+
Open data
-
Basic information
Entry | Database: PDB / ID: 7f0k | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of Kmp11 | ||||||
![]() | Kinetoplastid membrane protein KMP-11 | ||||||
![]() | LIPID BINDING PROTEIN / Kmp11 / kinetoplastid / membrane BINDING PROTEIN | ||||||
Function / homology | Kinetoplastid membrane protein 11 / Kinetoplastid membrane protein 11 / defense response / microtubule / positive regulation of cell population proliferation / cytoplasm / Kinetoplastid membrane protein KMP-11![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mo, X. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Structural insight into dynamic characteristic of the polymerized forms of kinetoplastid membrane protein-11 Authors: Mo, X. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 51.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 33.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 431.7 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 432.3 KB | Display | |
Data in XML | ![]() | 7.5 KB | Display | |
Data in CIF | ![]() | 9.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Unit cell |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
|
-
Components
#1: Protein | Mass: 11333.037 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: MHOM/CI/86/DAL972 Gene: TbgDal_IX8640, TbgDal_IX8650, TbgDal_IX8660, TbgDal_IX8670 Production host: ![]() ![]() Has ligand of interest | N | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.15 Å3/Da / Density % sol: 42.86 % |
---|---|
Crystal grow | Temperature: 280 K / Method: vapor diffusion, hanging drop / Details: Ammonium Sulfate, Polyethylene glycol 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 130 mm / Detector: CCD / Date: May 14, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 3.5→50 Å / Num. obs: 2583 / % possible obs: 99.16 % / Redundancy: 3.1 % / Biso Wilson estimate: 47.8 Å2 / CC1/2: 0.8 / Net I/σ(I): 9 |
Reflection shell | Resolution: 3.5→3.625 Å / Num. unique obs: 236 / CC1/2: 0.8 |
-
Processing
Software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
| ||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 46.87 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.5→31.32 Å
| ||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||
LS refinement shell | Highest resolution: 3.5 Å
|