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Open data
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Basic information
Entry | Database: PDB / ID: 7ej2 | ||||||
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Title | human voltage-gated potassium channel KV1.3 H451N mutant | ||||||
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![]() | MEMBRANE PROTEIN / potassium channel / complex / C-type inactivation | ||||||
Function / homology | ![]() pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / corpus callosum development / voltage-gated monoatomic ion channel activity / aldo-keto reductase (NADPH) activity / Voltage gated Potassium channels / juxtaparanode region of axon / regulation of potassium ion transmembrane transport ...pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / corpus callosum development / voltage-gated monoatomic ion channel activity / aldo-keto reductase (NADPH) activity / Voltage gated Potassium channels / juxtaparanode region of axon / regulation of potassium ion transmembrane transport / delayed rectifier potassium channel activity / outward rectifier potassium channel activity / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / optic nerve development / voltage-gated potassium channel activity / calyx of Held / tertiary granule membrane / potassium channel regulator activity / specific granule membrane / potassium ion transmembrane transport / voltage-gated potassium channel complex / extrinsic component of cytoplasmic side of plasma membrane / potassium ion transport / protein homooligomerization / cytoplasmic side of plasma membrane / presynaptic membrane / postsynaptic membrane / transmembrane transporter binding / cytoskeleton / membrane raft / axon / glutamatergic synapse / synapse / Neutrophil degranulation / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.3 Å | ||||||
![]() | Liu, S. / Zhao, Y. / Tian, C. | ||||||
![]() | ![]() Title: Structures of wild-type and H451N mutant human lymphocyte potassium channel K1.3. Authors: Sanling Liu / Yue Zhao / Hao Dong / Liang Xiao / Yong Zhang / Yuqin Yang / Seow Theng Ong / K George Chandy / Longhua Zhang / Changlin Tian / ![]() ![]() | ||||||
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Structure visualization
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 489.1 KB | Display | ![]() |
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PDB format | ![]() | 383.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.1 MB | Display | ![]() |
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Full document | ![]() | 1.1 MB | Display | |
Data in XML | ![]() | 78.2 KB | Display | |
Data in CIF | ![]() | 118.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 31149MC ![]() 7ej1C M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 41049.152 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q13303, Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor #2: Protein | Mass: 63882.434 Da / Num. of mol.: 4 / Mutation: H451N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Chemical | ChemComp-NAP / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Human KV1.3 H451N-KVb2.1 / Type: COMPLEX / Entity ID: #1-#2 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 62 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.3 Å / Num. of particles: 79338 / Symmetry type: POINT | ||||||||||||||||||||||||
Refinement | Cross valid method: NONE Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2 | ||||||||||||||||||||||||
Displacement parameters | Biso mean: 101.5 Å2 | ||||||||||||||||||||||||
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