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- PDB-5j6p: Crystal Structure of Mis18(17-118) from Schizosaccharomyces pombe -
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Open data
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Basic information
Entry | Database: PDB / ID: 5j6p | ||||||
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Title | Crystal Structure of Mis18(17-118) from Schizosaccharomyces pombe | ||||||
![]() | Kinetochore protein mis18 | ||||||
![]() | CELL CYCLE / Mis18 / centromere assembly | ||||||
Function / homology | ![]() CENP-A recruiting complex / CENP-A containing chromatin assembly / mitotic sister chromatid segregation / chromosome, centromeric region / chromosome segregation / kinetochore / cell division / chromatin / zinc ion binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Wang, C. / Shao, C. / Zhang, M. / Zhang, X. / Zang, J. | ||||||
![]() | ![]() Title: Crystal Structure of Mis18(17-118) from Schizosaccharomyces pombe Authors: Shao, C. / Wang, C. / Zhang, M. / Zhang, X. / Zang, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 74.1 KB | Display | ![]() |
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PDB format | ![]() | 55.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 421 KB | Display | ![]() |
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Full document | ![]() | 423.9 KB | Display | |
Data in XML | ![]() | 8.1 KB | Display | |
Data in CIF | ![]() | 11.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 12796.233 Da / Num. of mol.: 3 / Fragment: UNP RESIDUES 18-118 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: 972 / ATCC 24843 / Gene: mis18, SPCC970.12 / Production host: ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.17 Å3/Da / Density % sol: 70.51 % / Description: rhombus |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.2M (NH4)2SO4, 20% PEG 3350, 0.4M 1,6-Hexanediol, 0.1M Tris pH 8.5, 4% Dioxane |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Mar 25, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97915 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→60.39 Å / Num. obs: 18911 / % possible obs: 99.8 % / Redundancy: 10.6 % / Rmerge(I) obs: 0.095 / Net I/σ(I): 14.9 |
Reflection shell | Resolution: 2.6→2.74 Å / Redundancy: 10.5 % / Rmerge(I) obs: 0.506 / Mean I/σ(I) obs: 4.8 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 85.122 Å2
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Refinement step | Cycle: 1 / Resolution: 2.6→46.66 Å
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Refine LS restraints |
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