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- PDB-6zj7: Trehalose transferase (TreT) from Thermoproteus uzoniensis soaked... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6zj7 | ||||||
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Title | Trehalose transferase (TreT) from Thermoproteus uzoniensis soaked with Mg | ||||||
![]() | Trehalose phosphorylase/synthase | ||||||
![]() | TRANSFERASE / trehalose transferase / retaining glycosyltransferase / glycosidic bond formation | ||||||
Function / homology | : / Trehalose synthase, N-terminal domain / Glycosyl transferase, family 1 / Glycosyl transferases group 1 / glycosyltransferase activity / glucose metabolic process / THIOCYANATE ION / Trehalose phosphorylase/synthase![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bento, I. / Mestrom, L. / Marsden, S.R. / van der Eijk, H. / Laustsen, J.U. / Jeffries, C.M. / Svergun, D.I. / Hagedoorn, P.-H. / Hanefeld, U. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Anomeric Selectivity of Trehalose Transferase with Rare l-Sugars. Authors: Mestrom, L. / Marsden, S.R. / van der Eijk, H. / Laustsen, J.U. / Jeffries, C.M. / Svergun, D.I. / Hagedoorn, P.L. / Bento, I. / Hanefeld, U. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 171.2 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 457 KB | Display | ![]() |
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Full document | ![]() | 458.6 KB | Display | |
Data in XML | ![]() | 16.6 KB | Display | |
Data in CIF | ![]() | 22.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6zj4C ![]() 6zjhC ![]() 6zmzC ![]() 6zn1C C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 45503.316 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: 768-20 / Gene: TUZN_0976 / Production host: ![]() ![]() | ||||||||
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#2: Chemical | ChemComp-SCN / #3: Chemical | ChemComp-PG4 / | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.55 Å3/Da / Density % sol: 51.7 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion / pH: 6.5 Details: Bis-Tris Propane, PEG 3350, potassium thiocyanate, PEG 400 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 2, 2019 |
Radiation | Monochromator: double-crystal monochromator FMB-OXFORD (Oxford, UK) Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→110.45 Å / Num. obs: 25646 / % possible obs: 98.8 % / Redundancy: 7.8 % / CC1/2: 0.999 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.039 / Rrim(I) all: 0.081 / Net I/σ(I): 17.1 |
Reflection shell | Resolution: 2.15→2.21 Å / Redundancy: 8.1 % / Rmerge(I) obs: 0.024 / Num. unique obs: 2477 / CC1/2: 0.7 / Rpim(I) all: 0.013 / Rrim(I) all: 0.028 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: apo-TreT Resolution: 2.15→58.757 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.935 / WRfactor Rfree: 0.257 / WRfactor Rwork: 0.193 / SU B: 7.635 / SU ML: 0.188 / Average fsc free: 0.862 / Average fsc work: 0.8837 / Cross valid method: FREE R-VALUE / ESU R: 0.237 / ESU R Free: 0.211 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.554 Å2
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Refinement step | Cycle: LAST / Resolution: 2.15→58.757 Å
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Refine LS restraints |
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LS refinement shell |
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