[English] 日本語
Yorodumi- PDB-7elj: Prion Derived Tetrapeptide Stabilizes Thermolabile Insulin via Co... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7elj | ||||||
---|---|---|---|---|---|---|---|
Title | Prion Derived Tetrapeptide Stabilizes Thermolabile Insulin via Conformational Trapping | ||||||
Components |
| ||||||
Keywords | HORMONE / Insulin / Stabilization / Amyloid | ||||||
Function / homology | Function and homology information estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate ...estradiol secretion / negative regulation of lactation / positive regulation of blood circulation / glucose import in response to insulin stimulus / positive regulation of cell maturation / positive regulation of lactation / response to L-arginine / positive regulation of mammary gland epithelial cell proliferation / negative regulation of appetite / response to butyrate / feeding behavior / response to growth hormone / response to food / positive regulation of Rho protein signal transduction / positive regulation of peptide hormone secretion / protein secretion / negative regulation of lipid catabolic process / response to glucose / response to nutrient levels / positive regulation of protein secretion / insulin receptor binding / positive regulation of insulin secretion / hormone activity / glucose metabolic process / glucose homeostasis / response to heat / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of gene expression / negative regulation of apoptotic process / extracellular space / identical protein binding Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) Homo sapiens (human) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Authors | Banerjee, N. | ||||||
Citation | Journal: Iscience / Year: 2021 Title: Prion-derived tetrapeptide stabilizes thermolabile insulin via conformational trapping. Authors: Mukherjee, M. / Das, D. / Sarkar, J. / Banerjee, N. / Jana, J. / Bhat, J. / Reddy G, J. / Bharatam, J. / Chattopadhyay, S. / Chatterjee, S. / Chakrabarti, P. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7elj.cif.gz | 140.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7elj.ent.gz | 116.1 KB | Display | PDB format |
PDBx/mmJSON format | 7elj.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7elj_validation.pdf.gz | 399.1 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7elj_full_validation.pdf.gz | 485.3 KB | Display | |
Data in XML | 7elj_validation.xml.gz | 11.7 KB | Display | |
Data in CIF | 7elj_validation.cif.gz | 17.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/el/7elj ftp://data.pdbj.org/pub/pdb/validation_reports/el/7elj | HTTPS FTP |
-Related structure data
Similar structure data | |
---|---|
Other databases |
|
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 2339.645 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 |
---|---|
#2: Protein/peptide | Mass: 3403.927 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P01317 |
#3: Protein/peptide | Mass: 600.687 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
|
-Sample preparation
Details | Type: solution Contents: 1 mM Proton Bovine Insulin, 1 mM Proton-15N, 13C Peptide, 90% H2O/10% D2O Label: Insulin / Solvent system: 90% H2O/10% D2O | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample |
| ||||||||||||
Sample conditions | Ionic strength: 1 mM / Label: Condition_1 / pH: 2.6 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
|
---|
-Processing
NMR software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: distance geometry / Software ordinal: 1 | ||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 10 / Conformers submitted total number: 8 |