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- PDB-7fgu: H/D exchanged Hen egg-white lysozyme denatured in basic condition... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7fgu | ||||||
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Title | H/D exchanged Hen egg-white lysozyme denatured in basic conditions and refolded in solution | ||||||
![]() | Lysozyme C | ||||||
![]() | HYDROLASE / H/D exchange / denatured and refolded | ||||||
Function / homology | ![]() Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium ...Lactose synthesis / Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / defense response to Gram-negative bacterium / killing of cells of another organism / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Kita, A. / Morimoto, Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Hydrogen/Deuterium Exchange Behavior During Denaturing/Refolding Processes Determined in Tetragonal Hen Egg-White Lysozyme Crystals. Authors: Kita, A. / Morimoto, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 72.9 KB | Display | ![]() |
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PDB format | ![]() | 50.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 373.7 KB | Display | ![]() |
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Full document | ![]() | 373.8 KB | Display | |
Data in XML | ![]() | 4.6 KB | Display | |
Data in CIF | ![]() | 7.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7fg8C ![]() 7fgvC ![]() 6k8gS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 14331.160 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
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#2: Chemical | ChemComp-CL / |
#3: Chemical | ChemComp-NA / |
#4: Water | ChemComp-HOH / |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment |
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Sample preparation
Crystal | Density Matthews: 2.08 Å3/Da / Density % sol: 41 % Description: The entry contains friedel pairs in F_plus/minus columns and I_plus/minus columns |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 4.6 Details: NACL, SODIUM ACETATE-D3, ACETIC ACID-D4, 100% D2O, PH 4.6 |
-Data collection
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Radiation |
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Radiation wavelength |
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Reflection | Biso Wilson estimate: 23.04 Å2 / Entry-ID: 7FGU
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Reflection shell |
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Serial crystallography sample delivery | Method: fixed target |
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Processing
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Refinement | SU ML: 0.1628 / Cross valid method: FREE R-VALUE / Method to determine structure:
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Refinement step | Cycle: LAST / Resolution: 2→39.63 Å
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Refine LS restraints |
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LS refinement shell |
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