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- PDB-6z3m: Repulsive Guidance Molecule B (RGMB) in complex with Growth Diffe... -

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Basic information

Entry
Database: PDB / ID: 6z3m
TitleRepulsive Guidance Molecule B (RGMB) in complex with Growth Differentiation Factor 5 (GDF5) and Neogenin 1 (NEO1).
Components
  • Growth/differentiation factor 5
  • Neogenin
  • RGM domain family member B
KeywordsSIGNALING PROTEIN / Repulsive Guidance Molecule / RGM / Bone Morphogenetic Protein / BMP / Growth Differentiation Factor 5 / GDF5 / Neogenin / axon guidance / TGFbeta signalling / brain development / iron metabolism
Function / homology
Function and homology information


ossification involved in bone remodeling / trans-synaptic signaling, modulating synaptic transmission / forelimb morphogenesis / BMP binding / chondroblast differentiation / hindlimb morphogenesis / negative regulation of axon regeneration / negative regulation of mesenchymal cell apoptotic process / Netrin-1 signaling / positive regulation of chondrocyte differentiation ...ossification involved in bone remodeling / trans-synaptic signaling, modulating synaptic transmission / forelimb morphogenesis / BMP binding / chondroblast differentiation / hindlimb morphogenesis / negative regulation of axon regeneration / negative regulation of mesenchymal cell apoptotic process / Netrin-1 signaling / positive regulation of chondrocyte differentiation / co-receptor binding / mesenchymal cell apoptotic process / BMP receptor binding / regulation of axon regeneration / plasma membrane protein complex / positive regulation of BMP signaling pathway / myoblast fusion / negative regulation of chondrocyte differentiation / embryonic limb morphogenesis / Molecules associated with elastic fibres / intracellular vesicle / endoplasmic reticulum-Golgi intermediate compartment / protein secretion / positive regulation of SMAD protein signal transduction / regulation of multicellular organism growth / negative regulation of protein secretion / chondrocyte differentiation / BMP signaling pathway / response to mechanical stimulus / coreceptor activity / side of membrane / positive regulation of neuron differentiation / transforming growth factor beta receptor signaling pathway / cytokine activity / growth factor activity / postsynaptic density membrane / multicellular organismal-level iron ion homeostasis / negative regulation of epithelial cell proliferation / cell-cell signaling / signaling receptor activity / growth cone / negative regulation of neuron apoptotic process / intracellular iron ion homeostasis / cell adhesion / cadherin binding / membrane raft / regulation of DNA-templated transcription / positive regulation of DNA-templated transcription / glutamatergic synapse / cell surface / Golgi apparatus / signal transduction / extracellular space / extracellular region / nucleoplasm / identical protein binding / membrane / plasma membrane
Similarity search - Function
Repulsive guidance molecule, C-terminal / Repulsive guidance molecule, N-terminal / Repulsive guidance molecule / Repulsive guidance molecule (RGM) C-terminus / Repulsive guidance molecule (RGM) N-terminus / Neogenin, C-terminal / Neogenin C-terminus / TGF-beta, propeptide / TGF-beta propeptide / Transforming growth factor beta, conserved site ...Repulsive guidance molecule, C-terminal / Repulsive guidance molecule, N-terminal / Repulsive guidance molecule / Repulsive guidance molecule (RGM) C-terminus / Repulsive guidance molecule (RGM) N-terminus / Neogenin, C-terminal / Neogenin C-terminus / TGF-beta, propeptide / TGF-beta propeptide / Transforming growth factor beta, conserved site / TGF-beta family signature. / Transforming growth factor-beta-related / Transforming growth factor-beta (TGF-beta) family / Transforming growth factor-beta, C-terminal / Transforming growth factor beta like domain / TGF-beta family profile. / Immunoglobulin domain / Cystine-knot cytokine / Immunoglobulin I-set / Immunoglobulin I-set domain / Fibronectin type III domain / Fibronectin type 3 domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Fibronectin type-III domain profile. / Fibronectin type III / Fibronectin type III superfamily / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Growth/differentiation factor 5 / Neogenin / Repulsive guidance molecule B
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 5.501 Å
AuthorsMalinauskas, T. / Peer, T.V. / Bishop, B. / Muller, T.D. / Siebold, C.
Funding support United Kingdom, European Union, Germany, France, 7items
OrganizationGrant numberCountry
Cancer Research UKC20724/A14414 United Kingdom
Cancer Research UKC20724/A26752 United Kingdom
European Research Council (ERC)647278European Union
German Research Foundation (DFG)MU1095/3-2 Germany
German Research Foundation (DFG)MU1095/5-1 Germany
Wellcome Trust203852/Z/16/2 United Kingdom
Human Frontier Science Program (HFSP)LT000021/2014-L France
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2020
Title: Repulsive guidance molecules lock growth differentiation factor 5 in an inhibitory complex.
Authors: Malinauskas, T. / Peer, T.V. / Bishop, B. / Mueller, T.D. / Siebold, C.
History
DepositionMay 21, 2020Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jul 1, 2020Provider: repository / Type: Initial release
Revision 1.1Jul 8, 2020Group: Database references / Category: citation
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Jul 15, 2020Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Jul 29, 2020Group: Data collection / Derived calculations / Structure summary
Category: chem_comp / entity ...chem_comp / entity / pdbx_chem_comp_identifier / pdbx_entity_nonpoly / struct_conn / struct_site / struct_site_gen
Item: _chem_comp.name / _entity.pdbx_description ..._chem_comp.name / _entity.pdbx_description / _pdbx_entity_nonpoly.name / _struct_conn.pdbx_role
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 1.4May 24, 2023Group: Database references / Structure summary / Category: chem_comp / database_2 / struct
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct.title
Revision 1.5Jul 26, 2023Group: Author supporting evidence / Other / Category: pdbx_audit_support / pdbx_database_status
Item: _pdbx_audit_support.country / _pdbx_database_status.pdb_format_compatible
Revision 1.6Feb 7, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.7Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Growth/differentiation factor 5
B: Growth/differentiation factor 5
C: RGM domain family member B
D: RGM domain family member B
E: Neogenin
F: Neogenin
G: Growth/differentiation factor 5
H: Growth/differentiation factor 5
I: RGM domain family member B
J: RGM domain family member B
K: Neogenin
L: Neogenin
M: Growth/differentiation factor 5
N: Growth/differentiation factor 5
O: RGM domain family member B
P: RGM domain family member B
Q: Neogenin
R: Neogenin
c: RGM domain family member B
d: RGM domain family member B
i: RGM domain family member B
j: RGM domain family member B
o: RGM domain family member B
p: RGM domain family member B
S: RGM domain family member B
T: RGM domain family member B
U: RGM domain family member B
V: RGM domain family member B
W: RGM domain family member B
X: RGM domain family member B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)989,27439
Polymers987,28430
Non-polymers1,9919
Water00
1
A: Growth/differentiation factor 5
B: Growth/differentiation factor 5
C: RGM domain family member B
D: RGM domain family member B
E: Neogenin
F: Neogenin
c: RGM domain family member B
d: RGM domain family member B
S: RGM domain family member B
T: RGM domain family member B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)329,75813
Polymers329,09510
Non-polymers6643
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
G: Growth/differentiation factor 5
H: Growth/differentiation factor 5
I: RGM domain family member B
J: RGM domain family member B
K: Neogenin
L: Neogenin
i: RGM domain family member B
j: RGM domain family member B
U: RGM domain family member B
V: RGM domain family member B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)329,75813
Polymers329,09510
Non-polymers6643
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
M: Growth/differentiation factor 5
N: Growth/differentiation factor 5
O: RGM domain family member B
P: RGM domain family member B
Q: Neogenin
R: Neogenin
o: RGM domain family member B
p: RGM domain family member B
W: RGM domain family member B
X: RGM domain family member B
hetero molecules


Theoretical massNumber of molelcules
Total (without water)329,75813
Polymers329,09510
Non-polymers6643
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)279.480, 279.480, 142.370
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number171
Space group name H-MP62

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Components

#1: Protein
Growth/differentiation factor 5 / GDF-5 / Bone morphogenetic protein 14 / BMP-14 / Cartilage-derived morphogenetic protein 1 / CDMP-1 ...GDF-5 / Bone morphogenetic protein 14 / BMP-14 / Cartilage-derived morphogenetic protein 1 / CDMP-1 / Lipopolysaccharide-associated protein 4 / LPS-associated protein 4 / Radotermin


Mass: 13405.481 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GDF5, BMP14, CDMP1 / Production host: Escherichia coli (E. coli) / References: UniProt: P43026
#2: Protein
RGM domain family member B / DRG11-responsive axonal guidance and outgrowth of neurite / DRAGON


Mass: 41036.719 Da / Num. of mol.: 18
Source method: isolated from a genetically manipulated source
Details: Sequence alignment is misleading. / Source: (gene. exp.) Homo sapiens (human) / Gene: RGMB / Cell line (production host): HEK293T / Production host: Homo sapiens (human) / References: UniProt: Q6NW40
#3: Protein
Neogenin


Mass: 28031.631 Da / Num. of mol.: 6
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Neo1, Ngn / Cell line (production host): HEK293T / Production host: Homo sapiens (human) / References: UniProt: P97798
#4: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 9
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6 / Feature type: SUBJECT OF INVESTIGATION
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0
Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.41 Å3/Da / Density % sol: 72.11 %
Crystal growTemperature: 294 K / Method: vapor diffusion, sitting drop / Details: 0.1 M NaCl, 20 mM MES pH 6.7, 6.6% w/v PEG 4000 / PH range: 6.7-7.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04 / Wavelength: 0.9795 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 15, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9795 Å / Relative weight: 1
ReflectionResolution: 5.5→70.19 Å / Num. obs: 20764 / % possible obs: 99.8 % / Redundancy: 6.3 % / Rmerge(I) obs: 0.253 / Net I/σ(I): 5.7
Reflection shellResolution: 5.5→5.64 Å / Mean I/σ(I) obs: 0.8 / Num. unique obs: 1499 / CC1/2: 0.278 / Rpim(I) all: 1.103

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Processing

Software
NameVersionClassification
PHENIX(1.16_3549: ???)refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6Z3J

6z3j


Resolution: 5.501→69.87 Å / SU ML: 2.21 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 61.12 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.4282 1061 5.13 %
Rwork0.3257 --
obs0.331 20690 99.62 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 5.501→69.87 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms25084 0 126 0 25210
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01225781
X-RAY DIFFRACTIONf_angle_d1.48935040
X-RAY DIFFRACTIONf_dihedral_angle_d15.2849509
X-RAY DIFFRACTIONf_chiral_restr0.114003
X-RAY DIFFRACTIONf_plane_restr0.0074479
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
5.501-5.7510.48911380.4292412X-RAY DIFFRACTION100
5.751-6.05410.43981410.43352440X-RAY DIFFRACTION100
6.0541-6.43320.46831300.42342441X-RAY DIFFRACTION100
6.4332-6.92950.46931140.39952471X-RAY DIFFRACTION100
6.9295-7.6260.48181380.36222449X-RAY DIFFRACTION100
7.626-8.72770.40061470.31652447X-RAY DIFFRACTION100
8.7277-10.98910.37911290.26792468X-RAY DIFFRACTION100
10.9891-69.870.43321240.2872501X-RAY DIFFRACTION98
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.37081.44340.43035.37082.55381.23841.5862-2.33163.7477-0.13771.4373-1.92370.88584.6171.42936.7733-2.74331.02322.9315-1.37883.9921-86.353360.0825-49.0632
22.20321.66151.27376.4573-4.6046.46033.05-1.199-1.3581-2.5359-1.0729-2.16075.24293.38151.67396.0427-0.2422-0.3622.0623-0.20184.616-95.727746.8803-52.7452
30.6821-0.8602-0.07651.24460.38553.1109-0.21820.36680.80030.1550.3394-1.20871.95793.1519-1.90267.6196-3.3079-2.13448.686-2.89640.8333-80.761962.3918-72.4917
44.6389-2.97240.69434.5286-4.99917.3378-0.26146.2847-1.2702-0.7644-2.0278-1.55870.0342.5268-3.57018.4683-1.8265-3.70747.7379-1.162.769-89.752336.7849-31.0677
58.3986-0.91622.78012.98353.07815.01431.298-0.8361-0.89662.96731.82861.47892.4652-4.1962-0.97146.5125-1.11623.15326.35510.74257.96576.532955.03240.9143
60.4745-1.236-1.0972.99643.01533.0205-5.1123.0281-1.11463.64171.50243.81590.158-1.78811.41738.3522-0.21481.21284.39081.00215.9011-9.433654.528844.7777
72.37681.60490.59230.97760.31120.08340.89550.46411.5946-0.3482-1.49162.5769-4.5669-2.6558-5.94595.03722.37682.31448.1808-3.89534.884511.920865.431362.5068
88.43940.6564-1.38093.0359-1.67411.02814.13540.2413-1.7579-1.351.923-2.05591.0233-0.37756.10167.5586-3.0592-1.11798.6855-3.65452.8672-15.185656.68121.7393
95.7041.2945-5.17175.03941.06386.1744-1.03581.3276-0.62120.69751.33121.63560.61855.4722-0.38262.5833-0.2556-0.18575.58690.71434.4663-132.625325.223349.4315
104.1788-4.15760.43597.6818-3.66466.3649-0.1019-1.28-2.49761.3203-0.4130.6019-1.0076-1.52990.6121.89320.5917-0.97985.4667-1.5475.6813-148.830125.370445.7152
116.38280.39151.69195.8408-2.12481.3233-4.76750.11-4.26192.1899-0.37611.30443.4682-7.35971.53245.9628-0.95461.97457.7394-0.25493.9825-127.422916.030927.7049
127.34190.41697.01518.68293.12667.6204-0.0629-4.3852-1.96212.79613.4150.20450.17860.4904-1.15082.8322-0.24890.75536.9085-1.9134.6342-154.116521.098269.399
133.55793.4888-2.1727.8084-3.50017.9433-3.82723.2987-4.0562-1.37222.2891-2.52141.12031.21920.44043.1303-2.07990.65055.3399-0.46942.2979-51.5028108.1154-60.4394
142.8768-0.18340.95172.87990.5250.533-1.286-0.86040.00121.5826-0.97532.1725-1.7199-0.5495-0.53875.9613-2.68542.42334.7354-2.20172.735-89.6949113.4077-39.0899
150.7699-0.8898-2.13537.4208-0.81437.77650.8073-2.00691.84180.210.5141-6.12243.30961.238-0.9612.7621-0.3201-0.04282.8573-0.91415.1905-122.3601-5.1213-41.9146
160.08230.0457-1.00544.80320.2217.9315-0.09712.7104-2.1526-1.3653-2.18291.5420.6698-1.04831.67814.03190.175-0.69682.3714-1.43186.5074-147.07228.8298-55.4192
172.35341.914-2.06711.3227-1.43451.57541.7486-3.7225-0.88461.1807-0.22381.6776-1.2222-0.4851-0.66755.66883.74032.60068.66841.1037.397565.122658.392453.7267
188.53015.3444.67586.24440.4434.47270.73530.5169-4.6186-4.06281.0773-2.31641.53730.0192-1.50127.40032.8571.38677.5280.01165.821347.845820.621739.105
198.72514.2911-4.21764.85871.5096.80991.9879-2.4589-2.4399-1.9318-2.5409-2.7269-0.5469-1.56021.25827.6538-3.08392.48136.6973-1.32785.2256-68.077761.58334.4133
201.3526-0.17523.4496-0.0099-0.34138.2413-2.51880.44810.47210.03-0.9852-0.8609-2.45-2.78411.33845.9082-1.37482.74917.5617-2.66959.2019-51.90329.095156.5649
215.61710.9048-5.68828.2477-0.87066.94731.37483.193.0545-2.35760.24382.73031.2897-1.4069-1.15813.27-0.3549-1.32394.7387-0.04283.9758-72.995721.100137.0254
221.85920.6630.29435.8123-0.76573.8562-1.2284-3.67842.4463.51871.77432.27941.8199-0.06593.00451.84040.20710.94184.7005-1.663-1.2862-89.39659.314251.754
232.4345-1.775-0.86246.30587.5041.96785.05874.8675-1.01131.858-1.0811-2.14725.2014-0.3089-1.85994.94370.1369-1.20727.8998-1.1975.9097-206.175822.390653.5726
242.6343-0.952-1.62010.43470.78021.04441.3341.55241.0094-0.61790.8032-0.08121.7981-2.80821.68924.06370.1802-3.87299.5745-0.07885.8293-189.970460.228938.0324
252.036-0.6615-0.1378.54080.48883.461-1.3225-1.308-0.54972.0732.00892.52173.5885-0.49660.52126.4577-2.89273.07922.7408-1.37556.1429-83.226693.9878-47.8338
269.2401-4.2194.57578.0876-4.30314.18624.16471.82956.06651.3931-4.0659-0.0981-1.88391.7434-0.47113.19370.27250.43742.42090.93497.3023-125.210430.45-51.662
275.15596.11670.52867.4989-0.95554.00853.2924-1.8868-3.76253.7338-4.13880.0714-2.6806-4.9850.4785.61871.65830.43996.66931.76716.162235.68138.123444.7577
280.0421-0.0698-0.13070.09070.47911.17981.8367-1.72551.28280.48024.5669-0.50040.3655-1.873-2.40375.2959-1.7639-1.50126.82511.222511.501-39.838543.355349.5957
296.0941-3.6959-2.84615.5658-1.58444.92610.74932.9707-3.5356-2.9663-2.00312.68255.0561-4.97380.52283.9169-1.5591-1.14464.90190.0193.9375-101.995342.070646.2478
303.77012.6291-3.28719.76551.24124.25750.1183.1789-1.572.3953-0.6289-0.70080.40960.64130.29862.4881.3304-0.58028.05831.36647.0692-177.2542.706543.0237
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain 'A' and resid 397 through 501)
2X-RAY DIFFRACTION2(chain 'B' and resid 396 through 501)
3X-RAY DIFFRACTION3(chain 'C' and resid 53 through 127)
4X-RAY DIFFRACTION4(chain 'D' and resid 1 through 127)
5X-RAY DIFFRACTION5(chain 'G' and resid 396 through 501)
6X-RAY DIFFRACTION6(chain 'H' and resid 397 through 501)
7X-RAY DIFFRACTION7(chain 'I' and resid 1 through 127)
8X-RAY DIFFRACTION8(chain 'J' and resid 53 through 126)
9X-RAY DIFFRACTION9(chain 'M' and resid 397 through 501)
10X-RAY DIFFRACTION10(chain 'N' and resid 396 through 501)
11X-RAY DIFFRACTION11(chain 'O' and resid 53 through 127)
12X-RAY DIFFRACTION12(chain 'P' and resid 1 through 127)
13X-RAY DIFFRACTION13(chain 'E' and resid 884 through 983)
14X-RAY DIFFRACTION14(chain 'E' and resid 984 through 1088)
15X-RAY DIFFRACTION15(chain 'F' and resid 884 through 983)
16X-RAY DIFFRACTION16(chain 'F' and resid 984 through 1088)
17X-RAY DIFFRACTION17(chain 'K' and resid 884 through 983)
18X-RAY DIFFRACTION18(chain 'K' and resid 984 through 1088)
19X-RAY DIFFRACTION19(chain 'L' and resid 884 through 983)
20X-RAY DIFFRACTION20(chain 'L' and resid 984 through 1088)
21X-RAY DIFFRACTION21(chain 'Q' and resid 884 through 983)
22X-RAY DIFFRACTION22(chain 'Q' and resid 984 through 1088)
23X-RAY DIFFRACTION23(chain 'R' and resid 884 through 983)
24X-RAY DIFFRACTION24(chain 'R' and resid 984 through 1088)
25X-RAY DIFFRACTION25(chain 'c' and resid 138 through 321)
26X-RAY DIFFRACTION26(chain 'd' and resid 138 through 321)
27X-RAY DIFFRACTION27(chain 'i' and resid 138 through 321)
28X-RAY DIFFRACTION28(chain 'j' and resid 138 through 321)
29X-RAY DIFFRACTION29(chain 'o' and resid 138 through 321)
30X-RAY DIFFRACTION30(chain 'p' and resid 138 through 321)

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