M HEPES pH 8.26, 0.5 n-hexyl-b-D-glucopyranoside |
-Data collection
Diffraction |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Resolution: 2→70.75 Å / Num. obs: 33396 / % possible obs: 99.9 % / Redundancy: 17.2 % / CC1/2: 0.989 / Rmerge(I) obs: 0.376 / Rpim(I) all: 0.136 / Rrim(I) all: 0.4 / Χ2: 1.02 / Net I/σ(I): 7.7 | ||||||||||||||||||||
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 15.2 % / Mean I/σ(I) obs: 2.3 / Num. unique obs: 2460 / CC1/2: 0.857 / Rpim(I) all: 0.608 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7K09 Resolution: 2→42.488 Å / Cor.coef. Fo:Fc: 0.92 / Cor.coef. Fo:Fc free: 0.895 / SU B: 4.723 / SU ML: 0.126 / Cross valid method: FREE R-VALUE / ESU R: 0.187 / ESU R Free: 0.162 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.23 Å2
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Refinement step | Cycle: LAST / Resolution: 2→42.488 Å
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Refine LS restraints |
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LS refinement shell |
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