+Open data
-Basic information
Entry | Database: PDB / ID: 7d0i | ||||||||||||||||||||||||||||||
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Title | Cryo-EM structure of Schizosaccharomyces pombe Atg9 | ||||||||||||||||||||||||||||||
Components | Autophagy-related protein 9 | ||||||||||||||||||||||||||||||
Keywords | UNKNOWN FUNCTION / Autophagy / membrane protein | ||||||||||||||||||||||||||||||
Function / homology | Function and homology information Macroautophagy / : / phospholipid scramblase activity / protein localization to phagophore assembly site / phagophore assembly site membrane / autophagy of mitochondrion / fungal-type vacuole membrane / phagophore assembly site / autophagosome / macroautophagy ...Macroautophagy / : / phospholipid scramblase activity / protein localization to phagophore assembly site / phagophore assembly site membrane / autophagy of mitochondrion / fungal-type vacuole membrane / phagophore assembly site / autophagosome / macroautophagy / cytoplasmic vesicle membrane / autophagy / protein transport / membrane => GO:0016020 / Golgi membrane / endoplasmic reticulum membrane Similarity search - Function | ||||||||||||||||||||||||||||||
Biological species | Schizosaccharomyces pombe (fission yeast) | ||||||||||||||||||||||||||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3 Å | ||||||||||||||||||||||||||||||
Authors | Matoba, K. / Tsutsumi, A. / Kikkawa, M. / Noda, N.N. | ||||||||||||||||||||||||||||||
Funding support | Japan, 9items
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Citation | Journal: Nat Struct Mol Biol / Year: 2020 Title: Atg9 is a lipid scramblase that mediates autophagosomal membrane expansion. Authors: Kazuaki Matoba / Tetsuya Kotani / Akihisa Tsutsumi / Takuma Tsuji / Takaharu Mori / Daisuke Noshiro / Yuji Sugita / Norimichi Nomura / So Iwata / Yoshinori Ohsumi / Toyoshi Fujimoto / ...Authors: Kazuaki Matoba / Tetsuya Kotani / Akihisa Tsutsumi / Takuma Tsuji / Takaharu Mori / Daisuke Noshiro / Yuji Sugita / Norimichi Nomura / So Iwata / Yoshinori Ohsumi / Toyoshi Fujimoto / Hitoshi Nakatogawa / Masahide Kikkawa / Nobuo N Noda / Abstract: The molecular function of Atg9, the sole transmembrane protein in the autophagosome-forming machinery, remains unknown. Atg9 colocalizes with Atg2 at the expanding edge of the isolation membrane (IM) ...The molecular function of Atg9, the sole transmembrane protein in the autophagosome-forming machinery, remains unknown. Atg9 colocalizes with Atg2 at the expanding edge of the isolation membrane (IM), where Atg2 receives phospholipids from the endoplasmic reticulum (ER). Here we report that yeast and human Atg9 are lipid scramblases that translocate phospholipids between outer and inner leaflets of liposomes in vitro. Cryo-EM of fission yeast Atg9 reveals a homotrimer, with two connected pores forming a path between the two membrane leaflets: one pore, located at a protomer, opens laterally to the cytoplasmic leaflet; the other, at the trimer center, traverses the membrane vertically. Mutation of residues lining the pores impaired IM expansion and autophagy activity in yeast and abolished Atg9's ability to transport phospholipids between liposome leaflets. These results suggest that phospholipids delivered by Atg2 are translocated from the cytoplasmic to the luminal leaflet by Atg9, thereby driving autophagosomal membrane expansion. | ||||||||||||||||||||||||||||||
History |
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-Structure visualization
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Structure viewer | Molecule: MolmilJmol/JSmol |
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PDBx/mmCIF format | 7d0i.cif.gz | 510.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7d0i.ent.gz | 408.2 KB | Display | PDB format |
PDBx/mmJSON format | 7d0i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/d0/7d0i ftp://data.pdbj.org/pub/pdb/validation_reports/d0/7d0i | HTTPS FTP |
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-Related structure data
Related structure data | 30535MC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data |
-Links
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Deposited unit |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
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