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Open data
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Basic information
Entry | Database: PDB / ID: 7a8l | ||||||
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Title | rsGreen0.7-K206A-N205C in the green-on state | ||||||
![]() | Green fluorescent protein | ||||||
![]() | FLUORESCENT PROTEIN / Reversible photoswitchable fluorescent protein | ||||||
Function / homology | Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / bioluminescence / generation of precursor metabolites and energy / Green fluorescent protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | De Zitter, E. / Dedecker, P. / Van Meervelt, L. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure-Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System*. Authors: De Zitter, E. / Hugelier, S. / Duwe, S. / Vandenberg, W. / Tebo, A.G. / Van Meervelt, L. / Dedecker, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.4 KB | Display | ![]() |
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PDB format | ![]() | 50.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.7 KB | Display | ![]() |
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Full document | ![]() | 435.4 KB | Display | |
Data in XML | ![]() | 13.8 KB | Display | |
Data in CIF | ![]() | 19.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7a7kC ![]() 7a7lC ![]() 7a7mC ![]() 7a7nC ![]() 7a7oC ![]() 7a7pC ![]() 7a7qC ![]() 7a7rC ![]() 7a7sC ![]() 7a7tC ![]() 7a7uC ![]() 7a7vC ![]() 7a7wC ![]() 7a7xC ![]() 7a7yC ![]() 7a7zC ![]() 7a80C ![]() 7a81C ![]() 7a82C ![]() 7a83C ![]() 7a84C ![]() 7a85C ![]() 7a86C ![]() 7a87C ![]() 7a88C ![]() 7a89C ![]() 7a8aC ![]() 7a8bC ![]() 7a8cC ![]() 7a8dC ![]() 7a8eC ![]() 7a8fC ![]() 7a8gC ![]() 7a8hC ![]() 7a8iC ![]() 7a8jC ![]() 7a8kC ![]() 7a8mC ![]() 7a8nC ![]() 7a8oC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 30600.377 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.61 Å3/Da / Density % sol: 52.94 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / Details: 100 mM MES pH 6.5 25 % PEG 3000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS3 2M / Detector: PIXEL / Date: Feb 22, 2017 | ||||||||||||||||||||||||
Radiation | Monochromator: Si111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||||||||
Reflection | Resolution: 1.75→46.71 Å / Num. obs: 27802 / % possible obs: 100 % / Redundancy: 11.3 % / Biso Wilson estimate: 25.61 Å2 / CC1/2: 1 / Rmerge(I) obs: 0.043 / Rpim(I) all: 0.013 / Rrim(I) all: 0.045 / Net I/σ(I): 33.1 / Num. measured all: 312942 / Scaling rejects: 2 | ||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Resolution: 1.75→46.709 Å / SU ML: 0.22 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 25.94
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 103.15 Å2 / Biso mean: 32.6807 Å2 / Biso min: 12.68 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 1.75→46.709 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10 / % reflection obs: 100 %
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