[English] 日本語
![](img/lk-miru.gif)
- PDB-6zvq: Complex between SMAD2 MH2 domain and peptide from Ski corepressor -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 6zvq | ||||||
---|---|---|---|---|---|---|---|
Title | Complex between SMAD2 MH2 domain and peptide from Ski corepressor | ||||||
![]() |
| ||||||
![]() | TRANSCRIPTION / Phosphoserine / Signal transduction / Transcription modulation | ||||||
Function / homology | ![]() nose morphogenesis / histone deacetylase inhibitor activity / zygotic specification of dorsal/ventral axis / regulation of binding / homomeric SMAD protein complex / paraxial mesoderm morphogenesis / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway ...nose morphogenesis / histone deacetylase inhibitor activity / zygotic specification of dorsal/ventral axis / regulation of binding / homomeric SMAD protein complex / paraxial mesoderm morphogenesis / activin responsive factor complex / SMAD4 MH2 Domain Mutants in Cancer / SMAD2/3 MH2 Domain Mutants in Cancer / nodal signaling pathway / SMAD protein complex / myotube differentiation / endoderm formation / co-SMAD binding / heteromeric SMAD protein complex / determination of left/right asymmetry in lateral mesoderm / odontoblast differentiation / secondary palate development / pericardium development / FOXO-mediated transcription of cell cycle genes / lens morphogenesis in camera-type eye / SMAD2/3 Phosphorylation Motif Mutants in Cancer / TGFBR1 KD Mutants in Cancer / negative regulation of Schwann cell proliferation / Transcriptional regulation of pluripotent stem cells / regulation of transforming growth factor beta receptor signaling pathway / embryonic foregut morphogenesis / primary miRNA processing / transforming growth factor beta receptor binding / Germ layer formation at gastrulation / camera-type eye morphogenesis / olfactory bulb development / pulmonary valve morphogenesis / Signaling by BMP / myelination in peripheral nervous system / type I transforming growth factor beta receptor binding / Formation of definitive endoderm / signal transduction involved in regulation of gene expression / activin receptor signaling pathway / Signaling by Activin / SMAD protein signal transduction / negative regulation of activin receptor signaling pathway / Formation of axial mesoderm / positive regulation of BMP signaling pathway / Signaling by NODAL / response to cholesterol / embryonic cranial skeleton morphogenesis / camera-type eye development / I-SMAD binding / aortic valve morphogenesis / pancreas development / bone morphogenesis / embryonic limb morphogenesis / insulin secretion / face morphogenesis / anterior/posterior axis specification / anterior/posterior pattern specification / cardiac muscle cell proliferation / ureteric bud development / endocardial cushion morphogenesis / organ growth / adrenal gland development / negative regulation of SMAD protein signal transduction / roof of mouth development / SMAD binding / TGF-beta receptor signaling activates SMADs / somatic stem cell population maintenance / R-SMAD binding / mesoderm formation / negative regulation of BMP signaling pathway / positive regulation of Wnt signaling pathway / phosphatase binding / negative regulation of osteoblast differentiation / anatomical structure morphogenesis / cell fate commitment / positive regulation of DNA binding / cis-regulatory region sequence-specific DNA binding / FOXO-mediated transcription of oxidative stress, metabolic and neuronal genes / skeletal muscle fiber development / positive regulation of epithelial to mesenchymal transition / negative regulation of fibroblast proliferation / gastrulation / transcription repressor complex / Downregulation of TGF-beta receptor signaling / transforming growth factor beta receptor signaling pathway / post-embryonic development / neural tube closure / cell motility / cellular response to glucose stimulus / Downregulation of SMAD2/3:SMAD4 transcriptional activity / SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription / negative regulation of transforming growth factor beta receptor signaling pathway / lung development / tau protein binding / PML body / DNA-binding transcription repressor activity, RNA polymerase II-specific / disordered domain specific binding / retina development in camera-type eye / double-stranded DNA binding / DNA-binding transcription activator activity, RNA polymerase II-specific Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Purkiss, A.G. / Kjaer, S. / George, R. / Hill, C.S. | ||||||
![]() | ![]() Title: Mutations in SKI in Shprintzen-Goldberg syndrome lead to attenuated TGF-beta responses through SKI stabilization. Authors: Gori, I. / George, R. / Purkiss, A.G. / Strohbuecker, S. / Randall, R.A. / Ogrodowicz, R. / Carmignac, V. / Faivre, L. / Joshi, D. / Kjaer, S. / Hill, C.S. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 124.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 86.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 474.9 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 477.8 KB | Display | |
Data in XML | ![]() | 12.9 KB | Display | |
Data in CIF | ![]() | 17.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1khxS S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||||||
Unit cell |
| ||||||||||||
Components on special symmetry positions |
|
-
Components
-Protein / Protein/peptide , 2 types, 2 molecules AB
#1: Protein | Mass: 25540.670 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|---|
#2: Protein/peptide | Mass: 3846.287 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
-Non-polymers , 4 types, 89 molecules ![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/TAR.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/TAR.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | #4: Chemical | ChemComp-GOL / #5: Chemical | #6: Water | ChemComp-HOH / | |
---|
-Details
Has ligand of interest | N |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.449 Å3/Da / Density % sol: 49.809 % / Description: Cubic crystals in phase separation droplets |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.6 Details: 1.5M Ammonium Sulphate, 0.15M Sodium Potassium Tartrate, 0.08M Tri-Sodium Citrate pH 5.6, 25% v/v Glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Oct 25, 2017 / Details: Mirrors |
Radiation | Monochromator: Double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9686 Å / Relative weight: 1 |
Reflection | Resolution: 2.03→56.91 Å / Num. obs: 15983 / % possible obs: 99.95 % / Redundancy: 10 % / Biso Wilson estimate: 37.09 Å2 / CC1/2: 0.998 / CC star: 1 / Rmerge(I) obs: 0.125 / Rpim(I) all: 0.04167 / Rrim(I) all: 0.1319 / Net I/σ(I): 12.24 |
Reflection shell | Resolution: 2.031→2.104 Å / Redundancy: 10.1 % / Rmerge(I) obs: 1.469 / Mean I/σ(I) obs: 1.89 / Num. unique obs: 1565 / CC1/2: 0.635 / CC star: 0.881 / Rpim(I) all: 0.4846 / Rrim(I) all: 1.547 / % possible all: 100 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 1khx Resolution: 2.03→56.91 Å / SU ML: 0.1886 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 26.7317 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.13 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.03→56.91 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
|