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- PDB-7bv6: Crystal structure of the autophagic STX17/SNAP29/VAMP8 SNARE complex -

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Basic information

Entry
Database: PDB / ID: 7bv6
TitleCrystal structure of the autophagic STX17/SNAP29/VAMP8 SNARE complex
Components
  • (Synaptosomal-associated protein 29) x 2
  • Syntaxin-17
  • Vesicle-associated membrane protein 8
KeywordsSIGNALING PROTEIN / Autophagy / Synatxin 17 / GABARAP
Function / homology
Function and homology information


endoplasmic reticulum-Golgi intermediate compartment organization / mucin granule / negative regulation of secretion by cell / positive regulation of histamine secretion by mast cell / ciliary pocket membrane / smooth endoplasmic reticulum membrane / vesicle targeting / trans-Golgi Network Vesicle Budding / zymogen granule membrane / synaptic vesicle fusion to presynaptic active zone membrane ...endoplasmic reticulum-Golgi intermediate compartment organization / mucin granule / negative regulation of secretion by cell / positive regulation of histamine secretion by mast cell / ciliary pocket membrane / smooth endoplasmic reticulum membrane / vesicle targeting / trans-Golgi Network Vesicle Budding / zymogen granule membrane / synaptic vesicle fusion to presynaptic active zone membrane / Intra-Golgi traffic / mucus secretion / mitochondria-associated endoplasmic reticulum membrane contact site / autophagosome-lysosome fusion / autolysosome membrane / vesicle docking / chloride channel inhibitor activity / protein localization to phagophore assembly site / SNARE complex / SNAP receptor activity / vesicle fusion / COPII-coated ER to Golgi transport vesicle / COPII-mediated vesicle transport / syntaxin binding / azurophil granule membrane / Lysosome Vesicle Biogenesis / SNARE complex assembly / Golgi Associated Vesicle Biogenesis / autophagosome membrane docking / synaptic vesicle priming / endoplasmic reticulum-Golgi intermediate compartment / Golgi organization / exocytosis / autophagosome membrane / tertiary granule membrane / cilium assembly / autophagosome maturation / endoplasmic reticulum to Golgi vesicle-mediated transport / regulation of protein localization to plasma membrane / specific granule membrane / endoplasmic reticulum-Golgi intermediate compartment membrane / endomembrane system / autophagosome / secretory granule membrane / SNARE binding / intracellular protein transport / clathrin-coated endocytic vesicle membrane / ER to Golgi transport vesicle membrane / recycling endosome / phagocytic vesicle membrane / recycling endosome membrane / late endosome membrane / Cargo recognition for clathrin-mediated endocytosis / protein transport / presynapse / Clathrin-mediated endocytosis / ER-Phagosome pathway / early endosome membrane / protein phosphatase binding / defense response to virus / vesicle / membrane fusion / early endosome / Golgi membrane / lysosomal membrane / centrosome / Neutrophil degranulation / symbiont entry into host cell / endoplasmic reticulum membrane / protein kinase binding / perinuclear region of cytoplasm / mitochondrion / extracellular exosome / membrane / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Syntaxin-17 / Synaptobrevin/Vesicle-associated membrane protein / Synaptobrevin signature. / Synaptobrevin-like / Synaptobrevin / v-SNARE, coiled-coil homology domain / v-SNARE coiled-coil homology domain profile. / Syntaxin / Syntaxin/epimorphin, conserved site / Syntaxin / epimorphin family signature. ...Syntaxin-17 / Synaptobrevin/Vesicle-associated membrane protein / Synaptobrevin signature. / Synaptobrevin-like / Synaptobrevin / v-SNARE, coiled-coil homology domain / v-SNARE coiled-coil homology domain profile. / Syntaxin / Syntaxin/epimorphin, conserved site / Syntaxin / epimorphin family signature. / SNARE / Helical region found in SNAREs / t-SNARE coiled-coil homology domain profile. / Target SNARE coiled-coil homology domain
Similarity search - Domain/homology
Synaptosomal-associated protein 29 / Syntaxin-17 / Vesicle-associated membrane protein 8
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.05 Å
AuthorsLi, Y. / Pan, L.F.
Funding support China, 2items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)31470749 China
National Natural Science Foundation of China (NSFC)21621002 China
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2020
Title: Decoding three distinct states of the Syntaxin17 SNARE motif in mediating autophagosome-lysosome fusion.
Authors: Li, Y. / Cheng, X. / Li, M. / Wang, Y. / Fu, T. / Zhou, Z. / Wang, Y. / Gong, X. / Xu, X. / Liu, J. / Pan, L.
History
DepositionApr 9, 2020Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Sep 2, 2020Provider: repository / Type: Initial release
Revision 1.1Sep 16, 2020Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Nov 29, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Vesicle-associated membrane protein 8
B: Syntaxin-17
C: Synaptosomal-associated protein 29
D: Synaptosomal-associated protein 29
E: Vesicle-associated membrane protein 8
F: Syntaxin-17
G: Synaptosomal-associated protein 29
H: Synaptosomal-associated protein 29
I: Vesicle-associated membrane protein 8
J: Syntaxin-17
K: Synaptosomal-associated protein 29
L: Synaptosomal-associated protein 29
M: Vesicle-associated membrane protein 8
N: Syntaxin-17
O: Synaptosomal-associated protein 29
P: Synaptosomal-associated protein 29
Q: Vesicle-associated membrane protein 8
R: Syntaxin-17
S: Synaptosomal-associated protein 29
T: Synaptosomal-associated protein 29
U: Vesicle-associated membrane protein 8
V: Syntaxin-17
W: Synaptosomal-associated protein 29
X: Synaptosomal-associated protein 29


Theoretical massNumber of molelcules
Total (without water)214,54324
Polymers214,54324
Non-polymers00
Water00
1
A: Vesicle-associated membrane protein 8
B: Syntaxin-17
C: Synaptosomal-associated protein 29
D: Synaptosomal-associated protein 29


Theoretical massNumber of molelcules
Total (without water)35,7574
Polymers35,7574
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11830 Å2
ΔGint-98 kcal/mol
Surface area14000 Å2
MethodPISA
2
E: Vesicle-associated membrane protein 8
F: Syntaxin-17
G: Synaptosomal-associated protein 29
H: Synaptosomal-associated protein 29


Theoretical massNumber of molelcules
Total (without water)35,7574
Polymers35,7574
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11710 Å2
ΔGint-102 kcal/mol
Surface area14310 Å2
MethodPISA
3
I: Vesicle-associated membrane protein 8
J: Syntaxin-17
K: Synaptosomal-associated protein 29
L: Synaptosomal-associated protein 29


Theoretical massNumber of molelcules
Total (without water)35,7574
Polymers35,7574
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11320 Å2
ΔGint-99 kcal/mol
Surface area14310 Å2
MethodPISA
4
M: Vesicle-associated membrane protein 8
N: Syntaxin-17
O: Synaptosomal-associated protein 29
P: Synaptosomal-associated protein 29


Theoretical massNumber of molelcules
Total (without water)35,7574
Polymers35,7574
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11440 Å2
ΔGint-102 kcal/mol
Surface area14470 Å2
MethodPISA
5
Q: Vesicle-associated membrane protein 8
R: Syntaxin-17
S: Synaptosomal-associated protein 29
T: Synaptosomal-associated protein 29


Theoretical massNumber of molelcules
Total (without water)35,7574
Polymers35,7574
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11310 Å2
ΔGint-85 kcal/mol
Surface area13780 Å2
MethodPISA
6
U: Vesicle-associated membrane protein 8
V: Syntaxin-17
W: Synaptosomal-associated protein 29
X: Synaptosomal-associated protein 29


Theoretical massNumber of molelcules
Total (without water)35,7574
Polymers35,7574
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area11610 Å2
ΔGint-104 kcal/mol
Surface area14070 Å2
MethodPISA
Unit cell
Length a, b, c (Å)143.981, 196.000, 76.142
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Space group name HallP22ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x+1/2,y+1/2,-z
#4: -x,-y,z

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Components

#1: Protein
Vesicle-associated membrane protein 8 / VAMP-8 / Endobrevin / EDB


Mass: 7980.967 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: VAMP8 / Production host: Escherichia coli (E. coli) / References: UniProt: Q9BV40
#2: Protein
Syntaxin-17


Mass: 9494.307 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: STX17 / Production host: Escherichia coli (E. coli) / References: UniProt: P56962
#3: Protein
Synaptosomal-associated protein 29 / SNAP-29 / Soluble 29 kDa NSF attachment protein / Vesicle-membrane fusion protein SNAP-29


Mass: 10379.804 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SNAP29 / Production host: Escherichia coli (E. coli) / References: UniProt: O95721
#4: Protein
Synaptosomal-associated protein 29 / SNAP-29 / Soluble 29 kDa NSF attachment protein / Vesicle-membrane fusion protein SNAP-29


Mass: 7902.030 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SNAP29 / Production host: Escherichia coli (E. coli) / References: UniProt: O95721

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.5 Å3/Da / Density % sol: 50.88 %
Crystal growTemperature: 289 K / Method: vapor diffusion, sitting drop
Details: 2.5 M Ammonium nitrate, 0.1 M Sodium acetate trihydrate pH 4.6

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97776 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 4, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97776 Å / Relative weight: 1
ReflectionResolution: 3.05→50 Å / Num. obs: 41797 / % possible obs: 99.9 % / Redundancy: 6.6 % / Biso Wilson estimate: 55.56 Å2 / CC1/2: 0.99 / Net I/σ(I): 6.3
Reflection shellResolution: 3.05→3.1 Å / CC1/2: 0.93

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1GL2

1gl2


Resolution: 3.05→49 Å / SU ML: 0.4024 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 26.2647
RfactorNum. reflection% reflection
Rfree0.2554 2085 4.99 %
Rwork0.1751 --
obs0.1792 41796 99.75 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso mean: 62.3 Å2
Refinement stepCycle: LAST / Resolution: 3.05→49 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12345 0 0 0 12345
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00912427
X-RAY DIFFRACTIONf_angle_d0.998616705
X-RAY DIFFRACTIONf_chiral_restr0.05011976
X-RAY DIFFRACTIONf_plane_restr0.00452172
X-RAY DIFFRACTIONf_dihedral_angle_d25.07651668
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.05-3.120.35561480.24462596X-RAY DIFFRACTION99.89
3.12-3.20.33851450.23472604X-RAY DIFFRACTION99.82
3.2-3.290.29581390.23422591X-RAY DIFFRACTION99.93
3.29-3.380.30341420.1972608X-RAY DIFFRACTION99.85
3.38-3.490.28731190.18322640X-RAY DIFFRACTION99.93
3.49-3.620.24411400.17912640X-RAY DIFFRACTION99.96
3.62-3.760.26911480.18382600X-RAY DIFFRACTION99.89
3.76-3.930.25751420.17682614X-RAY DIFFRACTION99.93
3.93-4.140.22921430.15952624X-RAY DIFFRACTION99.64
4.14-4.40.24471250.13542660X-RAY DIFFRACTION100
4.4-4.740.21221430.14392658X-RAY DIFFRACTION100
4.74-5.210.24451370.15662665X-RAY DIFFRACTION99.79
5.21-5.970.34951410.23082682X-RAY DIFFRACTION100
5.97-7.510.28361490.1942712X-RAY DIFFRACTION99.86
7.51-490.16731240.14232817X-RAY DIFFRACTION97.94
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.187864358840.3689579298170.1745876036152.62993712862-0.0443297154280.673070257345-0.0152913768593-0.121224396851-0.0823783821138-0.3335729335690.0458143391692-0.7092658832140.0800728185987-0.0130715564021-0.05245547934890.256578155205-0.00312263838760.03339809471940.37881107882-0.0004264068337960.153938597521-16.29873260590.266045366901-27.9034783545
20.5516115976220.243689989497-0.5010952042673.14244939462-1.659188496470.875596133857-0.0556704630037-0.195814150937-0.00086712424445-0.807076167684-0.02957584391480.750619343360.0910298899201-0.0326638731920.02204303025610.236734041068-0.0542249497745-0.01670485640860.3554929940290.05293424884880.272347224049-19.98736977834.68761290296-31.6425067552
30.723212851145-0.8001750256280.1316587367143.06522561302-0.273669976230.254231923088-0.274973080363-0.0339207024645-0.0924897820721-0.4025422016010.4118383653530.150733007920.102669640863-0.175134904114-0.03814159873520.3392349290150.05433746697620.03635158984680.343509976551-0.03151402755270.272277996043-24.323388954912.1214615621-28.5383745394
40.930955835198-1.09858544041-0.1469423602128.99907408933-0.6999183431110.396468202976-0.145422283274-0.06333503853960.0582439490205-0.02827046500110.291809665539-0.135492139307-0.006345446793620.0363357253743-0.09438464128760.3530519894110.01262210705680.04834281428560.302608517027-0.002488728706510.270183386278-21.075692158312.5382396868-23.8801230851
51.725391315351.923368579710.5223283200388.463511885650.6767332639720.760490846425-0.146562855737-0.05271744838480.342973934846-0.4647748138080.07697501330811.09765018128-0.136651091746-0.2000672718540.06341649367270.2587802087190.06117612762450.03103869797330.412227553571-0.03502870513970.0728625313328-46.505184157519.439185927-64.4928948829
62.137859333032.055467818841.086045073582.771959898191.612885715621.22047053555-0.4779572609120.289423088212-0.0490158550923-0.6714879842710.709578548626-0.385213205841-0.304943078980.0137436888316-0.2347377494130.345564243781-0.0241483639920.01322197336010.3439033805350.002777587456070.208141283765-43.470493154213.8417980477-68.4913441
70.802736382060.8193782192630.2827174053178.958763419622.944364166651.77982036928-0.18592824467-0.00662563598883-0.146430359253-0.3027945105780.344495821884-0.0766592451969-0.1813628307290.186395520418-0.09591161120020.2923186756190.0940609887542-0.1031490715180.3263867698780.07065770733080.221840339159-40.78871029363.73600459427-66.712452122
81.09087481053-0.2529969493230.005320691516327.890559047552.92279820461.4638998912-0.0718722634502-0.1149994674430.05666214143350.4368686161590.2378341992850.0908531132180.2228931452140.0701431274519-0.1638494245250.2948417070420.0335125357033-0.01812214665890.4380048435670.01387610493570.217386190854-43.34068855374.75475984505-62.4857901654
95.559673232954.99336790663-2.079862782756.87262028539-2.316110683631.88357136873-0.1054978169790.5994672132790.157909180563-0.0645489599160.3844959994720.0259199895868-0.0700584910068-0.127404504273-0.2025127678930.2223539961650.07893460124530.01844844500890.428481837067-0.04374819804870.240459401939-53.273198777711.0277478271-89.1789802489
100.1036273986070.2528432400040.2341836005221.435142651731.086442274480.8394526608820.896813963056-0.06873705121460.0570556225711.71671065868-0.0677675248085-0.2034926657-0.4063556865690.575847231702-0.5287505504271.66138428746-0.6150074789150.1506054955880.877732723420.03953417919691.61922941511-28.887010705153.5437701964-89.5503070302
111.875660657773.11081623008-0.8488572997468.62397737485-1.099765602081.422149499140.575184047526-0.0738104088916-0.1303546428140.961766544162-0.491352289661-0.651663973787-0.493048855750.0545968245223-0.08197054621180.3491721291990.02152117288150.03692153809480.31285117009-0.03738506500860.324036333345-48.98077031214.8265862369-83.2310648376
120.04476173208670.0006925625379380.02521498288560.0363680555912-0.001267684667850.0103733102369-0.527481396640.694377234565-0.00842841086672-0.8685551209160.223791424138-0.2903496292110.4400424122451.47329693470.1903900832681.041824980270.102455940631-0.09912910284731.42033440137-0.5239903704481.07123336324-67.4257462716-34.3222226174-69.759736343
132.48434085542.65858548964-1.507151999178.97621244739-3.00270165461.915256344890.2575653710880.1283493576010.3978333241170.2110570376360.0194068020730.38498799823-0.2764036616820.00250623679603-0.2619688795440.2812004274810.08589385235330.04547742608510.4267201329590.06054239072820.286216118614-54.590488513918.4113499418-81.2898616843
143.842133319724.29321413029-1.649700818129.68024420077-3.714260356182.37674033658-0.213089895370.5569383809990.226381028140.01164059901820.5688442401660.308305372052-0.0649053937204-0.460409380909-0.2793433325870.2228998432980.08441726847320.03670948845780.393711103364-0.03125467530910.228873464858-58.811706051212.4203420312-84.4676995566
154.432942524192.340558958031.332493648388.045783127091.021659207110.8402831286090.0474441851056-0.375609419093-0.4802432968960.3515773549190.10487356868-1.34168407971-0.058936445990.144069819444-0.0224546593880.3365139454620.0173688936783-0.006181006079280.437422740581-0.07913857828430.125845972746-30.34415379975.28968448686-79.729122042
164.18524585847-4.7018942660.8706263134715.30747882649-1.173065186873.311224137040.3008034927910.463033151629-0.178823475813-0.676351358271-0.117556829155-0.815148442519-0.194786559428-1.39210202817-0.09721208988280.8475986364160.0663528451431-0.2771543599350.8378934473330.08577430368522.10855715308-11.068820960548.6193150046-71.7060445105
174.818099943025.209138554810.5000862415728.05268748655-0.05660352747430.9126243345170.287179453717-0.02963393369990.698457904860.7273407221-0.2728965566970.45799212612-0.0724920664352-0.00476233261181-0.1106236394930.2425617052460.0642627176369-0.04758526238490.276931913888-0.03283687187840.411159980168-34.616455810410.1039723371-81.1809048403
184.299038142232.574576987881.491109500575.09602433291.434545590621.532255786450.02561285667250.5942900032450.4047159429680.04468445389660.244683703470.4166452607230.00473370966770.0765593133139-0.2210934092310.2380210046770.02684590372980.06597972521470.437095297598-0.02902987756030.120003485977-42.76582696120.397370464702-89.7668046514
190.200558872701-0.5678786375-1.053837599931.991550842752.251214467486.550997206860.90052694148-0.4081357305390.0196672408637-0.8617183169750.152371880613-0.601039965483-1.21032329258-0.217066998771-0.7007688656030.5862675965280.09622043217750.2361369015610.878336233829-0.2630953896572.19073982584-4.4003464220236.0728086415-81.3426127149
203.563904906110.27954898237-0.07542816519943.301332633830.4745251433810.84264898950.210814036240.350904293024-0.3978202087650.5035066709510.224120177888-0.6586947898820.2451734895120.155633103176-0.3071010714520.302612400972-0.0108416623790.02032749730810.2838120340610.04046940064550.149719278612-32.31732487013.01963582669-87.9416073177
214.357716162532.87483111699-0.1446517192018.550517445632.4034217272.78133352373-0.4973174980381.079722180450.0479064713369-0.6370046637980.5711791709150.559412204421-0.00159434012810.0604246024465-0.03604223349580.3048815679910.0637268795597-0.03594300240670.5754705052440.04649258219480.21423300935-9.9327825319811.4291944941-50.7832322902
220.543605302330.6457399379310.6211421306670.7597810471450.7321447798170.695827299056-0.608103911143-0.5690616736240.6263128512920.418272899198-0.8034195844030.3645311728370.111741556191-0.1732376429540.8340048115291.34150979498-0.0306097522364-0.2888565585160.908312825729-0.3003129091342.29046925803-30.0821515464-28.4470750216-59.9039538129
233.049971205141.461476150430.9044844308549.505941020632.244056397511.635820158870.1612915613990.236206580022-0.1032494672450.772189559865-0.131440840519-0.09778510086730.341776067814-0.3048209141020.03214870703650.414530644193-0.04954286424360.06988786189080.4032126499160.05273349549620.157744277596-12.550834058814.2082048265-43.7553676453
241.245875131470.03296523103221.60376425917-0.0007231599060720.03412975911972.08816049592-0.0127767723884-0.650590572618-0.379784868087-0.6932999093910.691598172609-0.8252397366071.604165382661.12812317582-0.2907032664371.15217166047-0.2893844610970.5214528365381.13470691585-0.7345391284231.40703048186-20.8896348238-24.8270609667-53.0808417308
252.317555523631.255543494240.332185436788.895736422693.805601833683.36113537075-0.1300194709040.255622475353-0.05137605127980.480918539851-0.2090388086340.3375170896480.467049130726-0.2177606506140.1189881738090.2678587187090.02821794939610.03362842601840.3997416268580.03580662951280.258819841726-7.0442374425110.249327397-41.1363306339
262.770778782971.561734921830.4512114790624.262014747271.536164898152.10451267047-0.1860406631750.6197368249760.0930635591483-0.07096866434960.245932795446-0.1760216386310.3719765323990.318281170021-0.1062089954410.3980178275230.0235016212087-0.0125014898090.4039623931150.04586127283540.198925569543-4.1896930360110.0946815091-45.3653050553
273.29111756333.19221739328-0.6053464354088.72190064889-0.9635893267831.015315798640.162115787861-0.0452457236718-0.09547820940380.650981581813-0.1094515929850.570396645072-0.0604982891163-0.0963434957666-0.1309523101830.2395933940610.0619703748062-0.06163502027310.3812046869130.09666177234470.291205094472-36.06449981087.86554210591-42.032914473
284.549265476563.95619919990.4891286221947.35761395638-0.1450874361110.519195239556-0.1727841267640.532349257731-0.1630756040310.01307580091560.4643364208470.1297729462610.04936510905290.00215941850566-0.3388680034410.3673533402950.05226363729360.01715857244930.3683137794010.03914619370830.24425621044-29.51350955759.62532009803-43.9380463137
296.847322242416.92091703667-0.6702733942729.4422108664-0.8091504643040.994390325418-0.1062503695440.365905760604-0.415550035785-0.109132043410.153532331243-0.573102592978-0.07279346375160.106637456951-0.05708993413850.2826361417030.0140196736558-0.01770948448490.4205199453620.08126712594340.276528479796-22.418731554520.108594471-50.5224636688
301.235193827780.9337287226831.308346998490.6956851758251.033516508166.155257995170.105918860893-0.411726541462-0.439947035731-0.455150533228-0.1093928189920.3773786717480.802457547463-1.395997393440.01748638700290.642444126152-0.0531783970182-0.3167279662310.5481234374030.1680554884681.75016444252-58.0128455324-17.9340240532-47.2786208829
310.1754752135-0.1400820355530.3466243864265.20310449791-2.401740289223.35821078961-0.2050969573230.3725050159140.188850392943-0.76933375168-0.151483221146-0.6435764107620.1399507085170.5203141513580.07107336260181.577578899950.005629373484640.2466160942871.084411805530.407334936930.7992879055371.2786919432944.5110475399-57.9871827738
325.610553971132.93641148411-0.07077620903365.15599761693-0.980985804280.798633557748-0.1158513619370.9784469495640.6314861211430.1982371986750.4902558497630.915908883675-0.0300538951188-0.253380043824-0.3325534723220.3795658672580.0155851741470.07150069420240.392118493691-0.03364240924070.177118558406-34.986216841314.8611737734-48.5499996445
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 9 through 74 )
2X-RAY DIFFRACTION2chain 'B' and (resid 168 through 228 )
3X-RAY DIFFRACTION3chain 'C' and (resid 43 through 114 )
4X-RAY DIFFRACTION4chain 'D' and (resid 192 through 258 )
5X-RAY DIFFRACTION5chain 'E' and (resid 10 through 74 )
6X-RAY DIFFRACTION6chain 'F' and (resid 168 through 228 )
7X-RAY DIFFRACTION7chain 'G' and (resid 41 through 114 )
8X-RAY DIFFRACTION8chain 'H' and (resid 191 through 258 )
9X-RAY DIFFRACTION9chain 'I' and (resid 9 through 60 )
10X-RAY DIFFRACTION10chain 'I' and (resid 61 through 74 )
11X-RAY DIFFRACTION11chain 'J' and (resid 168 through 226 )
12X-RAY DIFFRACTION12chain 'K' and (resid 42 through 50 )
13X-RAY DIFFRACTION13chain 'K' and (resid 51 through 114 )
14X-RAY DIFFRACTION14chain 'L' and (resid 192 through 258 )
15X-RAY DIFFRACTION15chain 'M' and (resid 9 through 66 )
16X-RAY DIFFRACTION16chain 'M' and (resid 67 through 74 )
17X-RAY DIFFRACTION17chain 'N' and (resid 168 through 227 )
18X-RAY DIFFRACTION18chain 'O' and (resid 42 through 104 )
19X-RAY DIFFRACTION19chain 'O' and (resid 105 through 114 )
20X-RAY DIFFRACTION20chain 'P' and (resid 194 through 258 )
21X-RAY DIFFRACTION21chain 'Q' and (resid 9 through 59 )
22X-RAY DIFFRACTION22chain 'Q' and (resid 60 through 69 )
23X-RAY DIFFRACTION23chain 'R' and (resid 168 through 213 )
24X-RAY DIFFRACTION24chain 'R' and (resid 214 through 222 )
25X-RAY DIFFRACTION25chain 'S' and (resid 41 through 114 )
26X-RAY DIFFRACTION26chain 'T' and (resid 191 through 257 )
27X-RAY DIFFRACTION27chain 'U' and (resid 9 through 74 )
28X-RAY DIFFRACTION28chain 'V' and (resid 168 through 227 )
29X-RAY DIFFRACTION29chain 'W' and (resid 43 through 104 )
30X-RAY DIFFRACTION30chain 'W' and (resid 105 through 114 )
31X-RAY DIFFRACTION31chain 'X' and (resid 194 through 198 )
32X-RAY DIFFRACTION32chain 'X' and (resid 199 through 258 )

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