Type: oligosaccharide, Oligosaccharide / Class: Antigen / Mass: 529.490 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: oligosaccharide with branches / References: Lewis A antigen, beta anomer
Type: oligosaccharide, Oligosaccharide / Class: Antigen / Mass: 691.630 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: oligosaccharide with branches / References: Lewis A antigen, beta anomer
Mass: 18.015 Da / Num. of mol.: 673 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.31 Å3/Da / Density % sol: 46.75 %
Crystal grow
Temperature: 292 K / Method: vapor diffusion, hanging drop Details: 1.6 M AMSO4, 100 mM trisodium pH 5.6. Crystal flashed freezed in liquid nitrogen after transfer in 2.5 M Li2SO4
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N