+Open data
-Basic information
Entry | Database: PDB / ID: 7a1v | ||||||
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Title | Crystal structure of YTHDF2 in complex with m1A | ||||||
Components | YTH domain-containing family protein 2 | ||||||
Keywords | RNA BINDING PROTEIN / epitranscriptomic reader | ||||||
Function / homology | Function and homology information C5-methylcytidine-containing RNA reader activity / organelle assembly / spermatogonial cell division / regulation of meiotic cell cycle process involved in oocyte maturation / regulation of rRNA processing / positive regulation of cap-independent translational initiation / endothelial to hematopoietic transition / gamete generation / embryonic morphogenesis / N6-methyladenosine-containing RNA reader activity ...C5-methylcytidine-containing RNA reader activity / organelle assembly / spermatogonial cell division / regulation of meiotic cell cycle process involved in oocyte maturation / regulation of rRNA processing / positive regulation of cap-independent translational initiation / endothelial to hematopoietic transition / gamete generation / embryonic morphogenesis / N6-methyladenosine-containing RNA reader activity / negative regulation of stem cell differentiation / oocyte maturation / regulation of hematopoietic stem cell differentiation / hematopoietic stem cell proliferation / negative regulation of type I interferon-mediated signaling pathway / mRNA destabilization / negative regulation of Notch signaling pathway / mRNA catabolic process / humoral immune response / centriolar satellite / regulation of neurogenesis / regulation of cell adhesion / stress granule assembly / regulation of mRNA stability / P-body / cytoplasmic ribonucleoprotein granule / cytoplasmic stress granule / cell cycle / innate immune response / mRNA binding / RNA binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Wang, X. / Caflisch, A. | ||||||
Funding support | Switzerland, 1items
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Citation | Journal: To Be Published Title: Crystal structure of YTHDF2 in complex with m1A Authors: Wang, X. / Caflisch, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7a1v.cif.gz | 83.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7a1v.ent.gz | 56.1 KB | Display | PDB format |
PDBx/mmJSON format | 7a1v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7a1v_validation.pdf.gz | 381 KB | Display | wwPDB validaton report |
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Full document | 7a1v_full_validation.pdf.gz | 381.3 KB | Display | |
Data in XML | 7a1v_validation.xml.gz | 13.8 KB | Display | |
Data in CIF | 7a1v_validation.cif.gz | 18.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a1/7a1v ftp://data.pdbj.org/pub/pdb/validation_reports/a1/7a1v | HTTPS FTP |
-Related structure data
Related structure data | 4wqnS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS oper: (Code: givenMatrix: (-0.987130762995, 0.129825632462, 0.0933710978037), (0.125437217629, 0.99076656374, -0.0514501759867), (-0.0991885133613, -0.0390758407626, -0.994301120127)Vector: 79. ...NCS oper: (Code: given Matrix: (-0.987130762995, 0.129825632462, 0.0933710978037), Vector: |
-Components
#1: Protein | Mass: 19367.848 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: YTHDF2, HGRG8 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q9Y5A9 #2: Chemical | ChemComp-M1A / | #3: Chemical | ChemComp-SO4 / #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.69 Å3/Da / Density % sol: 54.33 % |
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Crystal grow | Temperature: 277.15 K / Method: vapor diffusion, hanging drop / pH: 5.2 Details: 0.1M Sodium Citrate Tribasic Dihydrate pH 5.2, 2 M ammonium sulfate. PH range: 4.8-5.4 / Temp details: cold room |
-Data collection
Diffraction | Mean temperature: 100 K / Ambient temp details: boiled-off liquid nitrogen / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1 Å | |||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: Feb 16, 2020 | |||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.2→43.87 Å / Num. obs: 40931 / % possible obs: 99.98 % / Redundancy: 7.1 % / Biso Wilson estimate: 49.21 Å2 / CC1/2: 0.999 / Rrim(I) all: 0.041 / Net I/σ(I): 31 | |||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4wqn Resolution: 2.2→43.87 Å / SU ML: 0.3031 / Cross valid method: FREE R-VALUE / σ(F): 1.38 / Phase error: 29.0249 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 57.29 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→43.87 Å
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Refine LS restraints |
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Refine LS restraints NCS | Type: Torsion NCS / Rms dev position: 1.35571892648 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
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