+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-31148 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | human voltage-gated potassium channel KV1.3 | |||||||||
Map data | ||||||||||
Sample |
| |||||||||
Function / homology | Function and homology information pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / delayed rectifier potassium channel activity / corpus callosum development / voltage-gated monoatomic ion channel activity / aldo-keto reductase (NADPH) activity / Voltage gated Potassium channels / outward rectifier potassium channel activity ...pinceau fiber / regulation of action potential / NADPH oxidation / regulation of protein localization to cell surface / delayed rectifier potassium channel activity / corpus callosum development / voltage-gated monoatomic ion channel activity / aldo-keto reductase (NADPH) activity / Voltage gated Potassium channels / outward rectifier potassium channel activity / juxtaparanode region of axon / optic nerve development / regulation of potassium ion transmembrane transport / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / voltage-gated potassium channel activity / tertiary granule membrane / calyx of Held / potassium channel regulator activity / specific granule membrane / voltage-gated potassium channel complex / potassium ion transmembrane transport / extrinsic component of cytoplasmic side of plasma membrane / protein homooligomerization / potassium ion transport / cytoplasmic side of plasma membrane / presynaptic membrane / postsynaptic membrane / transmembrane transporter binding / cytoskeleton / membrane raft / axon / glutamatergic synapse / synapse / Neutrophil degranulation / membrane / plasma membrane / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.2 Å | |||||||||
Authors | Liu S / Zhao Y / Tian C | |||||||||
Citation | Journal: Cell Discov / Year: 2021 Title: Structures of wild-type and H451N mutant human lymphocyte potassium channel K1.3. Authors: Sanling Liu / Yue Zhao / Hao Dong / Liang Xiao / Yong Zhang / Yuqin Yang / Seow Theng Ong / K George Chandy / Longhua Zhang / Changlin Tian / | |||||||||
History |
|
-Structure visualization
Movie |
Movie viewer |
---|---|
Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_31148.map.gz | 7.3 MB | EMDB map data format | |
---|---|---|---|---|
Header (meta data) | emd-31148-v30.xml emd-31148.xml | 10.9 KB 10.9 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_31148_fsc.xml | 10.7 KB | Display | FSC data file |
Images | emd_31148.png | 98.5 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-31148 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-31148 | HTTPS FTP |
-Related structure data
Related structure data | 7ej1MC 7ej2C M: atomic model generated by this map C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
---|---|
Related items in Molecule of the Month |
-Map
File | Download / File: emd_31148.map.gz / Format: CCP4 / Size: 103 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Voxel size | X=Y=Z: 1.014 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
|
-Supplemental data
-Sample components
-Entire : Human potassium channel KV1.3/KVb2.1 complex
Entire | Name: Human potassium channel KV1.3/KVb2.1 complex |
---|---|
Components |
|
-Supramolecule #1: Human potassium channel KV1.3/KVb2.1 complex
Supramolecule | Name: Human potassium channel KV1.3/KVb2.1 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
-Macromolecule #1: Voltage-gated potassium channel subunit beta-2
Macromolecule | Name: Voltage-gated potassium channel subunit beta-2 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO EC number: Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 41.049152 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MYPESTTGSP ARLSLRQTGS PGMIYSTRYG SPKRQLQFYR NLGKSGLRVS CLGLGTWVTF GGQITDEMAE QLMTLAYDNG INLFDTAEV YAAGKAEVVL GNIIKKKGWR RSSLVITTKI FWGGKAETER GLSRKHIIEG LKASLERLQL EYVDVVFANR P DPNTPMEE ...String: MYPESTTGSP ARLSLRQTGS PGMIYSTRYG SPKRQLQFYR NLGKSGLRVS CLGLGTWVTF GGQITDEMAE QLMTLAYDNG INLFDTAEV YAAGKAEVVL GNIIKKKGWR RSSLVITTKI FWGGKAETER GLSRKHIIEG LKASLERLQL EYVDVVFANR P DPNTPMEE TVRAMTHVIN QGMAMYWGTS RWSSMEIMEA YSVARQFNLT PPICEQAEYH MFQREKVEVQ LPELFHKIGV GA MTWSPLA CGIVSGKYDS GIPPYSRASL KGYQWLKDKI LSEEGRRQQA KLKELQAIAE RLGCTLPQLA IAWCLRNEGV SSV LLGASN ADQLMENIGA IQVLPKLSSS IIHEIDSILG NKPYSKKDYR S |
-Macromolecule #2: Potassium voltage-gated channel subfamily A member 3
Macromolecule | Name: Potassium voltage-gated channel subfamily A member 3 / type: protein_or_peptide / ID: 2 / Number of copies: 4 / Enantiomer: LEVO |
---|---|
Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 63.90648 KDa |
Recombinant expression | Organism: Spodoptera frugiperda (fall armyworm) |
Sequence | String: MDERLSLLRS PPPPSARHRA HPPQRPASSG GAHTLVNHGY AEPAAGRELP PDMTVVPGDH LLEPEVADGG GAPPQGGCGG GGCDRYEPL PPSLPAAGEQ DCCGERVVIN ISGLRFETQL KTLCQFPETL LGDPKRRMRY FDPLRNEYFF DRNRPSFDAI L YYYQSGGR ...String: MDERLSLLRS PPPPSARHRA HPPQRPASSG GAHTLVNHGY AEPAAGRELP PDMTVVPGDH LLEPEVADGG GAPPQGGCGG GGCDRYEPL PPSLPAAGEQ DCCGERVVIN ISGLRFETQL KTLCQFPETL LGDPKRRMRY FDPLRNEYFF DRNRPSFDAI L YYYQSGGR IRRPVNVPID IFSEEIRFYQ LGEEAMEKFR EDEGFLREEE RPLPRRDFQR QVWLLFEYPE SSGPARGIAI VS VLVILIS IVIFCLETLP EFRDEKDYPA STSQDSFEAA GNSTSGSRAG ASSFSDPFFV VETLCIIWFS FELLVRFFAC PSK ATFSRN IMNLIDIVAI IPYFITLGTE LAERQGNGQQ AMSLAILRVI RLVRVFRIFK LSRHSKGLQI LGQTLKASMR ELGL LIFFL FIGVILFSSA VYFAEADDPT SGFSSIPDAF WWAVVTMTTV GYGDMHPVTI GGKIVGSLCA IAGVLTIALP VPVIV SNFN YFYHRETEGE EQSQYMHVGS CQHLSSSAEE LRKARSNSTL SKSEYMVIEE GGMNHSAFPQ TPFKTGNSTA TCTTNN NPN SCVNIKKIFT DV |
-Macromolecule #3: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
Macromolecule | Name: NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / type: ligand / ID: 3 / Number of copies: 4 / Formula: NAP |
---|---|
Molecular weight | Theoretical: 743.405 Da |
Chemical component information | ChemComp-NAP: |
-Experimental details
-Structure determination
Method | cryo EM |
---|---|
Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 8 |
---|---|
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
---|---|
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy |
Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Average electron dose: 62.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |