+Open data
-Basic information
Entry | Database: PDB / ID: 6zta | ||||||
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Title | X-ray structure of mutated arabinofuranosidase | ||||||
Components | Alpha-L-arabinofuranosidase | ||||||
Keywords | HYDROLASE / Transferase | ||||||
Function / homology | Alpha-L-arabinofuranosidase, C-terminal / Alpha-L-arabinofuranosidase C-terminal domain / Alpha-L-arabinofuranosidase C-terminus / L-arabinose metabolic process / non-reducing end alpha-L-arabinofuranosidase / alpha-L-arabinofuranosidase activity / Glycosyl hydrolase, all-beta / Glycoside hydrolase superfamily / non-reducing end alpha-L-arabinofuranosidase Function and homology information | ||||||
Biological species | Thermobacillus xylanilyticus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å | ||||||
Authors | Tandrup, T. / Lo Leggio, L. / Zhao, J. / Bissaro, B. / Barbe, S. / Andre, I. / Dumon, C. / O'Donohue, M.J. / Faure, R. | ||||||
Citation | Journal: N Biotechnol / Year: 2021 Title: Probing the determinants of the transglycosylation/hydrolysis partition in a retaining alpha-l-arabinofuranosidase. Authors: Zhao, J. / Tandrup, T. / Bissaro, B. / Barbe, S. / Poulsen, J.N. / Andre, I. / Dumon, C. / Lo Leggio, L. / O'Donohue, M.J. / Faure, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6zta.cif.gz | 297 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6zta.ent.gz | 242.9 KB | Display | PDB format |
PDBx/mmJSON format | 6zta.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6zta_validation.pdf.gz | 449.6 KB | Display | wwPDB validaton report |
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Full document | 6zta_full_validation.pdf.gz | 469.4 KB | Display | |
Data in XML | 6zta_validation.xml.gz | 50.5 KB | Display | |
Data in CIF | 6zta_validation.cif.gz | 68.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zt/6zta ftp://data.pdbj.org/pub/pdb/validation_reports/zt/6zta | HTTPS FTP |
-Related structure data
Related structure data | 6zt6C 6zt7C 6zt8C 6zt9C 2vrqS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Beg auth comp-ID: ALA / Beg label comp-ID: ALA / End auth comp-ID: GLY / End label comp-ID: GLY / Refine code: _ / Auth seq-ID: 4 - 496 / Label seq-ID: 4 - 496
NCS ensembles :
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-Components
#1: Protein | Mass: 56273.418 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Thermobacillus xylanilyticus (bacteria) Gene: AbjA / Production host: Escherichia coli (E. coli) References: UniProt: O69262, non-reducing end alpha-L-arabinofuranosidase |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.94 Å3/Da / Density % sol: 68.8 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.9 / Details: 0.2 M sodium thiocyanate pH 6.9, 20% (v/v) PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 0.976 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 4, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 50293 / % possible obs: 99.9 % / Redundancy: 26.55 % / CC1/2: 0.985 / Net I/σ(I): 4.81 |
Reflection shell | Resolution: 3.1→3.18 Å / Num. unique obs: 3668 / CC1/2: 0.501 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2VRQ Resolution: 3.1→49.01 Å / Cor.coef. Fo:Fc: 0.838 / Cor.coef. Fo:Fc free: 0.813 / SU B: 68.64 / SU ML: 0.924 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 2.353 / ESU R Free: 0.569 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 176.23 Å2 / Biso mean: 86.88 Å2 / Biso min: 36.49 Å2
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Refinement step | Cycle: final / Resolution: 3.1→49.01 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05
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LS refinement shell | Resolution: 3.1→3.18 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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