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Open data
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Basic information
Entry | Database: PDB / ID: 7fbv | ||||||
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Title | The solution structure of the second RRM domain of Matrin-3 | ||||||
![]() | Matrin-3 | ||||||
![]() | RNA BINDING PROTEIN / RRM / ALS/FTD / nuclear matrix protein / Structural Genomics / PSI-2 / Protein Structure Initiative / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | ![]() heart valve development / blastocyst formation / miRNA binding / ventricular septum development / post-transcriptional regulation of gene expression / activation of innate immune response / nuclear matrix / innate immune response / RNA binding / zinc ion binding ...heart valve development / blastocyst formation / miRNA binding / ventricular septum development / post-transcriptional regulation of gene expression / activation of innate immune response / nuclear matrix / innate immune response / RNA binding / zinc ion binding / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
![]() | Muto, Y. / Kobayashi, N. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
![]() | ![]() Title: 1 H, 13 C and 15 N resonance assignments and solution structures of the two RRM domains of Matrin-3. Authors: He, F. / Kuwasako, K. / Takizawa, M. / Takahashi, M. / Tsuda, K. / Nagata, T. / Watanabe, S. / Tanaka, A. / Kobayashi, N. / Kigawa, T. / Guntert, P. / Shirouzu, M. / Yokoyama, S. / Muto, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 691.8 KB | Display | ![]() |
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PDB format | ![]() | 579.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 466.3 KB | Display | ![]() |
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Full document | ![]() | 561.4 KB | Display | |
Data in XML | ![]() | 33.3 KB | Display | |
Data in CIF | ![]() | 58.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7fbrC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 12499.458 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Production host: Cell-free gateway cloning vector N-term 8xHis eGFP pCellFree_G03 (others) References: UniProt: Q8K310 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Sample state: isotropic / Type: 3D 13C,15N-SEPARATED NOESY SPECTRA |
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Sample preparation
Details | Type: solution Contents: 1 mM [U-99% 13C; U-99% 15N] RNA binding protein, 90% H2O/10% D2O Details: 1mM D-DTT;0.02% NaN3 were used to keep the protein condition Label: 13C,15N_sample / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1 mM / Component: RNA binding protein / Isotopic labeling: [U-99% 13C; U-99% 15N] |
Sample conditions | Details: 20mM D-Tris-HCL (PH7.0); 100mM NaCl; 1mM D-DTT;0.02% NaN3 Ionic strength: 100 mM / Label: conditions_1 / pH: 7 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with favorable non-bond energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |