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- PDB-7e7h: Crystal Structure of a pseudooxynicotine amine oxidase Pnao from ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7e7h | ||||||
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Title | Crystal Structure of a pseudooxynicotine amine oxidase Pnao from Pseudomonas putida S16 | ||||||
![]() | Amino_oxidase domain-containing protein | ||||||
![]() | OXIDOREDUCTASE / amine oxidase | ||||||
Function / homology | Flavin amine oxidase / Amine oxidase / Flavin containing amine oxidoreductase / FAD/NAD(P)-binding domain superfamily / oxidoreductase activity / FLAVIN-ADENINE DINUCLEOTIDE / TRIETHYLENE GLYCOL / Amino_oxidase domain-containing protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tang, H.Z. | ||||||
![]() | ![]() Title: Crystal Structure of a pseudooxynicotine amine oxidase Pnao from Pseudomonas putida S16 Authors: Tang, H.Z. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 199.6 KB | Display | ![]() |
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PDB format | ![]() | 152.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 971.4 KB | Display | ![]() |
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Full document | ![]() | 980.6 KB | Display | |
Data in XML | ![]() | 37.6 KB | Display | |
Data in CIF | ![]() | 54.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6c71S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 52489.965 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 51.96 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.2 M Lithium sulfate, 25 % PEG 3350, BIS-TRIS ph 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 26, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 51082 / % possible obs: 100 % / Redundancy: 11.9 % / Biso Wilson estimate: 30.24 Å2 / Rmerge(I) obs: 0.187 / Rpim(I) all: 0.065 / Net I/σ(I): 18.5 |
Reflection shell | Resolution: 2.2→2.24 Å / Rmerge(I) obs: 0.643 / Mean I/σ(I) obs: 4.5 / Num. unique obs: 2538 / Rpim(I) all: 0.223 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6C71 Resolution: 2.2→34.83 Å / SU ML: 0.2026 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 20.5172 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 33.27 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→34.83 Å
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Refine LS restraints |
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LS refinement shell |
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