- PDB-7bgf: CRYSTAL STRUCTURE OF THE N-TERMINAL DIMERIC COILED COIL OF THE HU... -
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Entry
Database: PDB / ID: 7bgf
Title
CRYSTAL STRUCTURE OF THE N-TERMINAL DIMERIC COILED COIL OF THE HUMAN CTIP PROTEIN
Components
DNA endonuclease RBBP8,CtIP/RBBP8
Keywords
DNA BINDING PROTEIN / COILED COIL / DIMER / DNA REPAIR / HOMOLOGOUS RECOMBINATION
Function / homology
Function and homology information
DNA double-strand break processing involved in repair via single-strand annealing / BRCA1-C complex / blastocyst hatching / DNA strand resection involved in replication fork processing / single-stranded DNA endodeoxyribonuclease activity / homologous recombination / Impaired BRCA2 binding to PALB2 / HDR through MMEJ (alt-NHEJ) / mitotic G2/M transition checkpoint / Defective homologous recombination repair (HRR) due to BRCA1 loss of function ...DNA double-strand break processing involved in repair via single-strand annealing / BRCA1-C complex / blastocyst hatching / DNA strand resection involved in replication fork processing / single-stranded DNA endodeoxyribonuclease activity / homologous recombination / Impaired BRCA2 binding to PALB2 / HDR through MMEJ (alt-NHEJ) / mitotic G2/M transition checkpoint / Defective homologous recombination repair (HRR) due to BRCA1 loss of function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA1 binding function / Defective HDR through Homologous Recombination Repair (HRR) due to PALB2 loss of BRCA2/RAD51/RAD51C binding function / Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Synthesis-Dependent Strand Annealing (SDSA) / Resolution of D-loop Structures through Holliday Junction Intermediates / HDR through Single Strand Annealing (SSA) / Impaired BRCA2 binding to RAD51 / Transcriptional Regulation by E2F6 / Presynaptic phase of homologous DNA pairing and strand exchange / transcription repressor complex / meiotic cell cycle / double-strand break repair via homologous recombination / HDR through Homologous Recombination (HRR) / G2/M DNA damage checkpoint / Meiotic recombination / G1/S transition of mitotic cell cycle / transcription corepressor activity / site of double-strand break / Processing of DNA double-strand break ends / RNA polymerase II-specific DNA-binding transcription factor binding / Regulation of TP53 Activity through Phosphorylation / damaged DNA binding / Hydrolases; Acting on ester bonds / cell division / intracellular membrane-bounded organelle / DNA repair / regulation of transcription by RNA polymerase II / nucleoplasm / identical protein binding / nucleus / metal ion binding Similarity search - Function
DNA endonuclease Ctp1, N-terminal / DNA endonuclease RBBP8-like / Tumour-suppressor protein CtIP N-terminal domain / DNA endonuclease activator Ctp1, C-terminal / DNA endonuclease activator SAE2/CtIP C-terminus Similarity search - Domain/homology
DNAendonucleaseRBBP8,CtIP/RBBP8 / CtBP-interacting protein / CtIP / Retinoblastoma-binding protein 8 / RBBP-8 / Retinoblastoma- ...CtBP-interacting protein / CtIP / Retinoblastoma-binding protein 8 / RBBP-8 / Retinoblastoma-interacting protein and myosin-like / RIM / Sporulation in the absence of SPO11 protein 2 homolog / SAE2
Mass: 15273.274 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The construct expresses amino acids 31 to 145 of human CtIP. It contains two single-point mutations: C89 and C92 have been mutated to alanine. The GSMG peptide at the N-terminus contains the ...Details: The construct expresses amino acids 31 to 145 of human CtIP. It contains two single-point mutations: C89 and C92 have been mutated to alanine. The GSMG peptide at the N-terminus contains the amino acids left over after TEV cleavage of the MBP tag, and is not part of the CtIP sequence. The WSHPQFEK sequence at the C-terminus represents the Strep tag peptide and is not part of the CtIP sequence.,The construct expresses amino acids 31 to 145 of human CtIP. It contains two single-point mutations: C89 and C92 have been mutated to alanine. The GSMG peptide at the N-terminus contains the amino acids left over after TEV cleavage of the MBP tag, and is not part of the CtIP sequence. The WSHPQFEK sequence at the C-terminus represents the Strep tag peptide and is not part of the CtIP sequence. Source: (gene. exp.) Homo sapiens (human) / Gene: RBBP8, CTIP / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta2 References: UniProt: Q99708, Hydrolases; Acting on ester bonds
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