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- PDB-7bmv: p62PH in cesium chloride (0.25 M CsCl in protein buffer and cryo ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7bmv | ||||||
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Title | p62PH in cesium chloride (0.25 M CsCl in protein buffer and cryo protectant) | ||||||
![]() | RNA polymerase II transcription factor B 73 kDa subunit-like protein | ||||||
![]() | TRANSCRIPTION / Cesium / Phasing / Pleckstrin homology domain | ||||||
Function / homology | ![]() transcription factor TFIIH core complex / transcription factor TFIIH holo complex / transcription by RNA polymerase I / nucleotide-excision repair / transcription by RNA polymerase II Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Koelmel, W. / Kuper, J. / Kisker, C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Cesium based phasing of macromolecules: a general easy to use approach for solving the phase problem. Authors: Koelmel, W. / Kuper, J. / Kisker, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.6 KB | Display | ![]() |
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PDB format | ![]() | 24.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 980.7 KB | Display | ![]() |
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Full document | ![]() | 980.7 KB | Display | |
Data in XML | ![]() | 7.2 KB | Display | |
Data in CIF | ![]() | 9.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7bmoC ![]() 7bmpC ![]() 7bmqC ![]() 7bmrC ![]() 7bmsC ![]() 7bmtC ![]() 7bmuC ![]() 7bmwC ![]() 7bmxC ![]() 7bmyC ![]() 7bmzC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 15184.287 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: DSM 1495 / CBS 144.50 / IMI 039719 / Gene: CTHT_0001260 / Production host: ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | ChemComp-CL / | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.06 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / Details: 0.6 M potassium chloride, 15 % (w/v) PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 30, 2016 | ||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1.7712 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 1.9→42.27 Å / Num. obs: 12135 / % possible obs: 97.8 % / Redundancy: 23.7 % / CC1/2: 0.999 / Rmerge(I) obs: 0.093 / Rpim(I) all: 0.019 / Rrim(I) all: 0.095 / Net I/σ(I): 21.6 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 117.18 Å2 / Biso mean: 43.584 Å2 / Biso min: 24.07 Å2
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Refinement step | Cycle: final / Resolution: 1.9→42.27 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.949 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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