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Structure paper

TitleCesium based phasing of macromolecules: a general easy to use approach for solving the phase problem.
Journal, issue, pagesSci Rep, Vol. 11, Page 17038-17038, Year 2021
Publish dateJan 20, 2021 (structure data deposition date)
AuthorsKoelmel, W. / Kuper, J. / Kisker, C.
External linksSci Rep / PubMed:34426585
MethodsX-ray diffraction
Resolution1.47 - 2.5 Å
Structure data

PDB-7bmo:
HEWL in cesium chloride (0.25 M CsCl in protein buffer)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7bmp:
HEWL in cesium chloride (0.25 M CsCl in protein buffer and cryo protectant).
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7bmq:
HEWL in cesium chloride (1.71 M CsCl in cryo protectant)
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

PDB-7bmr:
HEWL in cesium chloride (0.25 M CsCl in protein buffer and 1.71 M CsCl in cryo protectant)
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-7bms:
HEWL in cesium chloride (1.5 M CsCl in crystallization condition and cryo protectant)
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-7bmt:
HEWL in sodium chloride
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-7bmu:
p62PH in cesium chloride (0.25 M CsCl in protein buffer)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7bmv:
p62PH in cesium chloride (0.25 M CsCl in protein buffer and cryo protectant)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7bmw:
p62PH in cesium chloride (0.75 M CsCl in cryo protectant)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7bmx:
p62PH in cesium chloride (0.25 M CsCl in protein buffer and 0.75 M CsCl in cryo protectant)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7bmy:
p62PH in cesium chloride (0.7 M CsCl in crystallization condition and cryo protectant)
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-7bmz:
p62PH in potassium chloride
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-CS:
Unknown entry

ChemComp-K:
Unknown entry

Source
  • gallus gallus (chicken)
  • chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (fungus)
KeywordsHYDROLASE / Cesium / Phasing / TRANSCRIPTION / Pleckstrin homology domain

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