温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.25 詳細: Well solution: 0.1 M Na-Citrate pH 4.25 / 17.5% PEG 6K JM12 was added to the soaking solution at 4 mM concentration. In the soaking drop, Citrate is replaced by Acetate (0.3 M) 150 mM NaCl 0. ...詳細: Well solution: 0.1 M Na-Citrate pH 4.25 / 17.5% PEG 6K JM12 was added to the soaking solution at 4 mM concentration. In the soaking drop, Citrate is replaced by Acetate (0.3 M) 150 mM NaCl 0.1 M Bis-Tris pH 6.2 0.3 M Na-Acetate pH 5.2 25% PEG 6K PEG 6K INCREASED to 25% FOR CRYO-PROTECTION
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データ収集
回折
平均測定温度: 100 K / Serial crystal experiment: N
放射光源
由来: シンクロトロン / サイト: MAX IV / ビームライン: BioMAX / 波長: 0.9184 Å
解像度: 1.77→39.7 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.943 / SU B: 2.902 / SU ML: 0.09 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.145 / ESU R Free: 0.127 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2084
2166
4.9 %
RANDOM
Rwork
0.1786
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obs
0.1801
41977
95.37 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK