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Open data
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Basic information
Entry | Database: PDB / ID: 7ewc | ||||||
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Title | Mycobacterium tuberculosis HigA2 (Form I) | ||||||
![]() | Putative antitoxin HigA2 | ||||||
![]() | DNA BINDING PROTEIN / Antitoxin / HigA2 | ||||||
Function / homology | HigA2-like, helix-turn-helix domain / Helix-turn-helix domain / Helix-turn-helix XRE-family like proteins / Cro/C1-type HTH domain profile. / Cro/C1-type helix-turn-helix domain / Lambda repressor-like, DNA-binding domain superfamily / DNA binding / Putative antitoxin HigA2![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kim, H.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Chasing the structural diversity of the transcription regulator Mycobacterium tuberculosis HigA2. Authors: Richardson, W. / Kang, G.W. / Lee, H.J. / Kwon, K.M. / Kim, S. / Kim, H.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.6 KB | Display | ![]() |
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PDB format | ![]() | 48 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7ewdC ![]() 7eweC ![]() 1y7yS ![]() 2a6cS ![]() 3b7hS ![]() 3kxaS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 11309.927 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: higA2, Rv2021c, RVBD_2021c, LH57_11010, P425_02092 / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.73 Å3/Da / Density % sol: 28.89 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 11% (w/v) PEK 20000, 0.1M MES pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Jul 3, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.05→40 Å / Num. obs: 20447 / % possible obs: 99.8 % / Redundancy: 6 % / CC1/2: 0.99 / Rmerge(I) obs: 0.079 / Net I/σ(I): 29 |
Reflection shell | Resolution: 2.05→2.09 Å / Num. unique obs: 969 / CC1/2: 0.984 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1Y7Y, 3B7H, 3KXA, 2A6C Resolution: 2.05→35.22 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.924 / SU B: 4.201 / SU ML: 0.116 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.194 / ESU R Free: 0.172 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 95.22 Å2 / Biso mean: 35.407 Å2 / Biso min: 12.96 Å2
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Refinement step | Cycle: final / Resolution: 2.05→35.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.05→2.103 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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