分子量: 53089.223 Da / 分子数: 1 変異: S83G,A86L,T101R,H103D,H105Y,L119F,M121L,E124D,R143K,D150E,A161V,R167L,R213L,V234L,K235R,V240L,I253A,I260A,N262R,K263R,H305R,C332V,F342A,T354S,F358V,S362A 由来タイプ: 組換発現 詳細: Residues 61-371 represent the rat neurotensin receptor 1 mutant H4 (NTSR1-H4). Residues 273 to 290 were deleted for crystallisation purposes. Residues 372 to 380 form a linker connecting ...詳細: Residues 61-371 represent the rat neurotensin receptor 1 mutant H4 (NTSR1-H4). Residues 273 to 290 were deleted for crystallisation purposes. Residues 372 to 380 form a linker connecting NTSR1 with the DARPin crystallisation chaperone. Residues 381 to 536 represent the DARPin crystallisation chaperone that is related to 5LW2. Residues 537 to 539 represent a short linker connecting the DARPin crystallisation chaperone with the HRV 3C protease recognition sequence. Residues 540 to 544 are part of the HRV 3C protease recognition sequence visible in the electron density. 由来: (組換発現) Rattus norvegicus (ドブネズミ), (組換発現) synthetic construct (人工物) 遺伝子: Ntsr1, Ntsr / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P20789
解像度: 2.923→28.307 Å / Cor.coef. Fo:Fc: 0.865 / Cor.coef. Fo:Fc free: 0.833 / SU B: 29.052 / SU ML: 0.481 / 交差検証法: FREE R-VALUE / ESU R Free: 0.659 / 詳細: Hydrogens have been added in their riding positions
Rfactor
反射数
%反射
Rfree
0.3069
440
5.076 %
Rwork
0.2806
8229
-
all
0.282
-
-
obs
-
8669
60.335 %
溶媒の処理
イオンプローブ半径: 0.7 Å / 減衰半径: 0.7 Å / VDWプローブ半径: 1.1 Å / 溶媒モデル: MASK BULK SOLVENT