+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6z12 | ||||||||||||
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タイトル | Salmonella AcrB solubilised in the SMA copolymer | ||||||||||||
要素 | Efflux pump membrane transporter | ||||||||||||
キーワード | MEMBRANE PROTEIN / AcrB | ||||||||||||
機能・相同性 | 機能・相同性情報 xenobiotic transport / efflux transmembrane transporter activity / membrane => GO:0016020 / plasma membrane 類似検索 - 分子機能 | ||||||||||||
生物種 | Salmonella enterica I (サルモネラ菌) | ||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 4.6 Å | ||||||||||||
データ登録者 | Muench, s.p. / Johnson, R.M. | ||||||||||||
資金援助 | 英国, 3件
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引用 | ジャーナル: Microorganisms / 年: 2020 タイトル: Cryo-EM Structure and Molecular Dynamics Analysis of the Fluoroquinolone Resistant Mutant of the AcrB Transporter from . 著者: Rachel M Johnson / Chiara Fais / Mayuriben Parmar / Harish Cheruvara / Robert L Marshall / Sophie J Hesketh / Matthew C Feasey / Paolo Ruggerone / Attilio V Vargiu / Vincent L G Postis / ...著者: Rachel M Johnson / Chiara Fais / Mayuriben Parmar / Harish Cheruvara / Robert L Marshall / Sophie J Hesketh / Matthew C Feasey / Paolo Ruggerone / Attilio V Vargiu / Vincent L G Postis / Stephen P Muench / Vassiliy N Bavro / 要旨: is an important genus of Gram-negative pathogens, treatment of which has become problematic due to increases in antimicrobial resistance. This is partly attributable to the overexpression of ... is an important genus of Gram-negative pathogens, treatment of which has become problematic due to increases in antimicrobial resistance. This is partly attributable to the overexpression of tripartite efflux pumps, particularly the constitutively expressed AcrAB-TolC. Despite its clinical importance, the structure of the AcrB transporter remained unknown to-date, with much of our structural understanding coming from the orthologue. Here, by taking advantage of the styrene maleic acid (SMA) technology to isolate membrane proteins with closely associated lipids, we report the very first experimental structure of AcrB transporter. Furthermore, this novel structure provides additional insight into mechanisms of drug efflux as it bears the mutation (G288D), originating from a clinical isolate of Typhimurium presenting an increased resistance to fluoroquinolones. Experimental data are complemented by state-of-the-art molecular dynamics (MD) simulations on both the wild type and G288D variant of AcrB. Together, these reveal several important differences with respect to the protein, providing insights into the role of the G288D mutation in increasing drug efflux and extending our understanding of the mechanisms underlying antibiotic resistance. | ||||||||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6z12.cif.gz | 932.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6z12.ent.gz | 781.1 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6z12.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/z1/6z12 ftp://data.pdbj.org/pub/pdb/validation_reports/z1/6z12 | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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-要素
#1: タンパク質 | 分子量: 113759.227 Da / 分子数: 3 / 由来タイプ: 組換発現 由来: (組換発現) Salmonella enterica I (サルモネラ菌) 遺伝子: acrB, A4O23_10955, A4O45_03810, A5895_15265, AAC70_12525, AAL03_14960, AAM89_07765, AAV76_19425, ABK55_05725, ABO08_03475, AC391_10965, ACH21_19020, ACK40_00105, ACK79_15085, ACS18_20650, ...遺伝子: acrB, A4O23_10955, A4O45_03810, A5895_15265, AAC70_12525, AAL03_14960, AAM89_07765, AAV76_19425, ABK55_05725, ABO08_03475, AC391_10965, ACH21_19020, ACK40_00105, ACK79_15085, ACS18_20650, ACV85_05805, ADQ53_03685, ADQ64_15580, ADT85_17470, AF726_08820, AG46_12710, AG53_15950, AGQ41_04335, AGQ90_03080, AGR09_08325, AGR46_16415, AH060_16145, AH079_04350, AH254_22600, AH360_16970, AH543_16315, AH557_09130, AH612_19020, AH874_13175, AHN36_15795, AHN91_21865, AHQ94_20600, AHS94_22030, AHU36_11045, AHU48_23190, AHX56_08240, AHY46_22370, AHZ72_05105, AIA22_12165, AIB97_16815, AID13_02815, AIE18_09140, AIF85_07160, AL864_02820, AL929_23130, ALX58_14045, AU885_11650, AVA95_03925, AVB28_08510, AVG51_11480, AVH38_04545, AXK79_19705, AXM38_07800, AXM50_14470, AXM56_16685, AXM65_18530, AXM93_08445, AXN14_02155, AXN15_07580, AXN21_06965, AXN39_11550, AXN51_12700, AXN72_08000, AXO05_08570, AXO78_17315, AXO93_15340, AXQ08_14655, AXR02_08970, AXR82_02315, AXR91_01290, AXS04_01695, AXS89_02055, AXT07_05965, AXT19_05915, B9C63_11810, BXE81_09065, BXG02_07180, BXG42_09370, BXH36_10215, BXH66_08040, BXL85_11765, BXO48_06645, BZ557_12735, BZ723_04100, BZ903_08940, BZV91_08875, BZW24_03920, BZW54_04755, C9E93_06140, C9E94_19180, C9F01_08965, C9F05_01290, C9P23_10335, C9Y45_03940, CB317_01000, CC394_01545, CC530_00775, CC828_04535, CE94_19610, CF93_04280, CPY04_22475, CQE41_07070, CQF73_09855, CQH69_04135, CQM91_23105, CSM01_01615, CSN39_10900, CSN48_19880, CSN51_17860, CSO96_14135, CSP66_16145, CST74_11845, CST78_12945, CV491_04375, CWG53_02155, CWG97_11285, D4346_01325, D4392_10020, D4396_04285, D4408_06485, D4427_06950, D4428_05215, D4444_11555, D4471_08450, D4490_01015, D4494_10470, D4507_05270, D4514_03660, D4515_01545, D4546_09390, D4551_09260, D4D97_05485, D4D98_08570, D4E01_09290, D4E37_05350, D4E64_10565, D4E75_00440, D4E90_05185, D4F17_01060, D4F19_04440, D4F20_07155, D4F29_07730, D4F49_16455, D4F54_04680, D4F77_10190, D4F87_02985, D4F98_10640, D4G07_10045, D4G10_11575, D4G50_05510, D4X66_01450, D4X67_01465, D4X81_08350, D4Y13_13605, D4Y27_04430, D4Y38_00790, D4Y42_06150, D4Y56_03795, D4Y61_08865, D4Y96_04575, D4Z14_05470, D4Z16_07845, D4Z17_03400, D4Z29_11545, D4Z41_08675, D4Z50_07970, D4Z57_03410, D4Z58_04655, D4Z75_04825, D5764_07825, D5769_02495, D5773_00875, D5782_03660, D5810_01285, D5836_17200, D5848_11770, D5856_03150, D5861_06225, D5881_04345, D5889_07675, D5895_01345, D5905_18015, D5915_03200, D5970_05735, D5972_06010, D5A61_03660, D5A65_05805, D5A76_04875, D5A82_03865, D5A94_12405, D5B23_01450, D5B60_01340, D5B66_05165, D5B94_03135, D5C00_05295, D5C12_08175, D5C21_07220, D5C32_06325, D5C34_10465, D5C40_07860, D5C41_08390, D5C46_12375, D5C64_03810, D5C69_08580, D5O45_04325, D5O48_05730, D5O74_04490, D5O78_09935, D5O80_07835, D5O86_11185, D5P09_09970, D5P22_01470, D5P31_04355, D5P36_04640, D5P42_03925, D5P52_00720, D5P54_05700, D5P59_05915, D5P71_08860, D5P75_03560, D5P94_16990, D5Q05_03935, D5Q09_06085, D5Q39_11635, D5Q43_00445, D5W53_06130, D5W81_01065, D5W91_06810, D5X16_03865, D5X20_07410, D5X39_00990, D5X56_02780, D5X61_00680, D5X72_03440, D5X88_07550, D5X91_04060, D5Y01_00825, D5Y06_08270, D5Y08_23240, D5Y41_01425, D6331_01310, D6379_03305, D6389_04215, D6413_06485, D6429_04170, D6448_15635, D6453_04205, D6455_06220, D6467_08685, D6J43_21925, D6J46_01025, D6J55_02800, D6J67_02330, D6J74_05385, D6J84_08940, D6K21_03765, D6K24_01080, D6K59_04410, D6L05_07630, D6P24_03240, D6P41_01305, D6P57_11975, D6P63_03960, D6Q07_11960, D6Q21_01005, D6Q25_01020, D6Q70_23040, D6Q87_10085, D6Q96_09190, D6Q97_04185, D6R14_09640, D6R58_05020, D6R62_09555, D6R98_02810, D6S02_04705, D6S13_01360, D6S22_05290, D6S36_05340, D6S45_01045, D6S47_07470, D6S50_05845, D6S52_07325, D6S74_01450, D6S83_06345, D6S89_03890, D6T18_03025, D6T31_08100, D6T37_07430, D7M89_05935, D7M98_11725, D7N03_02455, D7N07_08155, D7N22_06745, D7N26_22710, D7N30_05075, D7N47_05845, D7N51_15930, D7N52_04690, D7N80_02885, D7N86_17235, D7O13_06330, D7O17_07925, D7O23_11190, D7O25_01030, D7O28_08970, D7O59_06225, D7O77_04615, D8Q64_01020, D8Q73_05540, D8R14_01045, D8R18_08390, D8R62_10510, D8R78_07850, D8S31_02190, D8S34_08660, D8S35_08175, D8S37_06530, D8S38_08610, D8S62_04305, D8S66_05780, D9O60_02875, D9O71_05360, D9O80_03040, D9O86_13290, D9O95_03895, D9P04_07965, D9P12_05975, D9P13_14425, D9P19_07675, D9P28_09530, D9P30_03370, D9P43_10815, D9P51_02955, D9P55_07730, D9P58_06870, D9P68_09885, D9P88_00985, D9P92_02905, D9P97_06685, D9Q07_09260, D9Q11_05725, D9Q23_03710, D9Q29_06205, D9Q37_07930, D9Q49_08285, D9Q50_10325, DAR35_01005, DAW17_06730, DAX91_07060, DFQ85_05430, DFR01_09160, DK013_01025, DK030_06300, DK052_23015, DK083_04615, DK093_01040, DK094_01025, DK101_08130, DK113_08305, DK117_07485, DK127_09260, DK678_00070, DK681_09420, DK687_04055, DK711_01855, DK719_05700, DK739_06740, DK741_10685, DK756_03460, DK767_12285, DKI70_07030, DKJ03_09570, DKJ07_05835, DKS84_08230, DKT00_02710, DKT04_15920, DKT21_12805, DKT27_10265, DKT52_07220, DKU65_14915, DKV05_06000, DKV20_06200, DKV25_04445, DL088_11075, DL104_17055, DL110_05250, DL111_06640, DL115_01355, DL130_03140, DL146_02805, DLB02_07650, DLB18_08505, DLB23_01000, DLB36_10070, DLB43_07510, DLB46_07880, DLB62_03890, DLB73_00105, DLC24_04935, DLE90_18415, DLF22_04805, DLF29_05020, DLF30_08180, DLF33_10805, DLF35_01005, DLF36_02845, DLM06_04560, DLM09_00995, DLM10_09965, DLM30_04930, DLQ73_12300, DLQ79_01000, DLQ86_15735, DLQ92_03590, DLR05_06915, DLR06_01490, DLR25_05290, DLR57_10745, DM001_07440, DM006_09100, DM016_05255, DM323_06700, DM347_08405, DM358_08140, DM367_10780, DM371_02430, DM700_18145, DM703_02460, DM705_08310, DM743_09605, DMA85_03170, DMA89_15850, DMB03_08830, DMB04_15185, DML85_00605, DML97_09050, DMM16_01960, DMM17_11150, DMM22_02845, DMM24_11405, DMM43_07890, DMO61_03735, DMO65_01050, DMO79_09060, DMU74_10415, DMU80_09050, DMU82_12600, DMU94_09600, DMV06_03650, DMV13_03350, DMV20_20060, DMV32_00825, DMV34_02950, DMZ76_01020, DN020_06485, DN087_03575, DN092_04545, DN123_07690, DN125_01440, DN130_05830, DN132_04380, DN181_04005, DN182_06745, DN220_06100, DN241_03340, DN257_05145, DN259_05090, DN271_19320, DN282_15665, DN317_01415, DN319_10795, DN322_05135, DN358_10690, DN362_06885, DNI00_08045, DNI25_05135, DNI46_06020, DNI61_09285, DNI77_05260, DNI82_12125, DNI85_03675, DNJ05_06165, DNJ30_02305, DNL79_03545, DNM18_05050, DNM22_08605, DNM39_12310, DNM44_03995, DNM47_08685, DNM48_03895, DNM80_06910, DNN00_05420, DNU17_09140, DNU22_04660, DNU30_01285, DNU40_06770, DNU46_14660, DNU67_08335, DNU68_11160, DNU78_13165, DNU82_03725, DNU84_13710, DNU93_05320, DNV11_03960, DNV18_07230, DNV24_12015, DNV55_05965, DNV93_02810, DNW15_00865, DNY10_04300, DNY38_00420, DNY86_09760, DNZ14_01330, DNZ24_04550, DNZ41_01315, DNZ69_08840, DNZ74_07090, DNZ75_08705, DNZ81_03425, DNZ91_05745, DO557_05890, DO565_08085, DO591_06685, DO596_04935, DO617_04810, DO619_02490, DO630_04000, DO638_05650, DO649_13400, DO654_06280, DO661_06270, DO663_04365, DO672_01035, DO679_08555, DO681_04055, DO690_00395, DO696_07715, DO712_22550, DO727_09960, DO731_01530, DO771_06730, DO807_07800, DO827_06795, DO973_04300, DO981_01690, DO994_01335, DOA98_08020, DOB00_09740, DOB23_01440, DOB53_16610, DOB60_05740, DOB76_01635, DOC12_00300, DOC15_05765, DOC25_09930, DOC31_08415, DOC34_09315, DOC40_14960, DOC63_04280, DOG94_02935, DOH04_12155, DOH12_22545, DOH33_03235, DOH41_03550, DOH63_02830, DOH69_10710, DOH74_13625, DOH75_06000, DOH81_09930, DOI30_01020, DOI60_10480, DOI66_05845, DOJ02_05395, DOQ45_03150, DOQ73_06415, DOQ79_01065, DOR42_03375, DOR43_04775, DOR46_16150, DOR51_23780, DOR52_02535, DOR63_07395, DOR64_10515, DOR75_06845, DOV68_03030, DOV71_04785, DOV77_15350, DOV89_01625, DOW27_10165, DOW52_22585, DOW74_04075, DOX21_02055, DOX30_03420, DP688_13895, DP693_00825, DP698_06830, DP703_05145, DP712_02970, DP713_03380, DP716_06840, DP727_22840, DP728_16275, DP736_07365, DP748_02490, DP766_03155, DP778_03250, DP792_05710, DP800_16220, DP812_13225, DP837_03195, DP859_02865, DP873_09820, DP884_06665, DPA01_04065, DPA17_01430, DPA21_08565, DPA36_04755, DPA81_08695, DPA91_07750, DPB01_05745, DPB58_05530, DPB61_01065, DPB73_06705, DPB80_01010, DPC17_04415, DPC26_12120, DPC30_09225, DPC31_21295, DPC91_07340, DPD28_03410, DPD35_09350, DPD61_05565, DPD71_12990, DPD96_04945, DPE17_03960, DPE32_06850, DPE40_05345, DPE61_01100, DPE66_10470, DPE72_00995, DPE76_10585, DPE91_07335, DPE98_01700, DPF18_01415, DPF55_03280, DPF57_13210, DPF65_05290, DPF69_04225, DPJ06_06690, DPJ07_04770, DPJ18_21730, DPJ21_23060, DPJ65_05985, DPJ69_12010, DPJ81_01030, DPK19_02945, DPK49_18945, DPL03_00940, DPP06_22450, DPP34_02995, DPP49_05140, DPP57_09185, DPP70_04625, DPR96_17095, DPS51_01190, DPS62_02400, DPS71_04035, DPS75_05935, DPS77_06815, DPS96_17080, DPT22_03505, DPT41_07335, DPT74_05620, DPT84_17600, DPT95_03965, DPU11_05080, DPU13_04230, DPY66_15080, DPY81_09375, DPZ06_03150, DPZ23_03295, DPZ65_01920, DPZ67_03455, DPZ88_09965, DPZ95_04280, DQ003_10345, DQ017_21125, DQ037_03770, DQ055_06250, DQ072_13750, DQ076_16795, DQ078_07335, DQ082_06505, DQ790_02530, DQ802_06935, DQ816_09465, DQ836_21070, DQ837_05050, DQ839_09850, DQ845_10875, DQ858_00055, DQ871_15205, DQ880_07325, DQ895_06115, DQ901_09380, DQ919_02340, DQ933_07675, DQ978_14715, DQ985_10135, DQC12_07750, DQC17_05135, DQC26_03870, DQC75_03590, DQC77_06245, DQC81_16925, DQC82_09655, DQC88_01435, DQD09_24240, DQD11_01060, DQD13_03865, DQD28_13095, DQD46_02455, DQD59_03945, DQE08_00395, DQE20_03160, DQE31_05590, DQE33_13015, DQE40_13690, DQE42_08765, DQJ22_01340, DQJ50_08905, DQJ58_03830, DQJ63_05540, DQJ66_02440, DQJ71_08430, DQJ77_01030, DQJ83_05310, DQJ90_08450, DQK19_10845, DQK43_01440, DQK45_13610, DQK53_05425, DQK57_20355, DQK72_09740, DQQ27_15440, DQR39_03355, DQR40_15910, DQR74_07115, DQR85_07280, DQR90_05875, DQS34_02500, DQS55_04230, DQS56_07240, DQS62_04550, DQS71_07420, DQS74_06295, DQS89_04330, DQS98_04035, DQT21_23885, DQT31_01485, DQT40_01975, DQT47_08080, DQT59_08365, DQT67_04950, DQT85_05480, DQY62_10275, DQY86_01365, DQY91_07170, DQZ13_08220, DQZ68_04575, DQZ89_03655, DRA21_00825, DRA23_02570, DRA25_06475, DRA26_15005, DRA30_07960, DRA37_10395, DRD38_06525, DRD40_04685, DRD55_03550, DRD57_02825, DRD79_08175, DRE09_03295, DRE12_04035, DRE16_07695, DRE32_01375, DRE41_16345, DRE46_06240, DRE58_03660, DRE69_05135, DRE85_05775, DRE92_01430, DRF10_01070, DRF26_07295, DRF38_06650, DRK15_08305, DRK36_10935, DRK49_10210, DRK71_14235, DRK91_06225, DRL06_03150, DRL40_04985, DRL53_01550, DRL62_15150, DRL63_08155, DRL65_05050, DRL68_04235, DRL89_01035, DRL96_01185, DRM19_02850, DRM39_10070, DRM41_05515, DRM43_02825, DRM75_09425, DRT10_03415, DRT15_04045, DRT45_11245, DRT49_10675, DRT56_11825, DRT73_05670, DRT76_09345, DRT77_01030, DRU07_04960, DRU14_01025, DRU25_12475, DRU99_06930, DRV08_07615, DRV17_01045, DRV44_09955, DRV53_06735, DRV60_09675, DRV62_04555, DRW94_06120, DRX19_09970, DRX40_11405, DRX43_00440, DRY30_09095, DRY35_08945, DRY53_03870, DRY54_04540, DRY73_06120, DRZ18_11450, DS161_03605, DS168_01430, DS172_06735, DS181_05705, DS188_01805, DS209_03915, DS212_06790, DS228_01025, DS242_04605, DS251_06835, DS266_01340, DS269_03610, DS300_04175, DS306_05655, DS313_01340, DS324_09265, DS336_04600, DS340_09465, DS346_12760, DS368_20110, DS372_11155, DS390_10475, DS438_03885, DS468_03185, DS470_17635, DS485_05030, DS497_03915, DS570_05955, DS572_04860, DS605_10670, DS615_10365, DS651_15910, DS657_02820, DS688_11950, DS689_13215, DS708_08160, DSA82_08035, DSA90_01010, DSA91_03875, DSA99_10695, DSB20_06505, DSB26_11025, DSB29_01055, DSB36_03930, DSB44_04370, DSE94_03270, DSF03_05385, DSF06_15125, DSF13_00100, DSF32_06125, DSF57_04690, DSF62_14785, DSF92_08520, DSF93_06370, DSG01_03865, DSG07_07830, DSG23_03190, DSG69_22660, DSG96_09425, DSH12_03820, DSM73_23085, DSM81_02825, DSM84_01405, DSM85_11930, DSM96_17705, DSN05_00365, DSN18_08025, DSN41_12095, DSQ65_01425, DSQ94_01375, DSR19_03505, DSR78_10785, DSR82_03690, DSR83_08440, DSR88_10410, DSS12_01435, DSS23_05770, DSS37_03960, DSS38_06180, DSS62_22355, DSS75_06400, DSS77_03870, DST00_18010, DST18_17355, DT136_10790, DT138_00540, DT142_21420, DT150_04765, DT159_10485, DT168_04075, DT188_03155, DT193_04870, DT208_00940, DT222_17390, DTE69_04010, DTE86_06360, DTF17_05870, DTF33_02970, DTF39_03290, DTF59_06550, DTF80_05780, DTF85_01005, DTF97_01065, DTG02_02595, DTG11_04415, DTG12_07185, DTG17_13435, DTG24_05555, DTG32_01040, DTG45_10785, DTG47_08540, DTG49_04600, DTG64_04755, DTG68_11905, DTG84_10305, DTH05_03950, DTH08_07170, DTH13_09220, DTT89_02880, DTT93_01025, DTT96_06630, DTT99_02425, DTU24_08720, DTU25_07215, DTU37_09945, DTU48_07545, DTU57_10990, DTU59_09415, DTU60_01035, DTU87_17590, DTV05_07485, DTV16_05625, DTV63_11510, DTV88_17040, DTV89_05155, DTW09_15585, DTW19_02845, DU084_01010, DU092_06545, DU116_00420, DU129_23205, DU131_04030, DU148_01355, DU157_05050, DU159_12015, DU161_00750, DU165_03845, DU168_05890, DU203_03365, DU210_17755, DU215_13830, DU245_04670, DU257_01045, DU748_01890, DU754_02765, DU756_10340, DU758_06015, DU761_12840, DU762_05800, DU777_01410, DU806_14595, DU811_19780, DU834_07665, DU842_16540, DU844_07525, DU852_09995, DU854_02500, DU861_06795, DU873_02415, DU877_10080, DU916_06380, DU946_01030, DU947_01060, DU949_04885, DU954_06100, DUA40_08690, DUA46_06400, DUA52_07680, DUA53_05475, DUA65_00100, DUA91_01005, DUA93_13100, DUB05_08355, DUB53_09680, DUB79_09560, DUB80_04325, DUB82_06390, DUC24_06685, DUC35_07030, DUE02_23055, DUP59_09460, DUP60_01040, DUP67_00775, DUP70_02675, DUP71_10245, DUP74_21535, DUP91_09330, DUQ24_04285, DUQ35_01340, DUQ37_21005, DUQ39_06135, DUQ41_06535, DUQ55_11365, DUQ58_03480, DUQ62_06195, DUQ64_00590, DUQ71_20825, DUQ75_09255, DUQ82_05805, DUQ83_02605, DUQ85_06520, DUR69_18605, DUR85_01530, DUR88_02530, DUR90_07210, DUR96_16820, DUU13_17140, DUU22_03635, DUU35_10475, DUU38_03210, DUU43_07880, DUU44_03135, DUU47_14840, DUU54_17805, DUU71_16085, DUU78_10680, DUU80_06735, DUU92_05345, DUV18_06490, DUV49_17005, DUV51_09990, DUV63_07195, DUV70_07150, DUV72_12440, DUV91_06335, DUW11_05640, DUW53_01400, DV01_10115, DVE93_04900, DVF01_04210, DVF33_03245, DVF55_03535, DVF58_17950, DVG00_04280, DVG02_02975, DVG21_09825, DVG27_01320, DVG41_07205, DVG59_06390, E0U76_06940, E1723_08390, E5N87_02445, EBB74_08700, EBB77_10485, EBB78_09315, EBB82_05950, EBB89_03075, EBC18_06325, EBC34_05750, EBC39_09715, EBC44_20925, EBC68_09380, EBD00_08225, EBD05_16450, EBD20_17095, EBD25_08895, EBD36_08845, EBD43_05210, EBD77_10535, EBJ70_10680, EBJ71_01010, EBJ89_06925, EBJ93_03490, EBJ94_10965, EBK05_09080, EBK23_03750, EBK37_10015, EBK43_02755, EBK60_12435, EBK61_03755, EBO78_16280, EBO89_05830, EBO94_06365, EBP03_08425, EBP04_03395, EBP12_01030, EBP28_05175, EC417_00970, EC420_04945, EC422_04495, EC429_01340, EC434_00995, EC448_08925, EC453_08560, EC471_06365, EC474_02880, EC491_11165, EC492_01205, EC499_09710, ECA60_08605, ECA66_03335, ECA89_08860, ECA94_02770, ECA96_08230, ECB03_04845, ECB08_02380, ECC57_03340, ECC66_14845, ECD11_05680, ECD14_09420, ECD30_08195, ED432_06430, ED436_04010, ED469_03260, ED477_03225, ED486_04135, EDK87_02770, EDL23_06330, EDL36_02800, EDL46_04335, EEA55_06135, EEA59_06700, EEA63_06475, EEA67_03960, EEA91_03575, EEA93_12055, EER52_06640, EER72_05775, EER74_06695, EER75_01000, EER80_15065, EES07_03330, EES08_04495, EES26_06675, EF649_00790, EF653_07695, EF657_16060, EF664_03080, EF665_06445, EF675_05800, EF704_09510, EFQ12_01835, EFQ27_03795, EFQ33_05115, EFQ52_05980, EFQ76_04175, EGL07_15310, EGL44_08725, EGL51_05850, EGL56_03560, EGM22_05950, EGM25_01025, EGM30_19890, EH095_03075, EH102_06310, EH104_01460, EH113_07645, EH130_06335, EHB16_03305, EHB26_04220, EHB38_03060, EHB40_08960, EHB51_06420, EHB53_05245, EHB61_03800, EHB67_09945, EHB73_13145, EHC02_01270, EHC04_15005, EHD17_01205, EHD20_04800, EHD23_03730, EHE53_08440, EHE71_11715, EHE79_04055, EHE81_05630, EHF16_05060, EIE05_03115, EIE15_00620, EIE18_03485, EIE24_02515, EIE42_04075, EIE60_01415, EIK95_05630, EIK98_05950, EIL32_01395, EIW55_09875, EIW58_12505, EIW69_03350, EIW86_10825, EIW93_08525, EK92_17045, ELE06_11115, EUC09_08855, EWC36_09200, EWD86_04885, EXB88_06730, EZX96_06900, F0D09_08975, F0D12_09400, F0D14_05560, F0D29_07175, F0D58_06620, F0H97_08960, F0I33_07995, F0M26_03705, F0M28_05115, F1011_12850, F1J30_00530, F1J33_06075, F1J42_08565, F1J81_05590, F1J82_05610, F1J83_01475, F1J85_04315, F1J88_01420, F1J90_05745, F1K42_02430, F1K59_05050, F2J39_01835, F2J40_00980, F2J48_00940, F2J50_07090, F2J62_03650, F2J66_01280, F2J78_05500, F2K19_06960, F2K22_06105, F2K26_14940, F2V07_07055, F2V23_03890, F2V25_06240, F3550_01355, F3552_00850, F3554_06545, F3591_11485, F3595_10525, F3603_03295, F3626_14980, F3E16_02765, F3E20_04290, F3E26_03740, F3E44_09410, F3E63_09210, F3E73_03605, F3E77_03585, F3E81_09345, F3P41_00915, F3P70_08045, F3P71_01925, F3P74_06490, F3P75_12190, F3P83_04915, F3Q00_03840, F3Q03_05030, F3Q14_02890, F3Q15_02920, F3Q17_04825, F3Q19_00915, F3Q28_06760, F3Q63_04915, F3R19_08235, F3X32_06195, F3X39_18195, F3X53_04150, F3X54_06825, F3X74_09220, F6430_00280, F7247_03195, F7801_03470, F9P04_11415, F9P26_00380, F9Q85_11085, F9W02_05115, F9X76_16290, F9X98_08000, FE718_01360, FE738_01015, FE759_06665, FKN96_12870, FL809_10360, FMV10_12745, FP220_08405, FPF85_03010, FQS21_06835, FQS30_06725, FQS47_00615, FQS59_09325, FR245_13140, FR247_08285, FRM70_05500, FRM79_14805, FRN01_11890, FRN02_05030, FRN09_04175, FRN22_13530, FY061_02260, FZ484_04550, FZ491_03810, FZ502_10420, FZ514_03745, FZ515_13330, FZ516_05975, FZ523_01470, FZ527_01765, FZ528_04325, FZT79_05320, GAW88_10720, GCH48_00965, GCX84_03060, GCY28_22200, GCY29_11985, GDH50_05360, GDH65_00960, GN92_19330, GX90_25025, JF03_19690, JR37_17475, KO25_20305, LB54_08610, LC09_03640, LC85_05730, LH24_08730, LH82_14165, LP13_20040, MB64_17020, NB08_11105, NCTC8272_02769, NU30_22415, PK04_18245, PK95_13970, PV65_06390, QC88_19815, QD42_08685, QD82_16765, QE89_08685, QE93_10380, QF21_24215, R118_18445, R126_08305, R133_19845, RU16_07410, RU46_12145, RY46_16045, RY52_16035, S021_09745, S313_01135, S315_02435, S598_12875, S639_21410, S709_07710, S764_16455, S943_05250, TE27_11430, TL61_17385, VT29_18010, WB97_02550, XJ41_07365, XK54_01525, XK85_16820, XS28_05335, XT48_02390, XZ16_15885, Y847_07225, YA74_10200, YH39_16290, YI72_11450, YJ27_07680, YL04_09955, YL55_19460, YR14_06075, YU10_04620, Z155_07555, Z599_02180, ZA28_00110, ZA51_00590, ZA74_09315, ZD91_01440, ZF07_21535, ZG82_06210, ZO67_01860 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: A0A3U3J7F4, UniProt: Q8ZRA7*PLUS |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 | 名称: AcrB / タイプ: COMPLEX / Entity ID: all / 由来: RECOMBINANT |
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分子量 | 実験値: NO |
由来(天然) | 生物種: Salmonella enterica subsp. enterica serovar Typhi (サルモネラ菌) |
由来(組換発現) | 生物種: Escherichia coli (大腸菌) |
緩衝液 | pH: 7.5 |
試料 | 濃度: 1 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 60 e/Å2 フィルム・検出器のモデル: FEI FALCON III (4k x 4k) |
-解析
ソフトウェア | 名称: PHENIX / バージョン: 1.12_2829: / 分類: 精密化 | ||||||||||||||||||||||||
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
対称性 | 点対称性: C3 (3回回転対称) | ||||||||||||||||||||||||
3次元再構成 | 解像度: 4.6 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 316000 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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