GUANOSINE-5'-TRIPHOSPHATE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A ...GUANOSINE-5'-TRIPHOSPHATE / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Small ribosomal subunit protein uS4A / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein uS11A / Small ribosomal subunit protein uS8A / Small ribosomal subunit protein uS12A / Small ribosomal subunit protein eS24A / Small ribosomal subunit protein eS4A / Small ribosomal subunit protein eS6A / Small ribosomal subunit protein eS8A / Small ribosomal subunit protein uS17A / Small ribosomal subunit protein uS9A / Small ribosomal subunit protein uS13A / rRNA 2'-O-methyltransferase fibrillarin / Exosome complex component RRP43 / Ribosomal RNA-processing protein 7 / KRR1 small subunit processome component / Periodic tryptophan protein 2 / Small ribosomal subunit protein uS7 / Small ribosomal subunit protein eS7A / U3 small nucleolar ribonucleoprotein protein IMP3 / Small ribosomal subunit protein eS1A / Protein SOF1 / U3 small nucleolar RNA-associated protein 11 / U3 small nucleolar RNA-associated protein 20 / Small ribosomal subunit protein eS27A / Ribosome biogenesis protein UTP30 / Essential nuclear protein 1 / Exosome complex component RRP4 / U3 small nucleolar RNA-associated protein 9 / 13 kDa ribonucleoprotein-associated protein / U3 small nucleolar RNA-associated protein 7 / U3 small nucleolar RNA-associated protein 18 / Regulator of rDNA transcription protein 14 / Protein FAF1 / U3 small nucleolar RNA-associated protein 10 / Exosome complex component SKI6 / ATP-dependent RNA helicase DOB1 / U3 small nucleolar RNA-associated protein MPP10 / Ribosome biogenesis protein ENP2 / Exosome complex component MTR3 / U3 small nucleolar RNA-associated protein 22 / Exosome complex component RRP46 / U3 small nucleolar RNA-associated protein 8 / Exosome complex component CSL4 / RNA cytidine acetyltransferase / U3 small nucleolar ribonucleoprotein protein IMP4 / U3 small nucleolar RNA-associated protein 6 / NET1-associated nuclear protein 1 / U3 small nucleolar RNA-associated protein 5 / U3 small nucleolar RNA-associated protein 15 / U3 small nucleolar RNA-associated protein 14 / rRNA biogenesis protein RRP5 / rRNA-processing protein FCF1 / Exosome complex component RRP45 / U3 small nucleolar RNA-associated protein 13 / U3 small nucleolar RNA-associated protein 21 / Ribosomal RNA small subunit methyltransferase NEP1 / Ribosomal RNA-processing protein 9 / Nucleolar complex protein 4 / Protein BFR2 / U3 small nucleolar RNA-associated protein 4 / RNA 3'-terminal phosphate cyclase-like protein / Exosome complex exonuclease DIS3 / Exosome complex component RRP40 / U3 small nucleolar RNA-associated protein 16 / Ribosome biogenesis protein BMS1 / rRNA-processing protein FCF2 / Something about silencing protein 10 / U3 small nucleolar RNA-associated protein 12 / Exosome complex component RRP42 / Nucleolar protein 56 / Nucleolar protein 58 / Small ribosomal subunit protein eS28A / Nucleolar complex protein 14 / Pre-rRNA-processing protein PNO1 類似検索 - 構成要素
ジャーナル: Mol Cell / 年: 2021 タイトル: Structure of the Maturing 90S Pre-ribosome in Association with the RNA Exosome. 著者: Benjamin Lau / Jingdong Cheng / Dirk Flemming / Giuseppe La Venuta / Otto Berninghausen / Roland Beckmann / Ed Hurt / 要旨: Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in ...Ribosome assembly is catalyzed by numerous trans-acting factors and coupled with irreversible pre-rRNA processing, driving the pathway toward mature ribosomal subunits. One decisive step early in this progression is removal of the 5' external transcribed spacer (5'-ETS), an RNA extension at the 18S rRNA that is integrated into the huge 90S pre-ribosome structure. Upon endo-nucleolytic cleavage at an internal site, A, the 5'-ETS is separated from the 18S rRNA and degraded. Here we present biochemical and cryo-electron microscopy analyses that depict the RNA exosome, a major 3'-5' exoribonuclease complex, in a super-complex with the 90S pre-ribosome. The exosome is docked to the 90S through its co-factor Mtr4 helicase, a processive RNA duplex-dismantling helicase, which strategically positions the exosome at the base of 5'-ETS helices H9-H9', which are dislodged in our 90S-exosome structures. These findings suggest a direct role of the exosome in structural remodeling of the 90S pre-ribosome to drive eukaryotic ribosome synthesis.
CA: rRNA 2'-O-methyltransferase fibrillarin DA: 40S ribosomal protein S1-A JA: RNA cytidine acetyltransferase UA: Periodic tryptophan protein 2 CB: rRNA 2'-O-methyltransferase fibrillarin JB: RNA cytidine acetyltransferase UB: Nucleolar complex protein 14 UC: Something about silencing protein 10 UD: U3 small nucleolar RNA-associated protein 4 UE: U3 small nucleolar RNA-associated protein 5 UF: U3 small nucleolar RNA-associated protein 6 UG: U3 small nucleolar RNA-associated protein 7 UH: U3 small nucleolar RNA-associated protein 8 UI: U3 small nucleolar RNA-associated protein 9 UJ: U3 small nucleolar RNA-associated protein 10 UK: U3 small nucleolar RNA-associated protein 11 UL: U3 small nucleolar RNA-associated protein 12 UM: U3 small nucleolar RNA-associated protein 13 UN: U3 small nucleolar RNA-associated protein 14 UO: U3 small nucleolar RNA-associated protein 15 UP: Bud site selection protein 21 UQ: NET1-associated nuclear protein 1 UR: U3 small nucleolar RNA-associated protein 18 US: Nucleolar complex protein 4 UT: U3 small nucleolar RNA-associated protein 20 UU: U3 small nucleolar RNA-associated protein 21 UV: U3 small nucleolar RNA-associated protein 22 UX: rRNA-processing protein FCF1 UZ: Ribosome biogenesis protein UTP30 CD: Nucleolar protein 56 CE: Nucleolar protein 58 CF: 13 kDa ribonucleoprotein-associated protein CG: 13 kDa ribonucleoprotein-associated protein CH: Ribosomal RNA-processing protein 9 CI: U3 small nucleolar ribonucleoprotein protein IMP3 CJ: U3 small nucleolar ribonucleoprotein protein IMP4 CK: U3 small nucleolar RNA-associated protein MPP10 CL: Ribosome biogenesis protein BMS1 CM: RNA 3'-terminal phosphate cyclase-like protein CN: Ribosomal RNA-processing protein 7 JC: Ribosome biogenesis protein ENP2 JF: Ribosomal RNA small subunit methyltransferase NEP1 JG: Ribosomal RNA small subunit methyltransferase NEP1 JH: Essential nuclear protein 1 JI: rRNA biogenesis protein RRP5 JJ: Pre-rRNA-processing protein PNO1 JK: Protein BFR2 JM: rRNA-processing protein FCF2 JN: Protein FAF1 JO: KRR1 small subunit processome component JP: Protein SOF1 JQ: Regulator of rDNA transcription protein 14 DE: 40S ribosomal protein S4-A DF: 40S ribosomal protein S5 DG: 40S ribosomal protein S6-A DH: 40S ribosomal protein S7-A DI: 40S ribosomal protein S8-A DJ: 40S ribosomal protein S9-A DL: 40S ribosomal protein S11-A DN: 40S ribosomal protein S13 DO: 40S ribosomal protein S14-A DQ: 40S ribosomal protein S16-A DS: 40S ribosomal protein S18-A DW: 40S ribosomal protein S22-A DX: 40S ribosomal protein S23-A DY: 40S ribosomal protein S24-A Db: 40S ribosomal protein S27-A Dc: 40S ribosomal protein S28-A D2: 5'ETS RNA D3: 18S rRNA D4: U3 snoRNA EA: RNA EB: Exosome complex component RRP45 EC: Exosome complex component SKI6 ED: Exosome complex component RRP43 EE: Exosome complex component RRP46 EF: Exosome complex component RRP42 EG: Exosome complex component MTR3 EH: Exosome complex component RRP40 EI: Exosome complex component RRP4 EJ: Exosome complex component CSL4 EK: Exosome complex exonuclease DIS3 EN: ATP-dependent RNA helicase DOB1 ヘテロ分子
NET1-associatednuclearprotein1 / U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar RNA- ...U three protein 17 / t-17 / U3 protein 17 required for transcription / U3 small nucleolar RNA-associated protein 17 / U3 snoRNA-associated protein 17
rRNAbiogenesisproteinRRP5 / Ribosomal RNA-processing protein 5 / U3 small nucleolar RNA-associated protein RRP5 / U3 snoRNA- ...Ribosomal RNA-processing protein 5 / U3 small nucleolar RNA-associated protein RRP5 / U3 snoRNA-associated protein RRP5