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TitleStructure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins.
Journal, issue, pagesSci Rep, Vol. 11, Page 5247-5247, Year 2021
Publish dateSep 3, 2020 (structure data deposition date)
AuthorsCaputo, A.T. / Eder, O.M. / Bereznakova, H. / Pothuis, H. / Ardevol, A. / Newman, J. / Nuttall, S. / Peat, T.S. / Adams, T.E.
External linksSci Rep / PubMed:33664348
MethodsX-ray diffraction
Resolution2 - 2.313 Å
Structure data

PDB-7k09:
Puromycin N-acetyltransferase in complex with acetyl-CoA
Method: X-RAY DIFFRACTION / Resolution: 2.313 Å

PDB-7k0a:
Puromycin N-acetyltransferase in complex with acetylated puromycin and CoA
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-ACO:
ACETYL COENZYME *A

ChemComp-HOH:
WATER

ChemComp-VQ1:
N-Acetylpuromycin

ChemComp-COA:
COENZYME A

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-SO4:
SULFATE ION

ChemComp-EDO:
1,2-ETHANEDIOL

Source
  • streptomyces alboniger (bacteria)
KeywordsTRANSFERASE / Enzyme acetyletransferase complex selection marker / ANTIBIOTIC

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