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Open data
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Basic information
Entry | Database: PDB / ID: 6zt7 | ||||||
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Title | X-ray structure of mutated arabinofuranosidase | ||||||
![]() | Alpha-L-arabinofuranosidase | ||||||
![]() | HYDROLASE / Transferase | ||||||
Function / homology | ![]() L-arabinose metabolic process / non-reducing end alpha-L-arabinofuranosidase / alpha-L-arabinofuranosidase activity / polysaccharide catabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tandrup, T. / Lo Leggio, L. / Zhao, J. / Bissaro, B. / Barbe, S. / Andre, I. / Dumon, C. / O'Donohue, M.J. / Faure, R. | ||||||
![]() | ![]() Title: Probing the determinants of the transglycosylation/hydrolysis partition in a retaining alpha-l-arabinofuranosidase. Authors: Zhao, J. / Tandrup, T. / Bissaro, B. / Barbe, S. / Poulsen, J.N. / Andre, I. / Dumon, C. / Lo Leggio, L. / O'Donohue, M.J. / Faure, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 351.1 KB | Display | ![]() |
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PDB format | ![]() | 285.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.2 MB | Display | ![]() |
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Full document | ![]() | 2.3 MB | Display | |
Data in XML | ![]() | 73.7 KB | Display | |
Data in CIF | ![]() | 107.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6zt6C ![]() 6zt8C ![]() 6zt9C ![]() 6ztaC ![]() 2vrqS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Beg auth comp-ID: VAL / Beg label comp-ID: VAL / End auth comp-ID: GLY / End label comp-ID: GLY / Refine code: _ / Auth seq-ID: 3 - 496 / Label seq-ID: 3 - 496
NCS ensembles :
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Components
-Protein / Sugars , 2 types, 6 molecules ABC![](data/chem/img/AHR.gif)
![](data/chem/img/AHR.gif)
#1: Protein | Mass: 56155.215 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: AbjA / Production host: ![]() ![]() References: UniProt: O69262, non-reducing end alpha-L-arabinofuranosidase #5: Sugar | |
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-Non-polymers , 4 types, 1389 molecules ![](data/chem/img/ACT.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/BTB.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MPD.gif)
![](data/chem/img/BTB.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ACT / #3: Chemical | ChemComp-MPD / ( #4: Chemical | ChemComp-BTB / | #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.99 Å3/Da / Density % sol: 69.14 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 0.2 M ammonium acetate, 0.1 M Bis-tris pH 5.5, 45% (v/v) MPD |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 12, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.969 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→135.79 Å / Num. obs: 231584 / % possible obs: 99.7 % / Redundancy: 19.02 % / CC1/2: 0.998 / Net I/σ(I): 14.17 |
Reflection shell | Resolution: 1.85→1.9 Å / Num. unique obs: 16556 / CC1/2: 0.576 / % possible all: 97.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2VRQ Resolution: 1.85→135.79 Å / Cor.coef. Fo:Fc: 0.977 / Cor.coef. Fo:Fc free: 0.97 / SU B: 2.003 / SU ML: 0.057 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.075 / ESU R Free: 0.076 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 139.91 Å2 / Biso mean: 33.605 Å2 / Biso min: 14.26 Å2
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Refinement step | Cycle: final / Resolution: 1.85→135.79 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Rms dev position: 0.06 Å / Weight position: 0.05
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LS refinement shell | Resolution: 1.85→1.898 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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